Selection, resistance risk assessment, and reversion toward susceptibility of pyriproxyfen in Musca domestica L.
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Pyriproxyfen, a juvenile hormone mimic, is an effective larvicide against many pests of veterinary and public health importance. Pyriproxyfen is a biorational insecticide having many environmentally friendly attributes that make it compatible with integrated pest management programs. This experiment was performed for the assessment of resistance evolution and reversion toward susceptibility of Musca domestica to pyriproxyfen. Repeated selection at successive generations resulted in 5.09- and 130-fold increase in lethal concentration 50 (LC50) compared to field and susceptible strain, respectively. A significant decline after 22 generations without selection suggesting resistance to pyriproxyfen was unstable in M. domestica. Realized heritability (h 2) of resistance to pyriproxyfen was 0.035 in pyriproxyfen-selected strain of M. domestica. The projected rate of resistance development indicated that, if slope = 1.28 and h 2 = 0.035, then 46–21 generations are required for 10-fold increase in LC50 at 50–90 % selection intensity. These findings suggest that a risk for resistance development to pyriproxyfen occurred in M. domestica under continuous selection pressure. Pyriproxyfen susceptibility reversed when its application is ceased for a specified duration.
KeywordsHouse fly Pyriproxyfen Resistance Heritability Stability
The authors are highly thankful to Rachel Lehrer, strategist at Continuum Innovation, 1220 Washington St. West Newton, MA 02465, for critical review of manuscript to improve English language.
- Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics, 4th edition. Longman, New YorkGoogle Scholar
- Finney D (1971) A statistical treatment of the sigmoid response curve. Probit analysis, 3rd edn. Cambridge University Press, London, p 333Google Scholar
- Invest J, Lucas J (2008) Pyriproxyfen as a mosquito larvicide. Sixth International Conference on Urban Pests, VeszprémGoogle Scholar
- Mojaver M, Bandani A (2010) Effects of the insect growth regulator pyriproxyfen on immature stages of sunn pest, Eurygaster integriceps Puton (Heteroptera: Scutelleridae). Munis Entomol Zool 5:187–197Google Scholar
- Roush RT, Croft B (1986) Experimental population genetics and ecological studies of pesticide resistance in insects and mites. In: Pesticide resistance: strategies and tactics for management. Nat. Acad. Press, Washington, pp 257–270Google Scholar
- Slansky F, Scriber J (1985) Food consumption and utilization. Compr Insect Physiol, Biochem Pharmacol 4:87–163Google Scholar
- Tabashnik BE (1992) Resistance risk assessment: realized heritability of resistance to Bacillus thuringiensis in diamondback moth (Lepidoptera: Plutellidae), tobacco budworm (Lepidoptera: Noctuidae), and Colorado potato beetle (Coleoptera: Chrysomelidae). J Econ Entomol 85:1551–1559CrossRefGoogle Scholar