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Parasitology Research

, Volume 113, Issue 8, pp 2797–2807 | Cite as

The apicomplexan parasite Eimeria arloingi induces caprine neutrophil extracellular traps

  • Liliana M. R. SilvaEmail author
  • Tamara Muñoz Caro
  • Rüdiger Gerstberger
  • Maria J. M. Vila-Viçosa
  • Helder C. E. Cortes
  • Carlos Hermosilla
  • Anja Taubert
Original Paper

Abstract

As a novel effector mechanism polymorphonuclear neutrophils (PMN) release neutrophil extracellular traps (NETs), which represent protein-labeled DNA matrices capable of extracellular trapping and killing of invasive pathogens. Here, we demonstrate for the first time NET formation performed by caprine PMN exposed to different stages (sporozoites and oocysts) of the goat apicomplexan protozoan parasite Eimeria arloingi. Scanning electron microscopy as well as fluorescence microscopy of sporozoites- and oocysts-PMN co-cultures revealed a fine network of DNA fibrils partially covering the parasites. Immunofluorescence analyses confirmed the co-localization of histones (H3), neutrophil elastase (NE), and myeloperoxidase (MPO) in extracellular traps released from caprine PMN. In addition, the enzymatic activity of NE was found significantly enhanced in sporozoite-exposed caprine PMN. The treatment of caprine NET structures with deoxyribonuclease (DNase) and the NADPH oxidase inhibitor diphenylene iodondium (DPI) significantly reduced NETosis confirming the classical characteristics of NETs. Caprine NETs efficiently trapped vital sporozoites of E. arloingi since 72 % of these stages were immobilized—but not killed—in NET structures. As a consequence, early infection rates were significantly reduced when PMN-pre-exposed sporozoites were allowed to infect adequate host cells. These findings suggest that NETs may play an important role in the early innate host response to E. arloingi infection in goats.

Keywords

Eimeria arloingi Apicomplexa NETs Goats Neutrophils 

Notes

Acknowledgments

We would like to thank Gerd Magdowski (Institute of Anatomy and Cell Biology, JLU Giessen, Germany) for his excellent assistance and kind help on SEM analyses. We further acknowledge Brigitte Hofmann, Christin Ritter, and Klaus Becker for their excellent technical assistance and blood collection. TMC is a Ph.D. student of the International Giessen Graduate Centre for the Life Sciences (GGL) at the JLU Giessen, Germany and LMRS is a Ph.D. student (grant BD/72032/2010) of the Foundation for Science and Technology (FCT). This work was partially funded by FEDER Funds through the Operational Program for Competitiveness Factors—COMPETE and National Funds through FCT under the Strategic Project PEst-C/AGR/UI0115/2011.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Liliana M. R. Silva
    • 1
    • 2
    Email author
  • Tamara Muñoz Caro
    • 2
  • Rüdiger Gerstberger
    • 3
  • Maria J. M. Vila-Viçosa
    • 1
  • Helder C. E. Cortes
    • 1
  • Carlos Hermosilla
    • 2
  • Anja Taubert
    • 2
  1. 1.ICAAM—Instituto Ciências Agrárias e Ambientais MediterrânicasIIFA/Universidade de ÉvoraÉvoraPortugal
  2. 2.Institute of Parasitology, BFSJustus Liebig University GiessenGiessenGermany
  3. 3.Institute of PhysiologyJustus Liebig University GiessenGiessenGermany

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