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Parasitology Research

, Volume 114, Issue 3, pp 861–872 | Cite as

Histopathological and ultrastructural studies of a Henneguya species (Myxozoa: Myxosporea) infesting the intestine of Clarias gariepinus from Benin (West Africa)

  • Nounagnon Darius TossaviEmail author
  • Adam Gbankoto
  • Akadiri Yessoufou
  • Marie-Line Escande
  • Gorand Dimitri
  • Anne-Cécile Ribou
  • Kabirou Moutaïrou
  • Gilbert Nestor Sakiti
Original Paper

Abstract

Fish culture is the best alternative to fill the gap between natural fish catches and estimated needs of populations in animal protein consumption. In West Africa, this goal required to have suitable fishes for aquaculture which are Clariidae and Tilapia. Clarias gariepinus (Clariidae) fetches a higher price than tilapias as it can be sold alive at the market but a high infestation by Henneguya leads to decrease this commercial value. Those reasons lead us to perform studies on seasonal variations, histopathological aspects and life cycle of Henneguya sp. infecting the intestine of C. gariepinus using light and electron microscope. From November 2011 to December 2012, 339 specimens were collected from Ouémé River (Benin) and examined. An overall prevalence of 7.37 % was recorded for plasmodia of Henneguya sp. Parasite occurrence did not vary significantly between seasons (χ2 = 12.235; df = 3; p > 0.05), nor sexes (χ2 = 2.992; df = 7; p > 0.05) while differences were significant between classes of weight (χ2 = 39.929; df = 5; p < 0.05). The highest prevalence was recorded in host ranging from 300 to 374 g. Histopathological analysis showed that the mass continuous development of the plasmodium produced thickening of the intestine wall and compressed neighboring tissues and destroyed villi and smooth muscle layers. The stages of the parasite development including sporogenesis, capsulogenesis, and valvogenesis were asynchronous. Investigations are still running by molecular approaches in order to identify accurately this species.

Keywords

Clarias gariepinus Henneguya sp Prevalence Histopathology Intestine Electron microscope 

Notes

Acknowledgments

NDT is thankful to Prof Thierry NOGUER, headmaster of the Institut de Modélisation et d’Analyse en Géo-Environnement et Santé (IMAGES) of UPVD, for taking a great interest in his work and for providing laboratory facilities during his stay at Perpignan.

References

  1. Abdel-Ghaffar F, Abdel-Aziz A, Ezz El-Din N, Naas S (1995) Light and electron microscopic studies on Henneguya branchialis (Ashmawy et al. 1989) (Myxosporea: Myxozoa) infecting the catfish Clarias gariepinus in the River Nile Egypt. J Union Arab Biol 3(A):113–133Google Scholar
  2. Abdel-Ghaffar F, Ibrahim EA, Bashtar A, Ali MA (1998) Myxosporidia infecting saline and freshwater fishes of Qarun and Wadi El-Raiyan lakes, Egypt. J Egypt Ger Soc Zool (D) 26:209–229Google Scholar
  3. Abdel-Ghaffar F, Abdel-Baki AS, Bayoumy E, Bashtar AR, Al-Quraishy S, Morsy KS, Alghamdy A, Mehlhorn H (2008) Light and electron microscopic study on Henneguya suprabranchiae Landsberg, 1987 (Myxozoa: Myxosporea) infecting Oreochromis niloticus, a new host record. Parasitol Res 103:609–617CrossRefPubMedGoogle Scholar
  4. Adriano EA, Arana S, Cordeiro NS (2005) Histology, ultrastructure and prevalence of Henneguya plaractus (Myxosporea) infecting the gills of Plaractus mesopotamicus (Characidae) cultivated in Brazil. Dis Aquat Org 64:229–235CrossRefPubMedGoogle Scholar
  5. Adriano EA, Arana S, Alves AL, Silva MRM, Ceccarelli PS, Henrique-Silva F, Maia AAM (2009) Myxobolus cordeiroi n. sp., a parasite of Zungaro jahu (Siluriformes: Pimelodiade) from Brazilian Pantanal: morphology, phylogeny and histopathology. Vet Parasitol 162:221–229CrossRefPubMedGoogle Scholar
  6. Ali MA (1999) Henneguya ghaffari sp. n. (Myxozoa: Myxosporea) infecting the Nile perch Lates niloticus (Teleostei: Centropomidae). Dis Aquat Org 38:225–230CrossRefPubMedGoogle Scholar
  7. Ali MA, Al-Rasheid AS, Sakran T, Abdel-baki AS, Abdel-Ghaffar FA (2002) Some species of the genus Myxobolus (Myxozoa: Myxosporea) infecting freshwater fishes of the River Nile, Egypt. Parasitol Res 88:9–15PubMedGoogle Scholar
  8. Ali MA, Abdel-Baki AS, Sakran T, Entzeroth R, Abdel-Ghaffar F (2007) Myxobolus lubati n. sp. (Myxosporea: Myxobolidae), a new parasite of haffara seabream Rhabdosargus haffara (Forsskal, 1775), Red Sea, Egypt: a light and transmission electron microscopy. Parasitol Res 100:819–827CrossRefPubMedGoogle Scholar
  9. Alvarez-Pellitero P, Palenzuela O, Sitja-Bobadilla A (2008) Histopathology and cellular response in Enteromyxum leei (Myxozoa) infections of Diplodus puntazzo (Teleostei). Parasitol Int 57:110–120CrossRefPubMedGoogle Scholar
  10. Anon (Anonymous): Direction des pêches (2011) Rapport Annuel d’Activité. Ministère de l’Agriculture, de l’Elevage et de la Pêche, République du Bénin. 72pGoogle Scholar
  11. Azevedo C, Matos E (1989) Some ultrastructural data on the spore development in a Henneguya sp. parasite of the gill of a Brazilian fish. Parasitol Res 76:131–134CrossRefPubMedGoogle Scholar
  12. Azevedo C, Lom J, Corral L (1989) Ultrastructural aspects of Myxidium giardi (Myxozoa: Myxosporea) parasite of the European eel Anguilla anguilla. Dis Aquat Org 6:55–61Google Scholar
  13. Bahri S (2008) Abnormal forms of Myxobolus bizerti and Myxobolus mülleri (Myxosporea: Bivalvulida) spores with caudal appendages. Bull Eur Assoc Fish Pathol 20:252–255Google Scholar
  14. Barassa B, Adriano EA, Cordeiro NS, Arana S, Ceccarelli PS (2012) Morphology and host–parasite interaction of Henneguya azevedoi n. sp., parasite of gills of Leporinus obtusidens from Mogi-Guaçu River, Brazil. Parasitol Res 110:887–894CrossRefPubMedGoogle Scholar
  15. Carriero MM, Adriano EA, Silva MRM, Ceccarelli PS, Maia AM (2013) Molecular phylogeny of Myxobolus and Henneguya genera with several New South America species. Plos ONE 8:e73713CrossRefPubMedCentralPubMedGoogle Scholar
  16. Current WL, Janovy J Jr (1976) Ultrastructure of interlamellar Henneguya exilis in the channel catfish. J Parasitol 62:975–998CrossRefPubMedGoogle Scholar
  17. Current WL, Janovy J Jr (1977) Sporogenesis in Henneguya exilis infecting the Channel catfish: an ultrastructural study. Protistologica 13:157–167Google Scholar
  18. Current WL, Janovy J Jr (1978) Comparative study of ultrastructure of interlamellar and intralamellar types of Henneguya exilis Kudo from channel catfish. J Protozool 25:56–65CrossRefPubMedGoogle Scholar
  19. Current WL, Janovy J Jr, Knight SA (1979) Myxosoma funduli Kudo (Myxosporida) in Fundulus kansae : ultrastructure of the plasmodium wall and sporogenesis. J Protozool 26:574–583CrossRefGoogle Scholar
  20. Desser SS, Paterson WB (1978) Ultrastructural and cytochemical observation on sporogenesis of Myxobolus sp. (Myxosporidia) from common shiner Natropis coronutus. J Protozool 25:314–326CrossRefPubMedGoogle Scholar
  21. Desser SS, Molnar K, Weiller I (1983) Ultrastructure of sporogenesis of Thelohanellus nikolskii Akkhmerov, 1955 (Myxozoa: Myxosporea) from the common carp Cyprinus carpio. J Parasitol 69:504–518CrossRefGoogle Scholar
  22. Eiras JC (2002) Synopsis of the species of the genus Henneguya Thélohan, 1892 (Myxozoa: Myxosporea: Myxobolidae). Syst Parasitol 52:43–54CrossRefPubMedGoogle Scholar
  23. Eiras JC, Adriano EA (2012) A checklist of new species of Henneguya Thélohan, 1892 (Myxozoa: Myxosporea: Myxobolidae) described between 2002 and 2012. Syst Parasitol 83:95–104CrossRefPubMedGoogle Scholar
  24. El-Mansy A, Bashtar AR (2002) Histopathological and ultrastructural studies of Henneguya suprabranchiae Landsberg, 1987 (Myxosporea: Myxobolidae) parasitizing the suprabranchial organ. Parasitol Res 88:617–626CrossRefPubMedGoogle Scholar
  25. El-Matbouli M, Hoffmann RW (1989) Experimental transmission of two Myxobolus spp developing bisporogony via tubificid worms. Parasitol Res 75:461–464CrossRefPubMedGoogle Scholar
  26. El-Matbouli M, Fischer-Scherl T, Hoffmann RW (1990) Light and electron microscopic studies on Myxobolus cotti El-Matbouli and Hoffmann, 1987 infecting the central nervous system of the bullhead (Cottus gobio). Parasitol Res 76:219–227CrossRefPubMedGoogle Scholar
  27. Ernst II, Whttingtton D, Corneillte S, Talbot C (2005) Effects of temperature, salinity, desiccation and chemical treatments on egg embryonation and hatching success of Benedenia seriolae (Monogenea: Capsalidae), a parasite of farmed Seriola spp. J Fish Dis 28:157–164Google Scholar
  28. Estensoro I, Redondo JM, Alvarez-Pellitero P, Sitja-Bobadilla A (2010) Novel horizontal transmission route for Enteromyxum leei (Myxozoa) by anal intubation of gilthead sea bream Sparus aurata. Dis Aquat Org 92:51–58CrossRefPubMedGoogle Scholar
  29. Evans DH, Piermarini PM, Choe KP (2005) The multifunctional fish gill: dominant site of gas exhange, osmoregulation, acid–base regulation and excretion of nitrogenous waste. Physiol Revs 85:97–177CrossRefGoogle Scholar
  30. FAO (2012) La situation mondiale des pêches et de l’aquaculture 2012, Rome, 241pGoogle Scholar
  31. Feist SW, Longshaw M (2005) Myxozoan diseases of fish and effects on host populations. Acta Zool 51:758–760Google Scholar
  32. Feist SW, Longshaw M (2006) Phylum myxozoa. In Woo PTK editor. Fish diseases and disorders. Protozoan and metazoan infections. Vol. 1, 2nd ed. CAB International, Oxfordshire, pp 230–296CrossRefGoogle Scholar
  33. Fiala I (2006) The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 36:1521–1534CrossRefPubMedGoogle Scholar
  34. Gbankoto A, Pampoulie C, Marques A, Sakiti GN (2001a) Occurrence of myxosporean parasites in the gills of two tilapia species from Lake Nokoué (Benin, West Africa): effect of host size and sex, and seasonal patterns of infection. Dis Aquat Org 44:217–222CrossRefPubMedGoogle Scholar
  35. Gbankoto A, Pampoulie C, Marques A, Sakiti GN (2001b) Myxobolus dahomeyensis infection in ovaries of tilapia species from Bénin (West Africa). J Fish Biol 58:883–886CrossRefGoogle Scholar
  36. Gbankoto A, Pampoulie C, Marques A, Sakiti GN, Dramane KL (2003) Infection patterns of Myxobolus heterospora in two tilapia species (Teleostei: Cichlidae) and its potential effects. Dis Aquat Org 55:125–131CrossRefPubMedGoogle Scholar
  37. Ghaffar FA, El-Shahawi G, Naas S (1995) Myxosporidia infecting some Nile fishes in Egypt. Parasitol Res 81:163–166CrossRefPubMedGoogle Scholar
  38. Haaparanta A, Valtonen ET, Hoffman RW (1994) Pathogenicity and seasonal occurrence of Hennuguya creplini (Protozoa, Myxosporea) on the gills of perch Perca fluviatilis in central Finland. Dis Aquat Org 20:15–22CrossRefGoogle Scholar
  39. Hwang PP, Lee TH (2007) News insights into fish ion regulation and mitochondrion-rich cells. Comp Biochem Physiol A 148:479–497CrossRefGoogle Scholar
  40. Iwanowicz DD, Iwanowicz LR, Howerth EW, Schill WB, Blazer VS, Johnson RL (2013) Characterization of a new myxozoan species (Myxozoa: Myxobolidae: Myxosporea) in large scale stonerollers (Campostoma oligolepis) from the mobile river basin (Alabama). J Parasitol 99:102–111CrossRefPubMedGoogle Scholar
  41. Karasov WH, Hume ID (1997) Vertebrate gastrointestinal system. In: Dantzler WH (ed) In the handbook of physiology: comparative physiology, vol. 1. Oxford University Press, Oxford, pp 409–480Google Scholar
  42. Kent ML, Andree KB, Bartholomew JL, El-Matbouli M, Desser SS, Devlin RH, Feist SW, Hedrick RP, Hoffman RW, Khattra J, Halllett SL, Lester RJG, Longshaw M, Palenzeula O, Siddall ME, Xiao C (2001) Recent advances in our knowledge of the Myxozoa. J Eukaryot Microbiol 48(4):395–413CrossRefPubMedGoogle Scholar
  43. Khidr AA, Said AE, Samak OAA, Sheref SEA (2012) The impacts of ecological factors on prevalence, mean intensity and seasonal changes of the monogenean gill parasite, Microcotyloides sp., infesting the Terapon puta fish inhabiting coastal region of Mediterranean Sea at Damietta region. J Basic & Appl Zool 65:109–115Google Scholar
  44. Landsberg JH (1985) Myxosporean infections in cultured tilapias in Israel. J Protozool 32:194–201CrossRefPubMedGoogle Scholar
  45. Lazard J, Legendre M (1994) La pisciculture africaine: enjeux et problèmes de recherche. Cah Agri 3:83–92Google Scholar
  46. Li Y, Sato H, Kamata Y, Ohnishi T, Sugita-Konishi Y (2012) Three novel myxobolid species of genera Henneguya and Myxobolus (Myxosporea: Bivalvuvulida) from marine fish in Japan. Parasitol Res 111:819–826CrossRefPubMedGoogle Scholar
  47. Liu Y, Gu ZM, Luo LY (2010) Some additional data to the occurrence, morphology and validity of Myxobolus turpisrotundus Zhang, 2009 (Myxozoa: Myxosporea). Parasitol Res 107:67–73CrossRefPubMedGoogle Scholar
  48. Lodish H, Berk A, Matsudaira P, Kaiser CA, Krieger M, Scott MP, Zipursky L, Darnell J (2005) Biologie Moléculaire de la Cellule, Ed. de boeck, Espagne. 973pGoogle Scholar
  49. Lom J, Arthur JR (1989) A guideline for the preparation of species description in Myxosporea. J Fish Dis 12:151–156CrossRefGoogle Scholar
  50. Lom J, de Puytorac P (1965) Studies on the myxosporidian ultrastructure and polar capsule development. Protistol 1:53–65Google Scholar
  51. Lom J, Dykova I (1992a) Protozoan parasites of fishes. Developments in Aquaculture and Fisheries Science Vol.26, Elsevier, 315pGoogle Scholar
  52. Lom J, Dykova I (1992b) Fine-structure of triactinomyxon early stages and sporogony myxosporean and actinosporean features compared. J Protozool 39:16–27CrossRefGoogle Scholar
  53. Lom J, Dykova I (1994) Studies on protozoan parasites of Australian fishes III. Species of genus Myxobolus Bütschli, 1882. Eur J Protistol 30:431–439CrossRefGoogle Scholar
  54. Lom J, Dykovà I (2006) Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 53:1–36CrossRefPubMedGoogle Scholar
  55. Lom J, Noble ER (1984) Revised classification of the class Myxosporea Butschli, 1881. Folia Parasitol (Praha) 31:193–205Google Scholar
  56. Lom J, Feist SW, Dykova I, Kepr T (1989) Brain myxoboliasis of bullhead, Cottus gobio L., due to Myxobolus jiroveci sp. n.: light and electron microscope observations. J Fish Dis 12:15–27CrossRefGoogle Scholar
  57. Lom J, Rohde K, Dykova I (1992) Studies on protozoan parasites of Australian fishes I. New species of the genera Coccomyxa Léger et Hess, 1907, Ortholinea Shulman, 1962 and Kudoa Meglitsch, 1947 (Myxozoa, Myxosporea). Folia Parasitol 39:289–306Google Scholar
  58. Matos E, Tajdari J, Azevedo C (2005) Ultrastructural studies of Henneguya rhamdia n. sp. (Myxozoa) a parasite from the Amazon teleost fish, Rhamdia quelen (Pimelodidae). J Eukaryot Microbiol 52(6):532–537CrossRefPubMedGoogle Scholar
  59. McCraren JP, Landolt ML, Hoffman GL, Meyer FP (1975) Variation in response of channel catfish to Henneguya sp infections (Protozoa: Myxosporidia). J Wildl Dis 11:2–7CrossRefGoogle Scholar
  60. Milanin T, Eiras JC, Arana S, Maia AAM, Alves AL, Silva MRM, Carriero MM, Ceccarelli PS, Adriano EA (2010) Phylogeny, ultrastructure, histopathology and prevalence of Myxobolus oliveirai sp. nov., a parasite of Brycon hilarii (Characidae) in the Pantanal wetland, Brazil. Mem Inst Oswaldo Cruz 105:762–769CrossRefPubMedGoogle Scholar
  61. Molnár K (1979) Myxobolus pavlovskii (Achmerov, 1954) (Myxosporidia) infection in the silver carp and bighead. Acta Vet Hung 3:207–2l6Google Scholar
  62. Molnár K, Eszterbauer E, Székely C, Dán A, Harrach B (2002) Morphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish. J Fish Dis 25:643–652CrossRefGoogle Scholar
  63. Morsy K, Abdel-Ghaffar F, Bashtar A-R, Mehlhorn H, Al Quraishy S, Abdel-Gaber R (2012) Morphology and small subunit ribosomal DNA sequence of Henneguya suprabranchiae (Myxozoa), a parasite of the catfish Clarias gariepinus (Clariidae) from the River Nile, Egypt. Parasitol Res 111:1423–1435CrossRefPubMedGoogle Scholar
  64. Munoz P, Sitja-Bobadilla A, Alvarez-Pellitero P (2000) Antigenic characterization of Sphaerospora dicentrarchi (Myxospora: Bivalvulida), a parasite from European seabass Dicentrarchus labrax (Teleostei: Serranidae). Dis Aquat Org 40:117–124CrossRefPubMedGoogle Scholar
  65. Muzzall PM (1995) Distribution of Myxobolus scleroperca (Myxobolidae: Myxosporea) in yellow perch (Perca flavescens) in the Great Lakes. J Parasitol 81:498–499CrossRefPubMedGoogle Scholar
  66. Obiekezie AI, Enyenihi MK (1988) Henneguya chrysichthyi sp, nov. (Protozoa: Myxozoa) from the gills of estuarine catfish Chrysichthys nigrodigitatus (Lacepède) (Pisces: Bagridae) in Nigeria. J. Afr Zool 102:33–42Google Scholar
  67. Okaeme AN, Obiekeziz AI, Lehman J, Antai EE, Madu CT (1988) Parasites and diseases of cultured fish of Lake Kainji area Nigeria. J Fish Biol 32:479–481CrossRefGoogle Scholar
  68. Palenzuela O (2006) Myxozoan infections in Mediterranean mariculture. Parasitologia 48:27–29Google Scholar
  69. Pulsford A, Matthews RA (1982) An ultrastructure study of Myxobolus exiguus Thelohan, 1895 (Myxosporea) from gray mullet, Crenimugil labrosus (Risso). J Fish Dis 5:509–526CrossRefGoogle Scholar
  70. Rigos G, Katharios P (2010) Pathological obstacles of newly-introduced fish species in Mediterranean mariculture: a review. Rev Fish Biol Fish 20:47–70CrossRefGoogle Scholar
  71. Sakiti GN, Blanc E, Marques A, Bouix G (1991) Myxosporidies (Myxozoa : Myxosporea) du genre Myxobolus Bütschli, 1882, parasites de poissons Cichlidae du Lac Nokoué au Bénin (Afrique de l’Ouest). J Afr Zool 105:173–186Google Scholar
  72. Sakiti GN, Tarer V, Jacquemin D, Marques A (1996) Présence en Méditerranée occidentale d’une Myxosporidie histozoïque pathogène dans les élevages de daurade, Sparus aurata L. Ann Sci Nat B 17:123–127Google Scholar
  73. Sakiti N, Kabré G, Marques A, Dossou C (1999) Gill diseases caused by Myxozoa in fishes of Inland waters of the south of Benin. J Eukaryot Microbiol 46:15AGoogle Scholar
  74. Schmahl G, Mehlhorn H, Taraschewski H (1989) Treatment of fish parasites. 5. The effects of sym. Triazinone (Toltrazuril) on skin parasitic Ciliophora (Ichthyophthirius multifilis Fouquet, 1876, Glossatella amoebae Grenfell, 1884, and Trichodina sp. Ehrenberg, 1831). Eur J Protistol 24:152–161CrossRefPubMedGoogle Scholar
  75. Stehr C (1986) Sporogenesis of the myxosporean Kudoa paniformis Kabata & Whitaker, 1981 and Kudoa thyrsites (Gilchrist, 1924) in the muscle of Pacific whiting, Merluccius productus (Ayres): an ultrastructural study. J Fish Dis 9:505–517CrossRefGoogle Scholar
  76. Swanson C (1998) Interactive effects of metabolic rate, activity, growth and osmoregulation in the euryhaline milkfish (Chanos chanos). J Exp Biol 201:3355–3366PubMedGoogle Scholar
  77. Tossavi ND, Gbankoto A, Adite A, Ibikounle M, Grunau C, Sakiti GN (2014) Metazoan parasite communities of Catfishes (Teleostei: Siluridae) in Benin: West Africa. Parasitol Res 113:3973–3983CrossRefPubMedGoogle Scholar
  78. Tseng YC, Hwang PP (2008) Some insights into metabolism for osmoregulation in fish. Comp Biochem Physiol C 148:419–429Google Scholar
  79. Viozzi GP, Flores VR (2003) Myxidium biliare sp. n. (Myxozoa) from gall bladder of Galaxias maculates (Osmeriformes: Galaxiidae) in Patagonia (Argentina). Folia Parasitol 50:190–194CrossRefPubMedGoogle Scholar
  80. Von Heijne G (1999) Recent advances in the understanding of membrane protein assembly and structure. Q Rev Biophys 32:285–307CrossRefGoogle Scholar
  81. Wieser W, Medgyesy N (1991) Metabolic rate and cost of growth in juvenile pike (Esox lucius L.) and fresh perch (Perca fluviatilis L.): the use of energy budgets as indicators of environmental change. Ecologica 87:500–505Google Scholar
  82. Zhang JY, Al-Quraishy S, Abdel-Baki AS (2014) The morphological and molecular characterization of Myxobolus khaliji n. sp. (Myxozoa: Myxosporea) from the double bar sea bream Acanthopagrus bifasciatus (Forsskål, 1775) in the Arabian Gulf, Saudi Arabia. Parasitol Res 113:2177–2183CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Nounagnon Darius Tossavi
    • 1
    Email author
  • Adam Gbankoto
    • 2
    • 4
  • Akadiri Yessoufou
    • 3
  • Marie-Line Escande
    • 5
  • Gorand Dimitri
    • 6
  • Anne-Cécile Ribou
    • 4
  • Kabirou Moutaïrou
    • 3
  • Gilbert Nestor Sakiti
    • 1
  1. 1.Département de Zoologie, Faculté des Sciences et TechniquesUniversité d’Abomey-CalaviCotonouBénin
  2. 2.Département de Physiologie Animale, Faculté des Sciences et TechniquesUniversité d’Abomey-CalaviCotonouBénin
  3. 3.Département de Biochimie et Biologie Cellulaire, Faculté des Sciences et TechniquesUniversité d’Abomey-CalaviCotonouBénin
  4. 4.Institut de Modélisation et d’Analyse en Géo-Environnement et Santé, EA 4218Université de Perpignan Via DomitiaPerpignanFrance
  5. 5.Observatoire Océanologique de BanyulsUPMC Université Paris 06, UMR 7232Banyuls-sur-MerFrance
  6. 6.Centre de caractérisation de la matière (PROMES), TecnosudUniversité de Perpignan I.M.P./CNRSPerpignanFrance

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