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Parasitology Research

, Volume 113, Issue 1, pp 121–129 | Cite as

Molecular characterization of voltage-gated calcium channel β-subunits of Clonorchis sinensis

  • Pyo Yun Cho
  • Won Gi Yoo
  • Tae Im Kim
  • Seong Kyu Ahn
  • Shin-Hyeong Cho
  • Tong-Soo Kim
  • Sung-Jong HongEmail author
Original Paper

Abstract

The voltage-gated Ca2+ channel β-subunit is a member of the membrane-associated guanylate kinase family and modulates kinetic properties of the Ca2+ channels, such as their voltage-dependent activation and inactivation rates. Two cDNA clones were identified to encode each β-subunit isotype of the voltage-gated Ca2+ channel of Clonorchis sinensis, CsCavβ1 and CsCavβ2, which consist of 606 and 887 amino acids, respectively. CsCavβ1 was found to be similar to the β-subunit containing two conserved serine residues that constitute the consensus protein kinase C phosphorylation site in the β-interaction domain (BID). CsCavβ2 had cysteine and alanine residues instead of the two serine residues conserved in BID and was homologous to variant β-subunit of Schistosoma mansoni and Schistosoma japonicum. CsCavβ1 and CsCavβ2 were almost equally expressed in the adults and metacercariae, but were more expressed in adult C. sinensis than in metacercariae. Collectively, our findings suggest that substitution of the two serine residues in BID of CsCavβ2 may render C. sinensis sensitive to praziquantel.

Keywords

Adult Worm Serine Residue Praziquantel Schistosoma Japonicum Alanine Residue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by a grant (NIH-4847-302-210-13) from the National Institute of Health, Center for Disease Control and Prevention, Ministry of Health and Welfare, Republic of Korea.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Pyo Yun Cho
    • 1
  • Won Gi Yoo
    • 2
  • Tae Im Kim
    • 3
  • Seong Kyu Ahn
    • 1
  • Shin-Hyeong Cho
    • 2
  • Tong-Soo Kim
    • 1
  • Sung-Jong Hong
    • 3
    Email author
  1. 1.Department of Parasitology and Inha Research Institute for Medical SciencesInha University School of MedicineIncheonRepublic of Korea
  2. 2.Division of Malaria and Parasitic Diseases, National Institute of HealthKorea Center for Disease Control and PreventionOsongRepublic of Korea
  3. 3.Department of Medical Environmental BiologyChung-Ang University College of MedicineSeoulRepublic of Korea

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