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Parasitology Research

, Volume 113, Issue 10, pp 3697–3701 | Cite as

Acaricide activity in vitro of Acmella oleracea against Rhipicephalus microplus

  • K. N. C. Castro
  • D. F. Lima
  • L. C. Vasconcelos
  • J. R. S. A. Leite
  • R. C. Santos
  • A. A. Paz Neto
  • L. M. Costa-Júnior
Original Paper

Abstract

Cattle tick control has been limited by the resistance of these parasites to synthetic acaricides. Natural products are a possible alternative as they have different mechanisms of action. Acmella oleracea is a native plant with a large cultivated area in the Amazon region and could be easily used for large-scale preparation of a commercial product. This study evaluated the in vitro action of the hexane extract of the aerial parts of A. oleracea on larvae and engorged females of the cattle tick Rhipicephalus microplus. Spilanthol was the major constituent with a content of 14.8 % in the extract. The hexane extract of A. oleracea was highly effective against larvae of R. microplus with an LC50 of 0.8 mg mL−1. Against engorged females, hexane extract of A. oleracea reduced oviposition and hatchability of eggs with an LC50 of 79.7 mg mL−1. Larvae and engorged females were killed by the hexane extract with high efficiency (>95 %) at concentrations of 3.1 and 150.0 mg mL−1, respectively. These results demonstrate that the hexane extract of A. oleracea has significant activity against R. microplus and has potential to be developed into formulations for tick control.

Keywords

Cattle Control Extract Tick 

Notes

Acknowledgments

The authors wish to thank João Batista Alves de Souza for his valuable contribution in the experiments.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • K. N. C. Castro
    • 1
  • D. F. Lima
    • 2
  • L. C. Vasconcelos
    • 2
  • J. R. S. A. Leite
    • 3
  • R. C. Santos
    • 3
  • A. A. Paz Neto
    • 4
  • L. M. Costa-Júnior
    • 5
  1. 1.Embrapa Meio-NorteParnaíbaBrazil
  2. 2.Anidro do Brasil Extrações S.A.ParnaíbaBrazil
  3. 3.Universidade Federal do PiauíParnaíbaBrazil
  4. 4.Universidade Estadual do PiauíParnaíbaBrazil
  5. 5.Universidade Federal do MaranhãoChapadinhaBrazil

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