Parasitology Research

, Volume 114, Issue 3, pp 861–872 | Cite as

Histopathological and ultrastructural studies of a Henneguya species (Myxozoa: Myxosporea) infesting the intestine of Clarias gariepinus from Benin (West Africa)

  • Nounagnon Darius TossaviEmail author
  • Adam Gbankoto
  • Akadiri Yessoufou
  • Marie-Line Escande
  • Gorand Dimitri
  • Anne-Cécile Ribou
  • Kabirou Moutaïrou
  • Gilbert Nestor Sakiti
Original Paper


Fish culture is the best alternative to fill the gap between natural fish catches and estimated needs of populations in animal protein consumption. In West Africa, this goal required to have suitable fishes for aquaculture which are Clariidae and Tilapia. Clarias gariepinus (Clariidae) fetches a higher price than tilapias as it can be sold alive at the market but a high infestation by Henneguya leads to decrease this commercial value. Those reasons lead us to perform studies on seasonal variations, histopathological aspects and life cycle of Henneguya sp. infecting the intestine of C. gariepinus using light and electron microscope. From November 2011 to December 2012, 339 specimens were collected from Ouémé River (Benin) and examined. An overall prevalence of 7.37 % was recorded for plasmodia of Henneguya sp. Parasite occurrence did not vary significantly between seasons (χ2 = 12.235; df = 3; p > 0.05), nor sexes (χ2 = 2.992; df = 7; p > 0.05) while differences were significant between classes of weight (χ2 = 39.929; df = 5; p < 0.05). The highest prevalence was recorded in host ranging from 300 to 374 g. Histopathological analysis showed that the mass continuous development of the plasmodium produced thickening of the intestine wall and compressed neighboring tissues and destroyed villi and smooth muscle layers. The stages of the parasite development including sporogenesis, capsulogenesis, and valvogenesis were asynchronous. Investigations are still running by molecular approaches in order to identify accurately this species.


Clarias gariepinus Henneguya sp Prevalence Histopathology Intestine Electron microscope 



NDT is thankful to Prof Thierry NOGUER, headmaster of the Institut de Modélisation et d’Analyse en Géo-Environnement et Santé (IMAGES) of UPVD, for taking a great interest in his work and for providing laboratory facilities during his stay at Perpignan.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Nounagnon Darius Tossavi
    • 1
    Email author
  • Adam Gbankoto
    • 2
    • 4
  • Akadiri Yessoufou
    • 3
  • Marie-Line Escande
    • 5
  • Gorand Dimitri
    • 6
  • Anne-Cécile Ribou
    • 4
  • Kabirou Moutaïrou
    • 3
  • Gilbert Nestor Sakiti
    • 1
  1. 1.Département de Zoologie, Faculté des Sciences et TechniquesUniversité d’Abomey-CalaviCotonouBénin
  2. 2.Département de Physiologie Animale, Faculté des Sciences et TechniquesUniversité d’Abomey-CalaviCotonouBénin
  3. 3.Département de Biochimie et Biologie Cellulaire, Faculté des Sciences et TechniquesUniversité d’Abomey-CalaviCotonouBénin
  4. 4.Institut de Modélisation et d’Analyse en Géo-Environnement et Santé, EA 4218Université de Perpignan Via DomitiaPerpignanFrance
  5. 5.Observatoire Océanologique de BanyulsUPMC Université Paris 06, UMR 7232Banyuls-sur-MerFrance
  6. 6.Centre de caractérisation de la matière (PROMES), TecnosudUniversité de Perpignan I.M.P./CNRSPerpignanFrance

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