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Parasitology Research

, Volume 112, Issue 2, pp 829–838 | Cite as

Candidatus Mesochlamydia elodeae” (Chlamydiae: Parachlamydiaceae), a novel chlamydia parasite of free-living amoebae

  • Daniele Corsaro
  • Karl-Dieter Müller
  • Jost Wingender
  • Rolf Michel
Original Paper

Abstract

Vannella sp. isolated from waterweed Elodea sp. was found infected by a chlamydia-like organism. This organism behaves like a parasite, causing the death through burst of its host. Once the vannellae degenerated, the parasite was successfully kept in laboratory within a Saccamoeba sp. isolated from the same waterweed sample, which revealed in fine through electron microscopy to harbor two bacterial endosymbionts: the chlamydial parasite we introduce and another endosymbiont initially and naturally present in the host. Herein, we provide molecular-based identification of both the amoeba host and its two endosymbionts, with special focus on the chlamydia parasite. High sequence similarity values of the 18S rDNA permitted to assign the amoeba to the species Saccamoeba lacustris (Amoebozoa, Tubulinea). The bacterial endosymbiont naturally harbored by the host belonged to Sphingomonas koreensis (Alpha-Proteobacteria). The chlamydial parasite showed a strict specificity for Saccamoeba spp., being unable to infect a variety of other amoebae, including Acanthamoeba, and it was itself infected by a bacteriophage. Sequence similarity values of the 16S rDNA and phylogenetic analysis indicated that this strain is a new member of the family Parachlamydiaceae, for which we propose the name “Candidatus Mesochlamydia elodeae.”

Keywords

Testate Amoeba Limax Infected Amoeba Acanthamoeba Strain Amoeba Host 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Elke Schneider and Liane Junglas from the Electron Microscopy Department for the excellent technical assistance (Head of the department: Dr. Bärbel Hauröder). DC was partially supported by Novartis Foundation.

Supplementary material

436_2012_3213_Fig6_ESM.jpg (34 kb)
Supplementary Figure 1

In situ identification of Mesochlamydia strain KV in Saccamoeba lacustris cells by FISH using Cy3-labeled probe Chls‐523, which targets all Chlamydiae (red) (Poppert et al. 2002). 1000×. (JPEG 34 kb)

436_2012_3213_MOESM1_ESM.tif (732 kb)
High resolution image (TIFF 731 kb)
436_2012_3213_MOESM2_ESM.xls (32 kb)
Supplementary Table 1 Pair-wise sequence similarity values for near full 16S rDNA of major representing taxa of Chlamydiae (XLS 32 kb)
436_2012_3213_MOESM3_ESM.xls (22 kb)
Supplementary Table 2 Pair-wise sequence similarity values within the family Parachlamydiaceae (XLS 21 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Daniele Corsaro
    • 1
    • 2
  • Karl-Dieter Müller
    • 3
  • Jost Wingender
    • 4
  • Rolf Michel
    • 5
  1. 1.Chlamydia Research Association (CHLAREAS)Vandoeuvre-lès-NancyFrance
  2. 2.Laboratory of Soil Biology, Institute of BiologyUniversity of NeuchâtelNeuchâtelSwitzerland
  3. 3.Institut für Medizinische Mikrobiologie der Universität Duisburg-EssenEssenGermany
  4. 4.Biofilm Centre, Aquatic MicrobiologyUniversity of Duisburg-EssenEssenGermany
  5. 5.Central Institute of the Federal Armed Forces Medical ServicesKoblenzGermany

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