Parasitology Research

, Volume 113, Issue 11, pp 4315–4319 | Cite as

Variation in adult longevity of Culex pipiens f. pipiens, vector of the West Nile Virus

  • S. S. AndreadisEmail author
  • O. C. Dimotsiou
  • M. Savopoulou-Soultani
Short Communication


The common house mosquito, Culex pipiens (Diptera: Culicidae), which is considered the primary bridge vector of West Nile Virus (WNV) to humans, is a wide spread insect pest with medical importance and consists of two distinct bioforms, Cx. pipiens f. pipiens and Cx. pipiens f. molestus. Here, we consider the adult lifespan of male and female Cx. pipiens f. pipiens under controlled conditions at five constant temperature regimes (15, 20, 25, 27.5, and 30 °C). Our results show that adult longevity was affected by temperature, as it significantly decreased with increase in temperature. At the highest tested temperature, mean adult longevity did not exceed 12 days for both sexes and thus makes impossible the risk of WNV transmission. On the other hand at the lowest temperature, longevity was extremely high with some individuals surviving up to 129 and 132 days, males and females, respectively, and thus enable them to function as potential vectors of WNV for a prolonged period of time. As far as sex is concerned, adult females displayed a 1.2–1.4-fold longer longevity compared to the male ones. However, this difference was significant only at the lowest and highest tested temperature regime. This information is useful in determining the critical temperatures which may affect the distribution of Cx. pipiens and consequently the risk of WNV transmission. Moreover, the effect of environmental temperature should be considered when evaluating the abundance of these species.


Temperature Sex Adult longevity Culex pipiens complex West Nile virus 



The authors thank Charoula Christopoulou for laboratory assistance. This work was partially funded by the Aristotle University of Thessaloniki Research Committee.


  1. (HCDCP) Hellenic Centre for Disease Control and Prevention (2013) Weekly Epidemiological Report West Nile Virus Infection, Greece (Report 06/11/2013). Accessed 30 April 2014
  2. Anderson JF, Main AJ (2006) Importance of vertical and horizontal transmission of West Nile Virus by Culex pipiens in the Northeastern United States. J Infect Dis 194:1577–1579PubMedCrossRefGoogle Scholar
  3. Andreadis SS, Dimotsiou OC, Christopoulou CC, Soultani CV, Savopoulou-Soultani M (2007) Mosquito species recorded in the prefecture of Chalkidiki in 2007. In: Kapaxidi, E, Vasiliou L, Michalaki M (eds) Proceedings of 12th Panhellenic Congress of Entomology, Larnaca, Cyprus, pp 263–266 (English summary)Google Scholar
  4. Andreadis TG (2012) The contribution of Culex pipiens complex mosquitoes to transmission and persistence of West Nile virus in North America. J Am Mosq Control Assoc 28:137–151PubMedCrossRefGoogle Scholar
  5. Antoniadis A, Alexiou-Daniel S, Malissiovas N, Doutsos J, Polyzoni T, LeDuc JW, Peters CJ, Saviolakis G (1990) Seroepidemiological survey for antibodies to arboviruses in Greece. Arch Virol Suppl 1:277–285Google Scholar
  6. Becker N (2008) Influence of climate change on mosquito development and mosquito-borne diseases in Europe. Parasitol Res 103:S19–S28PubMedCrossRefGoogle Scholar
  7. Becker N, Jöst A, Weitzel T (2012) The Culex pipiens complex in Europe. J Am Mosq Control Assoc 28(4 Suppl):53–67PubMedCrossRefGoogle Scholar
  8. Becker N, Zqomba M, Petric D, Dahl C, Boase C, Lane J, Kaiser A (2003) Mosquitoes and their control, 1st edn. Kluwer Academic/ Plenum Publishers, New YorkCrossRefGoogle Scholar
  9. Bernard KA, Maffei JG, Jones SA, Kauffman EB, Ebel G, Dupuis AP II, Ngo KA, Nicholas DC, Young DM, Shi PY, Kulasekera VL, Eidson M, White DJ, Stone WB, Kramer LD (2001) West Nile Virus infection in birds and mosquitoes, New York State, 2000. Emerg Infect Dis 7:679–685PubMedCrossRefPubMedCentralGoogle Scholar
  10. Bolling BG, Barker CM, Moore CG, Pape WJ, Eisen L (2009) Seasonal patterns for entomological measures of risk for exposure to Culex vectors and West Nile Virus in relation to human disease cases in Northeastern Colorado. J Med Entomol 46:1519–1531PubMedCrossRefPubMedCentralGoogle Scholar
  11. Calado DC, Navarro-Silva MA (2002) Influência da temperatura sobre a longevidade, fecundidade e atividade hematofágica de Aedes (Stegomyia) albopictus Skuse, 1894 (Diptera, Culicidae) sob condições de laboratório. Rev Bras Entomol 46:93–98, English summaryCrossRefGoogle Scholar
  12. Chambers JM, Hastie TJ (1992) Statistical models in S, 1st edn. Wadsworth & Brooks/Cole, Pacific Grove, CAGoogle Scholar
  13. Ciota AT, Matacchiero AC, Marm Kilpatrick A, Kramer LD (2014) The effect of temperature on life history traits of Culex mosquitoes. J Med Entomol 51:55–62PubMedCrossRefGoogle Scholar
  14. Ciota AT, Chin PA, Kramer LD (2013) The effect of hybridization of Culex pipiens complex mosquitoes on transmission of West Nile virus. Parasit Vectors. doi: 10.1186/1756–3305–6–305 PubMedPubMedCentralGoogle Scholar
  15. Cossins AR, Bowler K (1987) Temperature biology of animals, 1st edn. Chapman and Hall, New YorkCrossRefGoogle Scholar
  16. Davidowitz G, Nijhout HF (2004) The physiological basis of reaction norms: the interaction among growth rate, the duration of growth and body size. Integr Comp Biol 44:443–449PubMedCrossRefGoogle Scholar
  17. Farajollahi A, Fonseca DM, Kramer LD, Marm KA (2011) “Bird biting” mosquitoes and human disease: a review of the role of Culex pipiens complex mosquitoes in epidemiology. Infect Genet Evol 11:1577–1585PubMedCrossRefPubMedCentralGoogle Scholar
  18. Fonseca DM, Keyghobadi N, Malcolm CA, Mehmet C, Schaffner F, Mogi M, Fleischer RC, Wilkerson RC (2004) Emerging vectors in the Culex pipiens complex. Science 303:1535–1538PubMedCrossRefGoogle Scholar
  19. Hallmann GJ, Denlinger DL (1998) Introduction: temperature sensitivity and integrated pest management. In: Hallman GJ, Denlinger DL (eds) Temperature sensitivity in insects and application in integrated pest management. Westview Press, Boulder, CO, pp 1–5Google Scholar
  20. Hayes EB, Komar N, Nasci RS, Montgomery SP, O’Leary DR, Campbell GL (2005) Epidemiology and transmission dynamics of West Nile Virus disease. Emerg Infect Dis 11:1167–1173PubMedCrossRefPubMedCentralGoogle Scholar
  21. Hubálek Z, Halouzka J (1999) West Nile fever—a reemerging mosquito-borne viral disease in Europe. Emerg Infect Dis 5:643–650PubMedCrossRefPubMedCentralGoogle Scholar
  22. Hubálek Z (2008) Mosquito-born viruses in Europe. Parasitol Res 103:S29–S43PubMedCrossRefGoogle Scholar
  23. Joshi DS (1996) Effect of fluctuating and constant temperatures on development, adult longevity and fecundity in the mosquito Aedes krombeini. J Thermal Biol 21:151–154CrossRefGoogle Scholar
  24. McGee CE, Shustov AV, Tsetsarkin K, Frolov IV, Mason PW, Vanlandingham DL, Higgs S (2010) Infection, dissemination, and transmission of a West Nile Virus green fluorescent protein infectious clone by Culex pipiens quinquefasciatus mosquitoes. Vector-Borne Zoonotic Dis 10:267–274PubMedCrossRefPubMedCentralGoogle Scholar
  25. Montgomery MJ, Thiemann T, Macedo P, Brown DA, Scott TW (2011) Blood-feeding patterns of the Culex pipiens complex in Sacramento and Yolo Counties, California. J Med Entomol 48:398–404PubMedCrossRefGoogle Scholar
  26. Nasci RS, Savage HM, White DJ, Miller JR, Cropp BC, Godsey MS, Kerst AJ, Bennett P, Gottfried K, Lanciotti RS (2001) West Nile virus in overwintering Culex mosquitoes, New York City, 2000. Emerg Infect Dis 7:742–744PubMedCrossRefPubMedCentralGoogle Scholar
  27. Nelms BM, Macedo PA, Kothera L, Savage HM, Reisen WK (2013) Overwintering biology of Culex (Diptera: Culicidae) mosquitoes in the Sacramento valley of California. J Med Entomol 50:773–790PubMedCrossRefPubMedCentralGoogle Scholar
  28. Osório HC, Zézé L, Amaro F, Nunes A, Alves MJ (2014) Sympatric occurrence of Culex pipiens (Diptera, Culicidae) biotypes pipiens, molestus and their hybrids in Portugal, Western Europe: feeding patterns and habitat determinants. Med Vet Entomol 28:103–109PubMedCrossRefGoogle Scholar
  29. Paaijmans KP, Thomas MB (2011) The influence of mosquito resting behaviour and associated microclimate for malaria risk. Malar J 10:183. doi: 10.1186/1475–2875–10–183 PubMedCrossRefPubMedCentralGoogle Scholar
  30. Platonov AE, Fedorova MV, Karan LS, Shopenskaya TA, Platonova OV, Zhuravlev VI (2008) Epidemiology of West Nile infection in Volgograd, Russia, in relation to climate change and mosquito (Diptera: Culicidae) bionomics. Parasitol Res 103:S45–S53PubMedCrossRefGoogle Scholar
  31. R Core Team (2012) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3–900051–07–0, URL
  32. Reimann CA, Hayes EB, DiGuiseppi C, Hoffman R, Lehman JA, Lindsey NP, Campbell GL, Fischer M (2008) Epidemiology of neuroinvasive arboviral disease in the United States, 1999–2007. Am J Trop Med Hyg 79:974–979PubMedGoogle Scholar
  33. Savopoulou-Soultani M, Andreadis SS, Soultani-Zouroulidi CV (2011) Insects and arthropods of medical importance. CopyCity Publishing, Thessaloniki, Greece (in Greek)Google Scholar
  34. Shapiro H, Micucci S (2003) Pesticide use for West Nile virus. Can Med Assoc J 168:1427–1430Google Scholar
  35. Shaikevich EV, Vinogradova EB (2014) The discovery of a hybrid population of mosquitoes of the Culex pipiens L. complex (Diptera, Culicidae) on the Kos Island (Greece) by means of molecular markers. Entomol Rev 94:35–39CrossRefGoogle Scholar
  36. Smith JL, Fonseca DM (2004) Rapid assays for identification of members of the Culex (Culex) pipiens complex, their hybrids, and other sibling species (Diptera: Culicidae). Am J Trop Med Hyg 70:339–345PubMedGoogle Scholar
  37. Snow KR (1990) Mosquitoes, naturalists’ handbooks 14. Richmond Publishing Ltd., Slough, UKGoogle Scholar
  38. Syed Z, Leal WS (2009) Leal Acute olfactory response of Culex mosquitoes to a human and bird-derived attractant. Proc Natl Acad Sci U S A 106:18803–18808PubMedCrossRefPubMedCentralGoogle Scholar
  39. Weitzel T, Collado A, Jöst A, Pietsch K, Storch V, Becker N (2009) Genetic differentiation of populations within the Culex pipiens complex and phylogeny of related species. J Am Mosq Control Assoc 25:6–17PubMedCrossRefGoogle Scholar
  40. Zeller H, Lenglet A, Van Bortel W (2010) West Nile virus: the need to strengthen preparedness in Europe. Euro Surveill 15(34):19647PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • S. S. Andreadis
    • 1
    • 2
    Email author
  • O. C. Dimotsiou
    • 1
  • M. Savopoulou-Soultani
    • 1
  1. 1.Laboratory of Applied Zoology and Parasitology, Department of Plant Protection, Faculty of AgricultureAristotle University of ThessalonikiThessalonikiGreece
  2. 2.Chemical Ecology Lab, Department of EntomologyPenn State UniversityPennsylvaniaUSA

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