Advertisement

Parasitology Research

, Volume 114, Issue 8, pp 3137–3143 | Cite as

Epidemiological updates and economic losses due to Taenia hydatigena in sheep from Sardinia, Italy

  • A. Scala
  • A. P. Pipia
  • F. Dore
  • G. Sanna
  • C. Tamponi
  • R. Marrosu
  • E. Bandino
  • C. Carmona
  • B. Boufana
  • A. VarcasiaEmail author
Original Paper

Abstract

The aim of this study was to investigate the epidemiology and transmission of Taenia hydatigena in sheep and dogs from Sardinia and the economic estimation of losses due to this metacestodosis in lambs. A total of 7781 Sarda breed lambs were examined at abattoirs for the detection of Cysticercus tenuicollis or necrotic–haemorrhagic tracks of their migration. Morphological and molecular identification of parasites was carried out. Individual faecal samples from 300 dogs were examined for copromicroscopic investigations and coproELISA assay. An overall prevalence of 14.6 % for T. hydatigena cysticercosis was found in the examined lambs. In total, 10,807 parasitary tracks were found, with an abundance of 1.39 and an average intensity of 9.52. The molecular analysis of the isolates showed an overall pairwise nucleotide divergence for the CO1 and ND1 was of 0–3.1 and 0–3.3 %, respectively. Low intra- and interspecific variation was recorded for C. tenuicollis isolates used in this study which suggested the absence of differentiation. Microscopic examination of dog faeces showed a total prevalence of 31.3 % for endoparasites in the examined samples (94/300). Taeniid eggs were found in 8.3 % of the dogs. The results of the monoclonal antibody ATH4 ELISA test showed a prevalence of 11 % (33/300) for T. hydatigena coproantigens. The total economic costs related to cysticercosis amounted to almost € 330,000. The prevalence of C. tenuicollis in 14.6 % of 30–40-day-old lambs highlights the high parasitic pressure by T. hydatigena in the territory of Sardinia, Italy.

Keywords

Taenia hydatigena Cysticercus tenuicollis Cysticercosis Sheep Dogs Sardinia 

References

  1. Abidi S, Nizami W, Khan P, Ahmad M, Irshadullah M (1989) Biochemical characterization of Taenia hydatigena cysticerci from goats and pigs. J Helminthol 63(04):333–337PubMedCrossRefGoogle Scholar
  2. Allan JC, Craig PS, Garcia Noval J, Mencos F, Liu D, Wang Y, Wen H, Zhou P, Stringer R, Rogan M (1992) Coproantigen detection for immunodiagnosis of Echinococcus and taeniasis in dogs and humans. Parasitology 104:347–355PubMedCrossRefGoogle Scholar
  3. Blažek K, Schramlová J, Hulínská D (1985) Pathology of the migration phase of Taenia hydatigena (Pallas, 1766) larvae. Folia Parasitol 32(2):127–137PubMedGoogle Scholar
  4. Bowles J, McManus DP (1993a) NADH dehydrogenase 1 gene sequences compared for species and strains of the genus Echinococcus. Int J Parasitol 23:969–972PubMedCrossRefGoogle Scholar
  5. Bowles J, McManus DP (1993b) Molecular variation in Echinococcus. Acta Trop 53:291–305PubMedCrossRefGoogle Scholar
  6. Bowles J, Blair D, McManus DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 54:165–173PubMedCrossRefGoogle Scholar
  7. Bush AO, Lafferty KD, Lotz JM, Shostak AW (1997) Parasitology meets ecology on its own terms: Margolis et al. revisited. J Parasitol 83(4):575–583PubMedCrossRefGoogle Scholar
  8. Christodoulopoulos G, Theodoropoulos G, Petrakos G (2008) Epidemiological survey of cestode-larva disease in Greek sheep flocks. Vet Parasitol 153:368–373PubMedCrossRefGoogle Scholar
  9. Dew HR (1935) Advances in our knowledge of hydatid disease during the twentieth century. Br Med J 2:620–622PubMedCentralPubMedCrossRefGoogle Scholar
  10. Dore F, Varcasia A, Pipia AP, Sanna G, Pinna Parpaglia ML, Corda A, Romig T, Scala A (2014) Ultrasound as a monitoring tool for cystic echinococcosis in sheep. Vet Parasitol 203(1–2):59–64PubMedCrossRefGoogle Scholar
  11. Edwards GT, Herbert IV (1980) The course of Taenia hydatigena infections in growing pigs and lambs: clinical signs and post-mortem examination. Brit Vet J 136:256–264Google Scholar
  12. Kedra AH, Tkach VV, Swiderski Z, Pawłowski Z (2001) Intraspecific variability among NADH dehydrogenase subunit 1 sequences of Taenia hydatigena. Parasitol Int 50(2):145–148PubMedCrossRefGoogle Scholar
  13. Malgor R, Nonaka N, Basmadjian I, Sakai H, Carambula B, Oku Y, Carmona C, Kamiya M (1997) Coproantigen detection in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay. Int J Parasitol 27:1605–1612PubMedCrossRefGoogle Scholar
  14. Ministero della Salute (2013) Anagrafe Zootecnica istituita presso il CSN dell’Istituto "G. Caporale" di Teramo”Google Scholar
  15. Murrell K, Dorny P, Organization WH (2005) WHO/FAO/OIE guidelines for the surveillance, prevention and control of taeniosis/cysticercosis. OIE, ParisGoogle Scholar
  16. Radfar MH, Zarandi MB, Bamorovat M, Kheirandish R, Sharifi I (2014) Hematological, biochemical and pathological findings in goats naturally infection with Cysticercus tenuicollis. J Parasit Dis 38(1):68–72PubMedCentralPubMedCrossRefGoogle Scholar
  17. Rostami S, Salavati R, Beech RN, Babaei Z, Sharbatkhori M, Baneshi MR, Hajialilo E, Shad H, Harandi MF (2013) Molecular and morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran. J Helminthol 89:150–157PubMedCrossRefGoogle Scholar
  18. Scala A, Marrosu R (1997) Cysticercosis in lambs. Studies on cysticercosis caused by Cysticercus tenuicollis in Sardinia. Obiettivi Doc Vet 18:67–73Google Scholar
  19. Scala A, Mazzette R (2009) Cystic echinococcosis in the sheep: causes of its persistence in Sardinia. Vet Res Commun 33:S41–S45CrossRefGoogle Scholar
  20. Scala A, Garippa G, Varcasia A, Tranquillo VM, Genchi C (2006) Cystic echinococcosis in slaughtered sheep in Sardinia (Italy). Vet Parasitol 135(1):33–38PubMedCrossRefGoogle Scholar
  21. Scala A, Urrai G, Varcasia A, Nicolussi P, Mulas M, Goddi L, Pipia AP, Sanna G, Genchi M, Bandino E (2014) Acute visceral cysticercosis by Taenia hydatigena in lambs and treatment with praziquantel. J Helminthol 14:1–4CrossRefGoogle Scholar
  22. Sweatman GK, Plummer PJG (1957) The biology and pathology of the tapeworm Taenia hydatigena in domestic and wild hosts. Can J Zool 35:93–109CrossRefGoogle Scholar
  23. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729PubMedCentralPubMedCrossRefGoogle Scholar
  24. Thompson RCA, Jenkins DJ (2014) Echinococcus as a model system: biology and epidemiology. Int J Parasitol 44:865–877PubMedCrossRefGoogle Scholar
  25. Trees AJ, Owen RR, Craig PS, Purvis GM (1985) Taenia hydatigena: a cause of persistent liver condemnation in lambs. Vet Rec 11:512–516CrossRefGoogle Scholar
  26. Varcasia A, Garippa G, Scala A (2004) The diagnosis of Echinococcus granulosus in dogs. Parassitologia 46(4):409–412PubMedGoogle Scholar
  27. Varcasia A, Tosciri G, Coccone GN, Pipia AP, Garippa G, Scala A, Damien V, Vural G, Gauci CG, Lightowlers MW (2009) Preliminary field trial of a vaccine against coenurosis caused by Taenia multiceps. Vet Parasitol 162(3–4):285–289PubMedCrossRefGoogle Scholar
  28. Varcasia A, Tanda B, Giobbe M, Solinas C, Pipia AP, Malgor R, Carmona C, Garippa G, Scala A (2011) Cystic echinococcosis in Sardinia: farmers’ knowledge and dog infection in sheep farms. Vet Parasitol 181(2–4):335–340PubMedCrossRefGoogle Scholar
  29. Wang CR, Qiu JH, Zhao JP, Xu LM, Yu WC, Zhu XQ (2006) Prevalence of helminthes in adult dogs in Heilongjiang Province, the People’s Republic of China. Parasitol Res 99:627–630PubMedCrossRefGoogle Scholar
  30. Xhaxhiu D, Kusi I, Rapti D, Kond E, Postoli R, Rinaldi L, Dimitrova ZM, Visser M, Knaus M, Rehbein S (2011) Principal intestinal parasites of dogs in Tirana, Albania. Parasitol Res 108:341–353PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • A. Scala
    • 1
  • A. P. Pipia
    • 1
  • F. Dore
    • 1
  • G. Sanna
    • 1
  • C. Tamponi
    • 1
  • R. Marrosu
    • 2
  • E. Bandino
    • 3
  • C. Carmona
    • 4
  • B. Boufana
    • 5
  • A. Varcasia
    • 1
    Email author
  1. 1.Laboratorio di Parassitologia e Malattie Parassitarie, Ospedale Didattico Veterinario, Dipartimento di Medicina VeterinariaUniversità degli Studi di SassariSassariItaly
  2. 2.ASL N.1SassariItaly
  3. 3.Istituto Zooprofilattico Sperimentale della SardegnaSassariItaly
  4. 4.ParasitologiaUniversidad de la Republica de UruguayMontevideoUruguay
  5. 5.Department of ZoologyUniversity of BenghaziBenghaziLibya

Personalised recommendations