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Parasitology Research

, Volume 112, Issue 9, pp 3283–3288 | Cite as

Alteration in Bacillus thuringiensis toxicity by curing gut flora: novel approach for mosquito resistance management

  • Chandrashekhar D. Patil
  • Hemant P. Borase
  • Bipinchandra K. Salunke
  • Satish V. PatilEmail author
Original Paper

Abstract

Mosquitoes are known for acquiring resistance against insecticides in many ways, namely target side mutation, enzyme modification, sequestration, quick elimination, etc. But, the role of microflora present in abundance in the larval midgut is less explored with respect to their role in insecticide resistance. During the course of their development, mosquitoes are continuously exposed to microbes and have naturally acquired midgut microbial flora. This midgut flora can modulate the mosquito's susceptibility to Bacillus thuringiensis (Bt) infection by degrading toxic Bt protein forms through an unknown mechanism. In this study, we show that microbe-free aseptic mosquito larvae displayed an increased susceptibility to Bt toxicity compared to larvae harboring natural microbial flora. Fourth instar larvae of Anopheles stephensi were treated separately with penicillin, streptomycin, erythromycin (100 μg/ml), and mixtures of all three antibiotics and then analyzed for Bt toxicity. We have also examined the influence of the mosquito's midgut microbial flora under microaerophilic condition on the Bt protein degradation through plate, broth, TLC, and UV–vis spectrophotometric assay. A better understanding of the roles of microbiota in preventing Bt toxicity to mosquitoes could potentially lead to the development of new sustainable mosquito control strategies.

Keywords

Mosquito Larva Larvicidal Activity Anopheles Stephensi Microaerophilic Condition Mosquito Midgut 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Chandrashekhar D. Patil is thankful to CSIR (09/728 (0028)/2012-EMR-I) for the award of senior research fellowship.

Supplementary material

436_2013_3507_MOESM1_ESM.docx (111 kb)
ESM 1 (DOCX 111 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Chandrashekhar D. Patil
    • 1
  • Hemant P. Borase
    • 1
  • Bipinchandra K. Salunke
    • 1
  • Satish V. Patil
    • 1
    • 2
    Email author
  1. 1.School of Life SciencesNorth Maharashtra UniversityJalgaonIndia
  2. 2.North Maharashtra Microbial Culture Collection Centre (NMCC)North Maharashtra UniversityJalgaonIndia

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