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Parasitology Research

, Volume 112, Issue 11, pp 3883–3886 | Cite as

Rapid detection and identification of Theileria equi and Babesia caballi by high-resolution melting (HRM) analysis

  • Bashir SalimEmail author
  • Mohammed Ahmed Bakheit
  • Chihiro Sugimoto
Original Paper

Abstract

The application of high-resolution melting (HRM) analysis in the differentiation between Theileria equi and Babesia caballi was evaluated using control samples from the United States Department of Agriculture and field samples collected from horses in Sudan and China. A region of the 18S rRNA gene, with four known nucleotide differences between the two parasites, was selected for primer design. HRM analysis successfully allowed the detection and differentiation of T. equi and B. caballi without the necessity of performing time-consuming and expensive post-PCR procedures such as sequencing or restriction digestion. Our results suggest that HRM could be an ideal method for rapid genotyping, which is required to determine a drug of choice or to administer an appropriate vaccine during an outbreak.

Keywords

Babesia Melting Profile Reverse Line Blotting Babesia Species Difference Graph 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by Grant-in-Aid for JSPS fellows and for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT); the program of the Funding Research Center for Emerging and Re-emerging Infectious Disease, MEXT; and Asia-Africa S & T Strategic Cooperation Promotion Program by the Special Coordination Funds for Promoting Science & Technology, MEXT. We would like to thank Mr. Ryo Nakao for his assistance.

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Bashir Salim
    • 1
    • 2
    Email author
  • Mohammed Ahmed Bakheit
    • 1
    • 3
  • Chihiro Sugimoto
    • 4
  1. 1.Department of Parasitology, Faculty of Veterinary MedicineUniversity of KhartoumKhartoum NorthSudan
  2. 2.School of Biology, Centre for Genetics and GenomicsThe University of NottinghamNottinghamUK
  3. 3.Veterinary Infection Biology and ImmunologyResearch Center BorstelBorstelGermany
  4. 4.Department of Collaboration and Education, Research Center for Zoonosis ControlHokkaido UniversitySapporoJapan

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