Parasitology Research

, Volume 111, Issue 6, pp 2295–2299 | Cite as

Effect of Ageratum houstonianum Mill. (Asteraceae) leaf extracts on the oviposition activity of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)

  • Samuel TennysonEmail author
  • K. John Ravindran
  • Alex Eapen
  • S. John William
Original Paper


Plant extracts have been studied extensively for their insecticidal activity against immature stages and adult mosquitoes. They have also been reported to influence the habitat preference of ovipositing mosquitoes. Ageratum houstonianum, a medicinal plant belonging to the family Asteraceae, has been reported to possess insecticidal activity, and in the present study, the ovipositional attractant/deterrent activity was studied. The effect of Ageratum houstonianum crude leaf extracts on the oviposition of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus was studied at 0.1 % concentration in laboratory. Among hexane, ethyl acetate and methanol crude leaf extracts, methanol showed an effective deterrent activity against all the three vector species with an oviposition active index of −0.8, −0.8 and −0.9, respectively. Field trials carried out to study the effect of 0.1 % methanol extract on oviposition of Aedes species indicated effective deterrence ranging from 79.0 to 100.0 % in indoor and 74.6 to 100.0 % in outdoor ovitraps. The potential oviposition deterrent property of Ageratum houstonianum crude leaf extracts observed in both laboratory and field studies indicates the presence of phytocompounds that act as effective contact deterrent. Further, isolation, identification and preparation of suitable formulation of the effective phytocompounds of Ageratum houstonianum that act as a contact deterrent are required.


Leaf Extract Vector Mosquito Anopheles Stephensi Aedes Aegypti Oviposition Deterrent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Authors are thankful to the staff of the National Institute of Malaria Research (ICMR), Field Unit, Chennai, Tamil Nadu, India for their kind assistance.


  1. Angerilli NPD (1980) Influences of extracts of freshwater vegetation on the survival and oviposition by Aedes aegypti. Can Entomol 112:1249–1252CrossRefGoogle Scholar
  2. Arivoli S, Samuel T (2011) Larvicidal efficacy of Cleistanthus collinus (Roxb.) (Euphorbiaceae) leaf extracts against vector mosquitoes (Diptera: Culicidae). Asian Pac J. Trop Biomed 1(2):S275–S277Google Scholar
  3. Arivoli S, Ravindran J, Samuel T (2012) Larvicidal efficacy of plant extracts against the malarial vector Anopheles stephensi Liston (Diptera: Culicidae). World J Med Sci 7(2):77–80Google Scholar
  4. Beehler JW, Millar JG, Mulla MS (1994) Field evaluation of synthetic compounds mediating oviposition in Culex mosquitoes (Diptera: Culicidae). J Chem Ecol 20:281–291CrossRefGoogle Scholar
  5. Bentley MD, Day JF (1989) Chemical ecology and behavioural aspects of mosquito oviposition. Annu Rev Entomol 34:401–421PubMedCrossRefGoogle Scholar
  6. Bowers WS, Ohta T, Cleeve JS (1976) Discovery of insect antijuvenile hormones in plants. Science 193:542–547Google Scholar
  7. Choochote W, Chaithong U, Kamsuk K, Rattanachanpichai E, Jitpakdi A, Tippawangkosol P, Chaiyasit D, Champakaew D, Tuetun B, Pitasawat B (2006) Adulticidal activity against Stegomyia aegypti (Diptera: Culicidae) of three Piper spp. Rev Inst Med Trop Sao Paulo 48(1):33–37PubMedCrossRefGoogle Scholar
  8. Elango G, Rahuman AA, Bagwan A, Kamraj C, Zahir AA, Rajakumar G, Marimuthu S, Santhoshkumar T (2010) Efficacy of botanical extracts against Japanese encephalitis vector, Culex tritaeniorhynchus. Parasitol Res 106:481–492PubMedCrossRefGoogle Scholar
  9. Ikeshoji T, Saito K, Yano A (1975) Bacterial production of the ovipositional attractants for mosquitoes on fatty acid substrates. Appl Entomol Zool 10:239–242Google Scholar
  10. Jeyabalan D, Arul N, Thangamathi P (2003) Studies on the effects of Pelargonium citrosa leaf extracts on malaria vector, Anopheles stephensi Liston. Biores Technol 89:185–189CrossRefGoogle Scholar
  11. Johnson MF (1971) A monograph of the genus Ageratum L. (Compositae-Eupatorieae). Ann Mo Bot Gard 58:6–88CrossRefGoogle Scholar
  12. Judd JGR, Borden JH (1980) Oviposition deterrents for Aedes aegypti in extracts of Lemna minor. J Entomol Soc 17:30–33Google Scholar
  13. Kramer WL, Mulla S (1979) Oviposition attractants and repellents of mosquitoes: oviposition responses of Culex mosquitoes to organic infusions. Environ Entomol 8:1111–1117Google Scholar
  14. Mboera LEG, Midra KY, Salum FM, Takken W, Pickett JA (1999) The influence of synthetic oviposition pheromone and volatiles from soakage pits and grass infusions upon oviposition site selection of Culex mosquitoes in Tanzania. J Chem Ecol 25:1855–1865CrossRefGoogle Scholar
  15. Millar JG, Chaney JD, Mulla MS (1992) Identification of oviposition attractants for Culex quinquefasciatus from fermented Bermuda grass infusions. J Am Mos Contr Assoc 8:11–17Google Scholar
  16. Mordue AJ, Blackwell A, Hansson BS, Wadhams LJ, Pickett JA (1992) Behavioural and electrophysiological evaluation of oviposition attractants for Culex quinquefasciatus Say (Diptera: Culicidae). Experientia 48:1109–1111CrossRefGoogle Scholar
  17. Nathan SS, Kalaivani K, Sehoon K (2006a) Effects of Dysoxylum malabaricum Bedd. (Meliaceae) extract on the malaria vector Anopheles stephensi Liston (Diptera: Culicidae). Biores Technol 97:2077–2083CrossRefGoogle Scholar
  18. Nathan SS, Savitha G, George DK, Narmadha A, Suganya L, Chung PG (2006b) Efficacy of Melia azedarach L. extract on the malaria vector Anopheles stephensi Liston (Diptera: Culicidae). Biores Technol 97:1316–1323CrossRefGoogle Scholar
  19. Pandey DK, Chandra H, Tripathi NN, Dixit SN (1984) Mycotoxicity in leaves of some higher plants with special reference to that of Ageratum houstonianum Mill. Mykosen 26:565–573CrossRefGoogle Scholar
  20. Rajkumar S, Jebanesan A (2005) Oviposition deterrent and skin repellent activities of Solanum trilobatum leaf extract against the malaria vector Anopheles stephensi. J Insect Sci 5(15):1–3Google Scholar
  21. Rajkumar S, Jebanesan A (2008) Bioactivity of flavonoid compounds from Poncirus trifoliata L. (Family: Rutaceae) against the dengue vector Aedes aegypti (Diptera: Culicidae). Parasitol Res 104:19–25PubMedCrossRefGoogle Scholar
  22. Ravindran J, Samuel T, Alex E, William J (2012) Adulticidal activity of Ageratum houstonianum Mill. (Asteraceae) leaf extracts against three vector mosquito species (Diptera: Culicidae). Asian Pac J Trop Dis 2(3):177–179CrossRefGoogle Scholar
  23. Sakthivadivel M, Daniel T (2008) Evaluation of certain insecticidal plants for the control of vector mosquitoes viz., Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti. Appl Entomol Zool 43(1):57–63CrossRefGoogle Scholar
  24. Samuel T, Balaraju K, Kyungseok P, Raja AK, Ravindran KJ, Alex E, William SJ (2011a) In vitro antimicrobial activity of Ageratum houstonianum Mill. (Asteraceae). Food Sci 35:2897–2900Google Scholar
  25. Samuel T, Ravindran J, Arivoli S (2011b) Screening of plant extracts for ovicidal activity against Culex quinquefasciatus Say (Diptera: Culicidae). Appl Bot 40:5456–5460Google Scholar
  26. Senthilkumar N, Varma P, Gurusubramanian G (2009) Larvicidal and adulticidal activities of some medicinal plants against the malaria vector, Anopheles stephensi (Liston). Parasitol Res 104:237–244PubMedCrossRefGoogle Scholar
  27. Sharma RN, Joshi V, Jadu G, Bhosale AS, Gupta AS (1981) Oviposition deterrence activity in some Lamiaceae plants against insect pests. Z Naturforsh Teil C 36:122–125Google Scholar
  28. Su T, Mulla MS (1999) Ovipositional bioassay responses of Culex tarsalis and Culex quinquefasciatus to neem products containing azadirachtin. Entomol Exp Appl 91:337–345CrossRefGoogle Scholar
  29. Takken W, Knols BGJ (1999) Odor mediated behaviour of afrotropical malaria mosquitoes. Annu Rev Entomol 44:131–157PubMedCrossRefGoogle Scholar
  30. Wiedenfeld H, Cetto AA (2001) Pyrrolizidine alkaloids from Ageratum houstonianum Mill. Phytochem 57:1269–1271CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Samuel Tennyson
    • 1
    Email author
  • K. John Ravindran
    • 2
  • Alex Eapen
    • 2
  • S. John William
    • 3
  1. 1.Department of ZoologyMadras Christian CollegeChennaiIndia
  2. 2.National Institute of Malaria Research (ICMR) Field UnitChennaiIndia
  3. 3.Department of Advanced Zoology and Biotechnology, School of Entomology and Centre for Natural Resources Management (SECNARM)Loyola CollegeChennaiIndia

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