Therapeutic effect of alpha lipoic acid combined with praziquantel on liver fibrosis induced by Schistosoma mansoni challenged mice
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Schistosomiasis is an endemic disease in 74 countries causing more than 250,000 deaths every year. Accordingly, the development of an effective drug for eradication of schistosomiasis is an open research field. The current chemotherapy for control is praziquantel (PZQ). However, PZQ does not improve liver fibrosis. Therefore, the aim of this study is to evaluate the combined effect of alpha lipoic acid (ALA) with PZQ on the liver fibrosis induced by Schistosoma mansoni challenged mice. Evaluation was based on the worm burden count, ova load, granuloma size, and histopathology of the liver. Reduced glutathione (GSH) was measured in the tissue as a biomarker for impaired antioxidant function. Malondialdehyde (MDA) was also measured in the tissue as a biomarker for oxidative stress. The serum level of matrix metalloproteinase 1 was measured as a biomarker for fibrotic status of the liver. Liver function enzymes such as ALT, AST, and GGT were also measured. Four groups of ten mice each were used in this study. The first group was infected with 50 ± 10 S. mansoni cercariae. The second group was also infected and was treated with PZQ 9 weeks post-infection (PI). The third group was treated with PZQ and ALA 9 weeks PI. The fourth group was used a healthy control. The present study revealed remarkable improvement in all parameters measured (parasitological and biochemical) as well as significant improvement of hepatic pathology in the third group which was treated with PZQ and ALA. The treatment of mice with PZQ and ALA results in reduction in the worm burden, egg count, and granuloma size. Furthermore, this combined treatment increased the tissue level of the antioxidant (GSH) and decreased the tissue level of MDA in this group.
KeywordsLiver Fibrosis Schistosomiasis Praziquantel Infected Group Worm Burden
The authors would like to thank Prof. Nabil S. Gabr, Head of the Department of Parasitology, Faculty of Medicine, Minia University, Egypt, for his continuous advice in technical procedures. Thanks are extended to Prof. Nawras M. Mowafy, Professor of Parasitology, Faculty of Medicine, Minia University for her guiding us through the technical procedures. The authors would also like to thank Prof. Refaat M. A. Khalifa, Professor of Parasitology, Faculty of Medicine, Assuit University for his continuous advice.
- Cheever AW (1968) Condition affecting the accuracy of potassium hydroxide digestion technique for counting Schistosoma mansoni eggs in tissue. Bull World Health 39:328–31Google Scholar
- Seif el-Din SH, Ebeid FA, Badawy AA, Ezzat AR (2006) Protective effects of β-carotene, N-acetyl-cysteine with and without praziquantel treatment in Schistosoma mansoni-infected mice. Egyptian J Schistosomiasis Infect Endemic Dis 28:67–90Google Scholar
- El-Shenawy NS, Soliman MFM (2003) Evaluation of the protective effect of two antioxidative agents in mice experimentally induced with Schistosoma mansoni: biochemical and parasitological aspects. J Egypt Ger Zool 40(A):201–216Google Scholar
- Hammad H, Sherif S, Ebeid F (2000) Effect of praziquantel on some biochemical aspects in experimental schistosomiasis. Recent Res Dev Biotechnol Bioeng 3:107–118Google Scholar
- Harvie M, Jordan TW, La Flamme AC (2007) Differential liver protein expression during schistosomiasis infection and immunity. Infect Immun 75: 736-744Google Scholar
- Pellegrino J, Oliveria CA, Faria J, Cunha A (1962) New approach to the screening of the drugs in experimental Schistosoma mansoni in mice. Am J Trop Med Hyg 1:201–15Google Scholar
- Xiong LJ, Zhu JF, Luo DD, Zen LL, Cai SQ (2003) Effects of pentoxifylline on the hepatic content of TGF-β1 and collagen in schistosomiasis japonica mice with liver fibrosis. World J Gastroent 9:152–154Google Scholar
- Zhang BB, Cai WM, Weng HL, Hu ZR, Lu J, Zheng M, Liu RH (2003) Diagnostic value of platelet derived growth factor-BB, transforming growth factor-beta matrix metalloproteinase-1 and tissue inhibitor of matrix metalloproteinase-1 in serum and peripheral blood mononuclear cells for hepatic fibrosis. World J Gastroenterol 9:2490–6PubMedGoogle Scholar
- Zou WL, Yang Z, Zang YJ, Li DJ, Liang ZP, Shen ZY (2007) Inhibitory effects of prostaglandin E1 on activation of hepatic stellate cells in rabbits with schistosomiasis. Hepatobiliary Pancreatol Distrib Int 6:176–181Google Scholar