Parasitology Research

, Volume 113, Issue 1, pp 217–222 | Cite as

Developmental rate of immatures of two fly species of forensic importance: Sarcophaga (Liopygia) ruficornis and Microcerella halli (Diptera: Sarcophagidae)

  • Mariana Prado Nassu
  • Patricia Jacqueline Thyssen
  • Aricio Xavier Linhares
Original Paper

Abstract

Since insect species are poikilothermic organisms, they generally exhibit different growth patterns depending on the temperature at which they develop. This factor is important in forensic entomology, especially for estimating postmortem interval (PMI) when it is based on the developmental time of the insects reared in decomposing bodies. This study aimed to estimate the rates of development, viability, and survival of immatures of Sarcophaga (Liopygia) ruficornis (Fabricius 1794) and Microcerella halli (Engel 1931) (Diptera: Sarcophagidae) reared in different temperatures: 10, 15, 20, 25, 30, and 35 ± 1 °C. Bovine raw ground meat was offered as food for all experimental groups, each consisting of four replicates, in the proportion of 2 g/larva. To measure the evolution of growth, ten specimens of each group were randomly chosen and weighed every 12 h, from initial feeding larva to pupae, and then discarded. Considering the records of weight gain, survival rates, and stability of growth rates, the range of optimum temperature for the development of S. (L.) ruficornis is between 20 and 35 °C, and that of M. halli is between 20 and 25 °C. For both species, the longest times of development were in the lowest temperatures. The survival rate at extreme temperatures (10 and 35 °C) was lower in both species. Biological data such as the ones obtained in this study are of great importance to achieve a more accurate estimate of the PMI.

Notes

Acknowledgments

We would like to thank Professor Dr. José R. P. Parra and Dra. Marinéia L. Haddad, from Escola Superior de Agricultura Luiz de Queiroz, State University of São Paulo (USP), for providing facilities in this research. This work was supported by the São Paulo State Foundation for the Support of Research (FAPESP), grant no. 2009/17523-0.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Mariana Prado Nassu
    • 1
  • Patricia Jacqueline Thyssen
    • 2
  • Aricio Xavier Linhares
    • 1
  1. 1.Department of Animal Biology, IBState University of Campinas (UNICAMP)CampinasBrazil
  2. 2.Department of Parasitology and Microbiology, IBFederal University of Pelotas (UFPEL)PelotasBrazil

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