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Parasitology Research

, Volume 113, Issue 2, pp 761–767 | Cite as

Prevalence and diversity of Encephalitozoon spp. and Enterocytozoon bieneusi in wild boars (Sus scrofa) in Central Europe

  • Karel Němejc
  • Bohumil SakEmail author
  • Dana Květoňová
  • Vladimír Hanzal
  • Paweł Janiszewski
  • Pavel Forejtek
  • Dušan Rajský
  • Michaela Kotková
  • Petra Ravaszová
  • John McEvoy
  • Martin Kváč
Original Paper

Abstract

From 2011 to 2012, the occurrence of Enterocytozoon bieneusi and Encephalitozoon spp. was surveyed at 29 randomly selected localities (both forest areas and enclosures) across four Central European countries: Austria, the Czech Republic, Poland, and the Slovak Republic. Isolates were genotyped by PCR amplification and characterization of the internal transcribed spacer (ITS) region using Enterocytozoon and Encephalitozoon-specific protocols. PCR revealed 16 mono-infections of Encephalitozoon cuniculi, 33 mono-infections of Enterocytozoon bieneusi and 5 concurrent infections of both Encephalitozoon cuniculi and Enterocytozoon bieneusi out of 460 faecal samples. Two genotypes (I and II) were revealed by sequence analysis of the ITS region of Encephalitozoon cuniculi. Eleven genotypes, five previously found in other hosts including domestic pigs (D, EbpA, EbpC, G and Henan-I) and six novel (WildBoar1–6), were identified in Enterocytozoon bieneusi. No other microsporidia infection was found in the examined faecal samples. Prevalence of microsporidia at the locality level ranged from 0 to 58.8 %; the prevalence was less than 25 % at more than 86 % of localities. Enterocytozoon bieneusi was detected as a predominant species infecting Eurasian wild boars (Sus scrofa). The present report is the most comprehensive survey of microsporidia infections in wild boars within the Czech Republic and selected Central European countries.

Keywords

Internal Transcribe Spacer Faecal Sample Wild Boar Slovak Republic Central European Country 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by projects of Ministry of Education, Youth and Sports of the Czech Republic (MSM 6007665806), project of the Grant Agency of University of South Bohemia (022/2010/Z and 011/2013/Z), research project of the Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic (Z60220518).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Karel Němejc
    • 1
  • Bohumil Sak
    • 2
    Email author
  • Dana Květoňová
    • 2
  • Vladimír Hanzal
    • 1
    • 3
  • Paweł Janiszewski
    • 4
  • Pavel Forejtek
    • 5
  • Dušan Rajský
    • 6
  • Michaela Kotková
    • 2
  • Petra Ravaszová
    • 7
  • John McEvoy
    • 8
  • Martin Kváč
    • 1
    • 2
  1. 1.Faculty of AgricultureUniversity of South Bohemia in České BudějoviceČeské BudějoviceCzech Republic
  2. 2.Institute of ParasitologyBiology Centre of the Academy of Sciences of the Czech RepublicČeské BudějoviceCzech Republic
  3. 3.Faculty of Forestry and Wood SciencesCzech University of Life Sciences PraguePragueCzech Republic
  4. 4.Faculty of Animal BioengineeringUniversity of Warmia and MazuryOlsztynPoland
  5. 5.Institute for Wildlife EcologyUniversity of Veterinary and Pharmaceutical Sciences BrnoBrnoCzech Republic
  6. 6.Faculty of ForestryTechnical University in ZvolenZvolenSlovak Republic
  7. 7.Department of Biology and GeneticsUniversity of Veterinary Medicine and Pharmacy in KošiceKošiceSlovak Republic
  8. 8.Veterinary and Microbiological Sciences DepartmentNorth Dakota State UniversityFargoUSA

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