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Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Cestoda, Nippotaeniidea): first data on spermiogenesis and sperm ultrastructure

Abstract

Spermiogenesis and the spermatozoon ultrastructure of the cestode Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Nippotaeniidea), a parasite of Perccottus glenii Dubowski, 1877 (Perciformes: Odontobutidae), have been investigated by means of transmission electron microscopy, cytochemical staining with periodic acid-thiosemicarbazide-silver proteinate (PA-TSC-SP) for glycogen, and electron tomography. The process of spermatozoon formation is characterised by the presence of (1) two centrioles without typical striated rootlets, (2) a single intercentriolar body, (3) a flagellar rotation (free flagellum plus flagellar bud), and (4) a complete proximodistal fusion. The mature spermatozoon of N. mogurndae contains a single helicoidal crested body, one axoneme of the 9 + “1” trepaxonematan structure, parallel cortical microtubules arranged in a ring in the anterior region of the cell, and a spiraled nucleus encircling the axoneme. Intracellular components are situated in a moderately electron-dense cytoplasm, containing glycogen in the principal regions (II, III, IV) of the spermatozoon. Application of electron tomography has revealed a helicoidal nature of the central electron-dense core in the central cylinder of the axoneme in parasitic cestodes for the first time. The patterns of spermiogenesis and spermatozoon ultrastructure resemble most closely those in mesocestoidids and may reflect the relationships between Nippotaeniidea and Mesocestoididae.

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Acknowledgments

We would like to thank Céline Levron (France) and Mikuláš Oros (Slovakia) for invaluable technical help with collecting parasites. We are grateful to the staff of the Laboratory of Electron Microscopy, Institute of Parasitology AS CR, České Budějovice, Czech Republic, for technical assistance. Financial support was provided by the Grant Agency of the Slovak Republic VEGA (project no. 2/0047/11) and the Technology Agency of the Czech Republic (project no. TE01020118 (programme Competence Centre: Electron Microscopy)). The work was realised within a frame of Centre of Excellence for Parasitology (Code ITMS 26220120022) based on the support of the Operational Programme “Research & Development” funded from the European Regional Development Fund (rate 0.1).

Author information

Correspondence to Magdaléna Bruňanská.

Electronic supplementary material

Below is the link to the electronic supplementary material.

The movie shows a 3D colored model and Z walkthrough the axoneme section volume with detail of of the central electrondense core in red colour (correspond to Figures 6 and 7). Single axis tomogram was reconstructed from ∼79 nm thick section and divided to 231 slices and subsequently was applied binning by factor of 2. (MOV 24241 kb)

Movie S1

The movie shows a 3D colored model and Z walkthrough the axoneme section volume with detail of of the central electrondense core in red colour (correspond to Figures 6 and 7). Single axis tomogram was reconstructed from ∼79 nm thick section and divided to 231 slices and subsequently was applied binning by factor of 2. (MOV 24241 kb)

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Bruňanská, M., Bílý, T. & Nebesářová, J. Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Cestoda, Nippotaeniidea): first data on spermiogenesis and sperm ultrastructure. Parasitol Res 114, 1443–1453 (2015) doi:10.1007/s00436-015-4327-0

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Keywords

  • Nippotaeniidea
  • Nippotaenia mogurndae
  • Spermiogenesis
  • Spermatozoon
  • Ultrastructure