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Parasitology Research

, Volume 113, Issue 1, pp 275–283 | Cite as

Inhibitory effect of allicin on the growth of Babesia and Theileria equi parasites

  • Akram Ahmed Salama
  • Mahmoud AbouLaila
  • Mohamad Alaa Terkawi
  • Ahmed Mousa
  • Ahmed El-Sify
  • Mahmoud Allaam
  • Ahmed Zaghawa
  • Naoaki Yokoyama
  • Ikuo IgarashiEmail author
Original Paper

Abstract

Allicin is an active ingredient of garlic that has antibacterial, antifungal, antiviral, and antiprotozoal activity. However, the inhibitory effects of allicin on Babesia parasites have not yet been examined. In the present study, allicin was tested as a potent inhibitor against the in vitro growth of bovine and equine Babesia parasites and the in vivo growth of Babesia microti in a mouse model. The in vitro growth of Babesia bovis, Babesia bigemina, Babesia caballi, or Theileria equi was inhibited by allicin in a dose-dependent manner and had IC50 values of 818, 675, 470, and 742 μM, respectively. Moreover, allicin significantly inhibited (P < 0.001) invasion of B. bovis, B. bigemina, B. caballi, and T. equi into the host erythrocyte. Furthermore, mice treated with 30 mg/kg of allicin for 5 days significantly (P < 0.05) reduced the parasitemia of B. microti over the period of the study. To further examine the potential synergism of allicin with diminazene aceturate, growth inhibitory assays were performed in vitro and in vivo. Interestingly, combinations of diminazene aceturate with allicin synergistically potentiated its inhibitory effects in vitro and in vivo. These results indicate that allicin might be beneficial for the treatment of babesiosis, particularly when used in combination with diminazene aceturate.

Keywords

Triclosan Babesia Allicin Entamoeba Histolytica Growth Inhibition Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by the Ministry of Higher Education, Egypt; the Japanese Society for the Promotion of Science; and the Ministry of Education, Culture, Sports, Science, and Technology, Japan.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Akram Ahmed Salama
    • 1
    • 2
  • Mahmoud AbouLaila
    • 3
  • Mohamad Alaa Terkawi
    • 1
  • Ahmed Mousa
    • 1
  • Ahmed El-Sify
    • 2
  • Mahmoud Allaam
    • 2
  • Ahmed Zaghawa
    • 2
  • Naoaki Yokoyama
    • 1
  • Ikuo Igarashi
    • 1
    Email author
  1. 1.National Research Center for Protozoan DiseasesObihiro University of Agriculture and Veterinary MedicineObihiroJapan
  2. 2.Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary MedicineUniversity of Sadat CitySadat CityEgypt
  3. 3.Department of Parasitology, Faculty of Veterinary MedicineUniversity of Sadat CitySadat CityEgypt

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