Parasitology Research

, Volume 112, Issue 3, pp 1357–1361 | Cite as

Evidences of increasing risk of dirofilarioses in southern Italy

  • A. GiangasperoEmail author
  • M. Marangi
  • M. S. Latrofa
  • D. Martinelli
  • D. Traversa
  • D. Otranto
  • C. Genchi
Short Communication


Given the spread of Aedes albopictus from northern to southern Italy, and the lack of updated data on Dirofilaria infections, this study was carried out to assess the infection risk for dogs and cats in Apulia region. During a 2-year study, 175 A. albopictus female specimens and samples of blood from 427 dogs (309 privately owned dogs and 118 shelter dogs) and 12 cats were collected. All blood samples were subjected to a modified Knott method, to a test for the detection of circulating Dirofilaria immitis antigen, and to a Dirofilaria species-specific real-time PCR for the simultaneous detection of D. immitis and Dirofilaria repens, targeting on partial cytochrome oxidase subunit 1 and internal transcribed spacer-2, respectively. Two abdomen and one thorax pools from A. albopictus were positive for D. immitis, with minimum infection rates of 1.14 and 0.51, respectively, and a probability of a single positive specimen to be infected of P = 0.6 % (95 % confidence interval (CI) = 0.12–1.73). Out of 439 examined subjects, 22 (5.0 %) tested positive for Dirofilaria spp. in at least one diagnostic test. A specific D. immitis infestation rate of 3.5 % was found among the privately owned dogs, while shelter dogs tested positive only for D. repens with a prevalence of 3.4 %; one cat tested molecularly positive for D. immitis. There was a significantly higher rate of positivity among guard dogs for D. immitis (odds ratio, 6.24, 95 % CI, 1.26–25.28; P < 0.05). The increasing risk of D. immitis infection in southern Italy is supported by the noteworthy positivity of A. albopictus populations and the cat. Our data highlight the usefulness to include filarioid infestation in routine diagnosis.


Apulia Region Heartworm Disease Minimum Infection Rate Heartworm Antigen Dirofilaria Infection 
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The authors are grateful to all the veterinarians who collaborated so generously with this study: Sergio Spirito, Antonio Latino, Mauro Saggese, Angelo Marinelli, Raffaele Lellis, Vittoria Piracci, Stefania Luprano, Ruben Leccese, Antonia Mangano, Donato Raele, Eleonora Fonseca, Maria Rosaria Saponaro, Giovanni Normanno, Sandra Di Macchia, Rossana Placentino, Stefania Luprano, Elisabetta De Maio, and Morena Taraschi. The authors also thank Antonio Narducci, Donato Raele, and Raffaella Terlizzi for their valuable work in the field and in the lab. This work was funded by the Fondazione della Banca del Monte, Foggia, Italy (2010–2011).


  1. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402PubMedCrossRefGoogle Scholar
  2. Caminade C, Medlock JM, Ducheyne E, McIntyre KM, Leach S, Baylis M, Morse AP (2012) Suitability of European climate for the Asian tiger mosquito Aedes albopictus: recent trends and future scenarios. J R Soc Interface. doi: 10.1098/rsif.2012.0138
  3. Cancrini G, Frangipane di Regalbono A, Ricci I, Tessarin C, Gabrielli S, Pietrobelli M (2003) Aedes albopictus is a natural vector of Dirofilaria immitis in Italy. Vet Parasitol 118:195–202PubMedCrossRefGoogle Scholar
  4. Cancrini G, Scaramozzino P, Gabrielli S, Di Paolo M, Toma L, Romi R (2007) Aedes albopictus and Culex pipiens implicated as natural vectors of Dirofilaria repens in central Italy. J Med Entomol 44:1064–1066PubMedCrossRefGoogle Scholar
  5. Cinco M, Padovan D, Murgia R, Frusteri L, Maroli M, Van de Pol I, Verbeek-De Kruif N, Rijpkema S, Taggi F (1998) Prevalence of Borrelia burgdorferi infection in Ixodes ricinus in central Italy. Eur J Clin Microbiol Infect Dis 17:134–135PubMedGoogle Scholar
  6. Cringoli G, Rinaldi L, Veneziano V, Capelli G (2001) A prevalence survey and risk analysis of filariosis in dogs from the Mt. Vesuvius area of southern Italy. Vet Parasitol 102:243–252PubMedCrossRefGoogle Scholar
  7. Di Cesare A, Castagna G, Meloni S, Milillo P, Latrofa S, Otranto D, Traversa D (2011) Canine and feline infections by cardiopulmonary nematodes in central and southern Italy. Parasitol Res 109(Suppl 1):S87–S96PubMedCrossRefGoogle Scholar
  8. Genchi C, Kramer L, Rivasi F (2011) Dirofilarial infection in Europe. Vector Borne Zoonotic Dis 11:1307–1317PubMedCrossRefGoogle Scholar
  9. Genchi C, Rinaldi L, Cascone C, Mortarino M, Cringoli G (2005) Is heartworm really spreading in Europe? Vet Parasitol 133:137–148PubMedCrossRefGoogle Scholar
  10. Genchi C, Rinaldi L, Genchi M (2010) Impact of climate change on Dirofilaria infections in animals and humans. Nuova Acta Leopoldina NF 111:71–77Google Scholar
  11. Genchi C, Venco L, Ferrari N, Mortarino M, Genchi M (2008) Feline heartworm (Dirofilaria immitis) infection: a statistical elaboration of the duration of the infection and life expectancy in asymptomatic cats. Vet Parasitol 158:177–182PubMedCrossRefGoogle Scholar
  12. Giangaspero A (2011) Ricerche in Puglia. La Zanzara Tigre: passato, presente e futuro. Rapporto scientifico per la Fondazione Banca del Monte. 19 Ottobre 2011, Foggia, Italy (In Italian)Google Scholar
  13. Kartashev V, Batashova I, Kartashov S, Ermakov A, Mironova A, Kuleshova Y, Ilyasov B, Kolodiy I, Klyuchnikov A, Ryabikina E, Babicheva M, Levchenko Y, Pavlova R, Pantchev N, Morchón R, Simón F (2011) Canine and human dirofilariosis in the Rostov Region (Southern Russia). Vet Med Int 11:685–713Google Scholar
  14. Latrofa MS, Montarsi F, Ciocchetta S, Annoscia G, Dantas-Torres F, Ravagnan S, Capelli G, Otranto D (2012) Molecular xenomonitoring of Dirofilaria immitis and Dirofilaria repens in mosquitoes from north-eastern Italy by real-time PCR coupled with melting curve analysis. Parasite Vector 20:5–76. doi: 10.1186/1756-3305-5-76 Google Scholar
  15. Mandelli G, Mantovani A (1966) Su di un caso di infestazione massiva da Dirofilaria repens nel cane. Parassitologia 8:21–28Google Scholar
  16. McCall JW, Genchi C, Kramer LH, Guerrero J, Venco L (2008) Heartworm disease in animals and humans. Adv Parasitol 66:193–285PubMedCrossRefGoogle Scholar
  17. Otranto D, Capelli G, Genchi C (2009) Changing distribution patterns of canine vector borne diseases in Italy: leishmaniosis vs. dirofilariosis. Parasite Vector 26(2 Suppl):1–S2. doi: 10.1186/1756-3305-2-S1-S2 Google Scholar
  18. Otranto D, Eberhard ML (2011) Zoonotic helminths affecting the human eye. Parasite Vector 23:4–41. doi: 10.1186/1756-3305-4-41 Google Scholar
  19. Otranto D, Lia RP, Testini G, Britti D, Puntorieri EP, Genchi C (2008) Authocthonous cases of canine dirofilariosis in Southern Italy. Parassitologia 50:226Google Scholar
  20. Pampiglione S, Rivasi F (2000) Human dirofilariasis due to Dirofilaria (Nochtiella) repens: an update of world literature from 1995 to 2000. Parassitologia 42:231–254PubMedGoogle Scholar
  21. Piergili Fioretti D, Diaferia M, Grelloni V, Maresca G (2003) Canine filariosis in Umbria: an update of the occurrence one year after the first observation of autochtonous foci. Parassitologia 45:79–83PubMedGoogle Scholar
  22. Puccini V, Abbenante MG (1980) Dirofilariosi del cane in Puglia: indagine epizoologica. Atti SisVet 34:323Google Scholar
  23. Rocconi F, Di Tommaso M, Traversa D, Palmieri C, Pampurini F, Boari A (2012) Allergic dermatitis by Dirofilaria repens in a dog: clinical picture and treatment. Parasitol Res 111:493–496PubMedCrossRefGoogle Scholar
  24. Sassnau R, Dyacenko V, Pantchev N, Stöckel F, Dittmar K, Daugschies A (2009) Dirofilaria repens Befall in einenm Schlittenhunde-Rudel im Land Brandenburg. Tierärztl Prax 37:95–101Google Scholar
  25. Scaramozzino P, Gabrielli S, Di Paolo M, Sala M, Scholl F, Cancrini G (2005) Dog filariosis in the Lazio region (Central Italy): first report on the presence of Dirofilaria repens. BMC Infect Dis 26:5–75Google Scholar
  26. Severini F, Toma L, Di Luca M, Romi R (2009) Le zanzare italiane: generalità e identificazione degli adulti (Diptera, Culicidae). Fragmenta Entomol 41:213–372Google Scholar
  27. Venco L, Genchi M, Genchi C, Gatti D, Kramer L (2011) Can hearthworm prevalence in dogs be used as provisional data for assessing the prevalence of the infection in cats? Vet Parasitol 176:300–303PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • A. Giangaspero
    • 1
    Email author
  • M. Marangi
    • 1
  • M. S. Latrofa
    • 2
  • D. Martinelli
    • 3
  • D. Traversa
    • 4
  • D. Otranto
    • 2
  • C. Genchi
    • 5
  1. 1.Dipartimento di Scienze Agrarie, degli Alimenti e dell’AmbienteUniversità degli Studi di FoggiaFoggiaItaly
  2. 2.Dipartimento di Medicina veterinariaUniversità di BariBariItaly
  3. 3.Dipartimento di Scienze Mediche e ChirurgicheUniversità di FoggiaFoggiaItaly
  4. 4.Dipartimento di Scienze Biomediche ComparateUniversità di TeramoTeramoItaly
  5. 5.Dipartimento di Scienze veterinarie e Sanità pubblicaUniversità di MilanoMilanItaly

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