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Parasitology Research

, Volume 111, Issue 4, pp 1437–1445 | Cite as

New insight into the morphology of Eurytrema coelomaticum (Trematoda, Dicrocoeliidae) cercariae by light, scanning, and transmission electron microscopies

  • Jairo Pinheiro
  • Daniele Oliveira Franco-Acuña
  • Aleksandra Oliveira-Menezes
  • Solange Viana Paschoal Blanco Brandolini
  • Renato Augusto DaMatta
  • Wanderley de Souza
Original Paper

Abstract

Eurytrema coelomaticum is a digenetic trematode that parasitizes the pancreatic ducts of ruminants. In the present study, the morphology of the cercariae was analyzed using light, scanning, and transmission electron microscopies. The size of the larvae was larger than that reported in the literature. An oral sucker with many papillae and an oral aperture in its center and a ventral sucker with few discrete papillae on the edge were observed. No stylet at the anterior end of the larval body and spines on the tegument of the end of the tail were observed. The cercariae had lateral penetration glands and (central) pre-acetabular glands, from which ducts lead to the anterior region and open into a small anterior pocket. The flame cells were located laterally and communicated with fine branches, converging to two lateral excretory collecting ducts that opened into an excretory bladder, centrally located at the posterior end of the body. The tegument presented an external layer loosely attached to the larval body, below which an amorphous syncytial outer layer with many mitochondrial profiles was observed. This region exhibited many secretions and released secretory granules, indicating intense secretory activity. The circular and longitudinal muscle layers were arranged in sequence below the outer layer. The outer layer was connected by cytoplasmic bridges crossing the muscular layers to the internal region of the tegument, where the cell body with nucleus was located. In the cercarial body, it was possible to observe a typical flame cell with the barrel region, where cilia and the internal and external ribbed regions were found. The somatic cells seem to have an active metabolism, with a well-developed endoplasmic reticulum, secretory granules, and evident nuclei. The results are discussed in the light of the biology and taxonomy of this species.

Keywords

Ventral Sucker Oral Sucker Snail Host Longitudinal Muscle Layer Digenetic Trematode 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Beatriz Ferreira Ribeiro, Giovana Alves de Moraes, and Márcia Adriana da Silva for technical support. Financial support was by Fundação de Coordenação de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), and Financiadora de Estudos e Projetos (FINEP). JP is a FAPERJ post-doctoral and RAD and WS are CNPq fellows.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Jairo Pinheiro
    • 1
  • Daniele Oliveira Franco-Acuña
    • 2
  • Aleksandra Oliveira-Menezes
    • 3
  • Solange Viana Paschoal Blanco Brandolini
    • 4
  • Renato Augusto DaMatta
    • 5
  • Wanderley de Souza
    • 6
  1. 1.Área de Biofísica, Departamento de Ciências FisiológicasInstituto de Biologia, UFRRJSeropédicaBrazil
  2. 2.Curso de Pós-Graduação em Ciências Veterinárias, Departamento de Parasitologia AnimalInstituto de Veterinária, UFRRJSeropédicaBrazil
  3. 3.Grupo de Sistemática e Biologia Evolutiva (GSE), Pólo BarretoUniversidade Federal do Rio de Janeiro - Campus Macaé - Avenida São José do BarretoMacaéBrazil
  4. 4.Área de Biologia, Departamento de Biologia AnimalInstituto de Biologia, UFRRJSeropédicaBrazil
  5. 5.Laboratorio de Biologia Celular e TecidualCentro de Biociências e Biotecnologia, UENFCampos dos GoytacazesBrazil
  6. 6.Laboratório de Ultraestrutura Celular Hertha Meyer, Programa de Biologia Celular e Parasitologia, Instituto de Biofísica Carlos Chagas FilhoCentro de Ciências da Saúde, UFRJSeropédicaBrazil

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