Parasitology Research

, Volume 113, Issue 11, pp 4023–4032 | Cite as

Clamp ultrastructure of the basal monogenean Chimaericola leptogaster (Leuckart, 1830) (Polyopisthocotylea: Chimaericolidae)

  • Larisa G. Poddubnaya
  • Willy Hemmingsen
  • David I. Gibson
Original Paper

Abstract

The ultrastructure of the haptoral clamps of the chimaericolid monogenean Chimaericola leptogaster, a basal polyopisthocotylean from the gills of a holocephalan fish, is described. These clamps are characterized by the presence of two muscle blocks interrupted mid-anteriorly and mid-posteriorly and different kinds of hard structures: a single median and paired lateral sclerites embedded in the clamp wall; six spine-like structures directed towards the clamp lumen; and electron dense surface structures along the internal surface of the anterior clamp lips and along the luminal surface of the tegument of the clamp lumen. The lateral sclerites are situated deep within muscular tissue and are closely bounded by radial myofibrils, possessing a uniform electron dense matrix within which are hollow areas of different sizes. The median sclerite occupies an area between the clamp wall myofibrils and the luminal epithelium, is surrounded by a basement lamina and is composed of a heterogeneous matrix comprising two different morphological layers related to variations in the type and concentration of fibrils. Four of the spine-like structures are extensions of the margins of the two spindle-like muscle blocks in the clamps, i.e. the two anterior and two posterior structures, and the two others are situated at the lateral constrictions of the left and right muscle blocks. The electron dense surface structures are derivations of the clamp tegument or, to be more precise, its outer, densely fibrous region. These results are discussed in relation to the evidence that the haptoral clamps of C. leptogaster are apparently ancient origin.

Keywords

Monogenea Ultrastructure Haptoral clamps Relict fauna Chimaericola Hard structures 

References

  1. Boeger WA, Kritsky DC (1997) Coevolution of the Monogenoidea (Platyhelminthes) based on a revised hypothesis of parasite phylogeny. Int J Parasitol 27:1495–14511. doi:10.1016/S0020-7519(97)00140-9 PubMedCrossRefGoogle Scholar
  2. Boeger WA, Kritsky DC (2001) Phylogenetic relationships of the Monogenoidea. In: Littlewood DTJ, Bray RA (eds) Interrelationships of the Platyhelminthes. Taylor & Francis, London, pp 92–102Google Scholar
  3. Brinkmann A Jr (1942) On “Öctobothriumleptogaster F.S. Leuckart. Göteborgs Kungl. Vetenskaps och Vitterhets Samhälles Handlingar. Ser B 2:3–29Google Scholar
  4. Bychowsky BE (1957) Monogenetic trematodes, their systematics and phylogeny. Izdatel’stvo Academiya Nauk SSSR, Moscow, Russia. In RussianGoogle Scholar
  5. El-Naggar MM (1992) Ultrastructural observations on the marginal hooklets of the monogenean gill parasite Cichlidogyrus halli typicus. Int J Parasitol 22:613–619. doi:10.1016/0020-7519(92)90009-A PubMedCrossRefGoogle Scholar
  6. El-Naggar MM (1993) Ultrastructural observations on the hamuli of the monogenean gill parasite Cichlidogyrus halli typicus. Int J Parasitol 23:745–748. doi:10.1016/0020-7519(93)90070-F PubMedCrossRefGoogle Scholar
  7. Inoue JG, Miya M, Lam K, Tay BH, Danks JA, Bell J, Walker TI, Venkatesh B (2010) Evolutionary origin and phylogeny of the modern holocephalans (Chondrichthyes: Chimaeriformes): a mitogenomic perspective. Mol Biol Evol 27:2576–2586. doi:10.1093/molbev/msq147 PubMedCrossRefGoogle Scholar
  8. Jovelin R, Justine J-L (2001) Phylogenetic relationships within the polyopisthocotylean monogeneans (Platyhelminthes) inferred from partial 28S rDNA sequences. Int J Parasitol 31:393–401. doi:10.1016/S0020-7519(01)00114-X PubMedCrossRefGoogle Scholar
  9. Justine J-L, Rahmouni C, Gey D, Schoelinck C, Hoberg EP (2013) The monogenean which lost its clamps. PLoS One 8(11):e79155PubMedCrossRefPubMedCentralGoogle Scholar
  10. Llewellyn J (1965) The evolution of parasitic Platyhelminths. In: Taylor A (ed) Third symposium of the British society for parasitology. Blackwell Scientific Publications, Oxford, pp 47–78Google Scholar
  11. Lyons KM (1966) The chemical nature and evolutionary significance of monogenean attachment sclerites. Parasitology 56:63–100PubMedCrossRefGoogle Scholar
  12. Martens EE (1984) Ultrastructure of the spines in the copulatory organ of some Monocelididae (Turbellaria, Proseriata). Zoomorphology 104:261–265. doi:10.1007/BF00312007 CrossRefGoogle Scholar
  13. Olson PD, Littlewood DTJ (2002) Phylogenetics of the Monogenea—evidence from a medley of molecules. Int J Parasitol 32:233–244PubMedCrossRefGoogle Scholar
  14. Pascoe PL (1997) Monogenean parasites of deep-sea fishes from the rockall trough (N.E. Atlantic) including a new species. J Marine Biol Assoc UK 67:603–622. doi:10.1017/S0025315400027326 CrossRefGoogle Scholar
  15. Poddubnaya LG, Hemmingsen W, Gibson DI (2013) Ultrastructural characteristics of the vaginae of the basal monogenean Chimaericola leptogaster (Leuckart, 1830). Parasitol Res 112:1169–1177. doi:10.1007/s00436-013-3596-8 PubMedCrossRefGoogle Scholar
  16. Ramasamy P, Bhuvaneswari R (1993) The ultrastructure of the tegument and clamp attachment organ of Gotocotyla bivaginalis (Monogenea, Polyopisthocotylea). Int J Parasitol 23:213–220. doi:10.1016/0020-7519(93)90143-M PubMedCrossRefGoogle Scholar
  17. Ramasamy P, Hanna REB, Threadgold T (1986) The surface topography and ultrastructure of the tegument and haptor of Price multae (Monogenea). Int J Parasitol 16:581–589. doi:10.1016/0020-7519(86)90024-X CrossRefGoogle Scholar
  18. Shaw MK (1979a) The ultrastructure of the clamp wall of the monogenean gill parasite Gastrocotyle trachuri. Parasitol Res 58:243–258. doi:10.1007/BF00933931 Google Scholar
  19. Shaw MK (1979b) The ultrastructure of the clamp sclerites in Gastrocotyle trachuri and other clamp-bearing monogeneans. Parasitol Res 59:43–51. doi:10.1007/BF00927845 Google Scholar
  20. Shaw MK (1979c) The development of the clamp attachment organs of the monogenean Gastrocotyle trachuri. Parasitol Res 59:277–294. doi:10.1007/BF00927522 Google Scholar
  21. Shinn AP, Gibson DI, Sommerville C (1995) A study of the composition of the sclerites of Gyrodactylus Normann, 1932 (Monogenea) using X-ray elemental analysis. Int J Parasitol 25:797–805. doi:10.1016/0020-7519(95)00008-P PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Larisa G. Poddubnaya
    • 1
  • Willy Hemmingsen
    • 2
  • David I. Gibson
    • 3
  1. 1.I. D. Papanin Institute for Biology of Inland WatersRussian Academy of SciencesBorokRussia
  2. 2.Department of Arctic and Marine Biology, Faculty of Biosciences, Fisheries and EconomicsUniversity of TromsøTromsøNorway
  3. 3.Department of Life Sciences, Natural History MuseumLondonUK

Personalised recommendations