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Parasitology Research

, Volume 111, Issue 2, pp 859–864 | Cite as

Ultrastructural study of the spermatozoon of the digenean Enodiotrema reductum Looss, 1901 (Platyhelminthes, Plagiorchioidea, Plagiorchiidae), parasite of the green turtle Chelonia mydas (Linnaeus, 1758) in Senegal

  • Papa Ibnou Ndiaye
  • Yann QuilichiniEmail author
  • Aminata Sène
  • Vasyl V. Tkach
  • Cheikh Tidiane Bâ
  • Bernard Marchand
Original Paper

Abstract

This study describes the ultrastructural organisation of the spermatozoon of a digenean Enodiotrema reductum (Pligiorchiida: Plagiorchiidae) from the green turtle Chelonia mydas (Linnaeus, 1758). This is the first report of E. reductum from Senegal. The mature spermatozoon of E. reductum is filiform and exhibits two axonemes of the 9 + “1” pattern of the Trepaxonemata, a nucleus, parallel cortical microtubules, an extramembranar ornamentation associated with spine-like bodies and granules of glycogen, among other ultrastructural features. The spermatozoon of E. reductum is distinguished by the presence of a moniliform mitochondrion composed of a bulge associated with a long cord and of a central cytoplasmic expansion. This work represents the first utrastructural study of any representative of the large family Plagiorchiidae. Our results are compared with previously published data on spermatozoa of other digenean taxa.

Keywords

Green Turtle Sodium Cacodylate Buffer Mature Spermatozoon Cortical Microtubule Posterior Extremity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank the fishermen of Ouakam (Dakar, Senegal) for providing the specimen used in this study.

References

  1. Agostini S, Miquel J, Ndiaye PI, Marchand B (2005) Dicrocoelium hospes Looss, 1907 (Digenea, Dicrocoeliidae): spermiogenesis, mature spermatozoon and ultrastructural comparative study. Parasitol Res 96:38–48PubMedCrossRefGoogle Scholar
  2. Bâ CT, Marchand B (1995) Spermiogenesis, spermatozoa and phyletic affinities in the Cestoda. In: Jamieson BGM, Ausió J, Justine J-L (Eds) Advances in spermatozoal phylogeny and taxonomy. Mém Mus Nat Hist Nat 166:87–95Google Scholar
  3. Bâ CT, Ndiaye PI, Dione A, Quilichini Y, Marchand B (2011) Ultrastructure of the spermatozoon of Holorchis micracanthum (Digenea: Lepocreadiidae), an intestinal parasite of Plectorhincus mediterraneus (Pisces, Teleostei) in Senegal. Parasitol Res 109:1099–1106PubMedCrossRefGoogle Scholar
  4. Bakhoum AJS, Bâ CT, Shimalov VV, Torres J, Miquel J (2011a) Spermatological characters of the digenean Rubenstrema exasperatum (Rudolphi, 1819) (Plagirochioidea, Omphalometridae). Parasitol Res 108:1283–1293PubMedCrossRefGoogle Scholar
  5. Bakhoum AJS, Torres J, Shimalov VV, Bâ CT, Miquel J (2011b) Spermiogenesis and spermatozoon ultrastructure of Diplodiscus subclavatus (Pallas, 1760) (Paramphistomoidea, Diplodiscidae), an intestinal fluke of the pool frog Rana lessonae. Parasitol Int 60:64–74PubMedCrossRefGoogle Scholar
  6. Burton PR (1972) Fine structure of the reproductive system of a frog lung-fluke. III. The spermatozoon and its differentiation. J Parasitol 58:68–83PubMedCrossRefGoogle Scholar
  7. Castilho F, Barandela T (1990) Ultrastructural study on the spermiogenesis and spermatozoon of the metacercariae of Microphallus primas (Digenea), a parasite of Carcinus maenas. Mol Reprod Dev 25:140–146PubMedCrossRefGoogle Scholar
  8. Ehlers U (1984) Phylogenetisches System der Plathelminthes. Verh Natwiss Ver Ham 27:291–294Google Scholar
  9. Foata J, Quilichini Y, Greani S, Marchand B (2012) Sperm ultrastructure of the digenean Aphallus tubarium (Rudolphi, 1819) Poche, 1926 (Platyhelminthes, Cryptogonimidae) intestinal parasite of Dentex dentex (Pisces, Teleostei). Tissue Cell 44:15–21PubMedCrossRefGoogle Scholar
  10. Iomini C, Justine J-L (1997) Spermiogenesis and spermatozoon of Echinostoma caproni (Platyhelminthes, Digenea): transmission and scanning electron microscopy, and tubulin immunocytochemistry. Tissue Cell 29:107–118PubMedCrossRefGoogle Scholar
  11. Jamieson BGM, Daddow LM (1982) The ultrastructure of the spermatozoon of Neochasmus sp. (Cryptogonimidae, Digenea, Trematoda) and its phylogenetic significance. Int J Parasitol 12:547–559CrossRefGoogle Scholar
  12. Justine J-L (1991) Spermatozoa as a tool for taxonomy of species and supraspecific taxa in the Platyhelminthes. In: Bacceti B (ed) Comparative spermatology 20 years after. Raven, New York, pp 981–984Google Scholar
  13. Justine J-L (1998) Spermatozoa as phylogenetic characters for the Eucestoda. J Parasitol 84:385–408PubMedCrossRefGoogle Scholar
  14. Justine J-L, Mattei X (1982) Réinvestigation de l’ultrastructure du spermatozoïde d’Haematoloechus (Trematoda: Haematoloechidae). J Ultra Res 81:322–332CrossRefGoogle Scholar
  15. Levron C, Ternengo S, Marchand B (2003) Ultrastructure of spermiogenesis and the spermatozoon of Helicometra fasciata (Digenea, Opecoelidae), a parasite of Labrus merula (Pisces, Teleostei). Acta Parasitol 48:255–264Google Scholar
  16. Levron C, Ternengo S, Marchand B (2004) Spermiogenesis and sperm ultrastructure of Poracanthium furcatum (Digenea, Opecoelidae), a parasite of Mullus surmuletus (Pisces, Teleostei). Acta Parasitol 49:190–200Google Scholar
  17. Levron C, Miquel J, Oros M, Scholz T (2010) Spermatozoa of tapeworms (Platyhelminthes, Eucestoda): advances in ultrastructural and phylogenetic studies. Biol Rev 85:523–543PubMedGoogle Scholar
  18. Miquel J, Nourrisson C, Marchand B (2000) Ultrastructure of spermiogenesis and the spermatozoon of Opecoeloides furcatus (Trematoda, Digenea, Opecoelidae), a parasite of Mullus barbatus (Pisces, Teleostei). Parasitol Res 86:301–310PubMedCrossRefGoogle Scholar
  19. Ndiaye PI, Miquel J, Bâ CT, Feliu C, Marchand B (2002) Spermiogenesis and sperm ultrastructure of Scaphiostomum palaearcticum Mas-Coma, Esteban et Valero, 1986 (Trematoda, Digenea, Brachylaimidae). Acta Parasitol 47:259–271Google Scholar
  20. Ndiaye PI, Miquel J, Feliu C, Marchand B (2003a) Ultrastructure of spermiogenesis and spermatozoa of Notocotylus neyrai González Castro, 1945 (Digenea, Notocotylidae), intestinal parasite of Microtus agrestis (Rodentia: Arvicolidae) in Spain. Invertebr Reprod Dev 43:105–115CrossRefGoogle Scholar
  21. Ndiaye PI, Miquel J, Fons R, Marchand B (2003b) Spermiogenesis and sperm ultrastructure of the liver fluke Fasciola hepatica L., 1758 (Digenea, Fasciolidae): scanning and transmission electron microscopy, and tubulin immunocytochemistry. Acta Parasitol 48:182–194Google Scholar
  22. Ndiaye PI, Miquel J, Bâ CT, Marchand B (2004) Spermiogenesis and ultrastructure of the spermatozoon of the liver fluke Fasciola gigantica Cobbold, 1856 (Digenea, Fasciolidae), a parasite of cattle in Senegal. J Parasitol 90:30–40PubMedCrossRefGoogle Scholar
  23. Ndiaye PI, Quilichini Y, Sène A, Bâ CT, Marchand B (2011) Ultrastructure of the spermatozoon of the digenean Cricocephalus albus (Kuhl & van Hasselt, 1822) Looss, 1899 (Platyhelminthes, Pronocephaloidea, Pronocephalidae), parasite of “the hawksbill sea turtle” Eretmochelys imbricata (Linnaeus, 1766) in Senegal. Zool Anz 250:215–222Google Scholar
  24. Olson PD, Cribb TH, Tkach VV, Bray RA, Littlewood DTJ (2003) Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). Int J Parasitol 33:733–755PubMedCrossRefGoogle Scholar
  25. Quilichini Y, Foata J, Marchand B (2007a) Ultrastructural study of the spermatozoon of Pronoprymna ventricosa (Digenea, Baccigerinae), parasite of the twaite shad Alosa fallax Lacepede (Pisces, Teleostei). Parasitol Res 101:1125–1130PubMedCrossRefGoogle Scholar
  26. Quilichini Y, Foata J, Orsini A, Marchand B (2007b) Ultrastructural study of spermiogenesis and the spermatozoon of Crepidostomum metoecus (Digenea: Allocreadiidae), a parasite of Salmo trutta (Pisces: Teleostei). J Parasitol 93:458–468PubMedCrossRefGoogle Scholar
  27. Quilichini Y, Foata J, Orsini A, Marchand B (2007c) Spermiogenesis and spermatozoon ultrastructure of Nicolla wisniewskii (Digenea: Opecoelidae), an intestinal parasite of brown trout Salmo trutta (Pisces: Teleostei). J Parasitol 93:469–478PubMedCrossRefGoogle Scholar
  28. Quilichini Y, Foata J, Justine J-L, Bray RA, Marchand B (2009) Sperm ultrastructure of the digenean Siphoderina elongata (Platyhelminthes, Cryptogonimidae) intestinal parasite of Nemipterus furcosus (Pisces, Teleostei). Parasitol Res 105:87–95PubMedCrossRefGoogle Scholar
  29. Quilichini Y, Foata J, Justine J-L, Bray RA, Marchand B (2010a) Ultrastructure study of the spermatozoon of Heterolebes maculosus (Digenea, Opistholebetidae), a parasite of the porcupinefish Diodon hystrix (Pisces, Teleostei). Parasitol Int 59:427–434PubMedCrossRefGoogle Scholar
  30. Quilichini Y, Foata J, Justine J-L, Bray RA, Marchand B (2010b) Spermatozoon ultrastructure of Aponurus laguncula (Digenea: Lecithasteridae), a parasite of Aluterus monoceros (Pisces, Teleostei). Parasitol Int 59:22–28PubMedCrossRefGoogle Scholar
  31. Quilichini Y, Foata J, Justine J-L, Bray RA, Marchand B (2011a) Sperm ultrastructure of Helicometra epinepheli (Platyhelminthes, Digenea, Opecoelidae), a parasite of Epinephelus fasciatus (Pisces, Teleostei). Histol Histopathol 26:1019–1028PubMedGoogle Scholar
  32. Quilichini Y, Foata J, Justine J-L, Bray RA, Marchand B (2011b) Spermatozoon ultrastructure of Gyliauchen sp. (Digenea: Gyliauchenidae), an intestinal parasite of Siganus fuscescens (Pisces: Teleostei). Biol Bull-US 221:197–205Google Scholar
  33. Seck MT, Marchand B, Bâ CT (2007) Ultrastructure of spermiogenesis and the spermatozoon of Paramphistomum microbothrium (Fischoeder 1901; Digenea, Paramphistomidae), a parasite of Bos taurus in Senegal. Parasitol Res 101:259–268PubMedCrossRefGoogle Scholar
  34. Seck MT, Marchand B, Bâ CT (2008a) Spermiogenesis and sperm ultrastructure of Carmyerius endopapillatus (Digenea, Gastrothylacidae), a parasite of Bos taurus in Senegal. Acta Parasitol 53:9–18CrossRefGoogle Scholar
  35. Seck MT, Marchand B, Bâ CT (2008b) Spermiogenesis and sperm ultrastructure of Cotylophoron cotylophorum (Trematoda, Digenea, Paramphistomidae), a parasite of Bos taurus in Senegal. Parasitol Res 103:157–166PubMedCrossRefGoogle Scholar
  36. Stitt AW, Fairweather I (1990) Spermatogenesis and the fine structure of the mature spermatozoon of the liver fluke, Fasciola hepatica (Trematoda: Digenea). Parasitology 101:395–407PubMedCrossRefGoogle Scholar
  37. Tang J, Wang W, Wang G (1998) Studies on ultrastructure of spermatogenesis and sperm in Pseudorhipidocotyle elpichthys. Acta Hydrobiol Sin 22(Suppl):168–173Google Scholar
  38. Thiéry JP (1967) Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J Microsc Paris 6:987–1018Google Scholar
  39. Tkach VV (2008) Family Plagiorchiidae Lühe, 1901. In: Bray RA, Gibson DI, Jones A (eds) Keys to the Trematoda. CABI, Wallingford, pp 295–325Google Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Papa Ibnou Ndiaye
    • 1
  • Yann Quilichini
    • 2
    Email author
  • Aminata Sène
    • 1
  • Vasyl V. Tkach
    • 3
  • Cheikh Tidiane Bâ
    • 1
  • Bernard Marchand
    • 2
  1. 1.Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and TechniquesCheikh Anta Diop University of DakarDakarSenegal
  2. 2.CNRS UMR 6134, Laboratory “Parasites and Mediterranean Ecosystems”University of CorsicaCorteFrance
  3. 3.Department of BiologyUniversity of North DakotaGrand ForksUSA

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