Distribution and diversity of Nosema bombi (Microsporidia: Nosematidae) in the natural populations of bumblebees (Bombus spp.) from West Siberia
- 440 Downloads
Nosema bombi is an obligate intracellular parasite of bumblebees (Hymenoptera, Bombus spp.), which has significant negative effect on individual bumblebees, colony fitness, and development. Recently, several new genetic variants of N. bombi without a defined taxonomic status were identified in natural bumblebee populations from Russia, China, and several European countries, as well as N. ceranae, originally isolated from honey bees, was described in bumblebee species. Thus, it is required to investigate more Nosema variability in bumblebee populations for identifying new genetic Nosema variants. In our study, we used several methods such as total DNA isolation, polymerase chain reaction (PCR) amplification, cloning, sequencing, and comparative and phylogenetic analysis to investigate a prevalence of N. bombi and its diversity in the natural populations of bumblebees across West Siberia. DNA was extracted from intestinal bumblebee tissues. Identification of the parasite was conducted, using PCR with primers specific for the ribosomal RNA gene cluster and methionine aminopeptidase 2 gene of N. bombi followed by sequencing. Seven hundred twenty-seven individual bumblebees belonging to 16 species were tested; 64 specimens revealed presence of the parasite. Prevalence of Nosema bombi infection was different in each region and varied from 4 to 20 %. No infection was found in Bombus agrorum (n = 194) and Bombus equestris (n = 132), both common bumblebees in West Siberia. Three different genetic variants of the same species, N. bombi, were identified. The first variant belonged to N. bombi (AY008373) identified by Fies et al. (J Apicult Res 40:91–96, 2001), second (N. bombi WS2) was identical to the West Siberian variant identified by Szentgyörgyi et al. (Polish Journal of Ecology 59:599–610, 2011), and the last variant, N. bombi WS3, was new. The results led us to suggest that the prevalence of the N. bombi is related to the population structure of bumblebees and distribution of the particular genetic variants of N. bombi.
KeywordsBumblebees Bombus Microsporidia Nosema Ribosomal gene cluster Genetic variant
This study was supported by the Ministry of Education and Science of the Russian Federation (Agreement no. 8124 from 23.07.2012), the 6th EU Framework Programme ALARM GOCE-CT-2003-506675 Integrated Project, the 7th EU Framework Programme STEP—244090, and the Jagiellonian University grant DS/BiNoZ/INoS/761.
- Fantham HB, Porter A (1914) The morphology, biology and economic importance of Nosema bombi, N. sp., parasitic in various humblebees (Bombus spp.). Ann Trop Med Parasit 8:623–638Google Scholar
- Fries I, de Ruijter A, Paxton RJ, da Silva AJ, Slemenda SB, Pieniazek NJ (2001) Molecular characterization of Nosema bombi (Microsporidia: Nosematidae) and a note on its sites of infection in Bombus terrestris (Hymenoptera: Apoidea). J Apicult Res 40:91–96Google Scholar
- Klee J, Besana AM, Genersch E, Gisder S, Nanetti A, Tam DQ, Chinh TX, Puerta F, Ruz JM, Kryger P, Message D, Hatjina F, Korpela S, Fries I, Paxton RJ (2007) Widespread dispersal of the microsporidian Nosema ceranae, an emergent pathogen of the western honey bee, Apis mellifera. J Invertebr Pathol 96:1–10CrossRefPubMedGoogle Scholar
- Li J, Wu J, Cai W, Peng W, An J, Huang J (2008) Comparison of the colony development of two native bumblebee species Bombus ignitus and Bombus lucorum as candidates for commercial pollination in China. J Apicult Res 47:22–26Google Scholar
- Plischuk S, Martin-Hernández R, Prieto L, Lucia M, Botias C, Meana A, Abrahamovich AH, Lange C, Higes M (2009) South American native bumblebees (Hymenoptera: Apidae) infected by Nosema ceranae (Microsporidia), an emerging pathogen of honey bees (Apis mellifera). Env Microbiol Rep 1:131–135CrossRefGoogle Scholar
- Szentgyörgyi H, Blinov A, Eremeeva N, Luzyanin S, Grześ IM, Woyciechowski M (2011) Bumblebees (Bombidae) along pollution gradient—heavy metal accumulation, species diversity, and Nosema bombi infection level. Pol J Ecol 59:599–610Google Scholar