Parasitology Research

, Volume 111, Issue 1, pp 383–392 | Cite as

Molecular characterization of Acanthamoeba isolated in water treatment plants and comparison with clinical isolates

  • A. Magnet
  • A. L. Galván
  • S. Fenoy
  • F. Izquierdo
  • C. Rueda
  • C. Fernandez Vadillo
  • J. Pérez-Irezábal
  • K. Bandyopadhyay
  • G. S. Visvesvara
  • A. J. da Silva
  • C. del AguilaEmail author
Original Paper


A total of 116 samples (44 clinical specimens and 72 environmental samples) have been analyzed for the presence of Acanthamoeba. The environmental samples (ESs) were collected from four drinking water treatment plants (DWTP, n = 32), seven wastewater treatment plants (n = 28), and six locations of influence (n = 12) on four river basins from the central area of Spain (winter–spring 2008). Water samples were concentrated by using the IDEXX Filta-Max® system. Acanthamoeba was identified in 65 of the 72 ESs by culture isolation (90.3%) and 63 by real-time PCR (87.5%), resulting in all sampling points (100%) positive for Acanthamoeba when considering both techniques and all the time period analyzed. Nine of the 44 clinical specimens were positive for Acanthamoeba. Seventeen Acanthamoeba strains (eight from four DWTP and nine from clinical samples) were also established in axenic-PYG medium. Twenty-four of the ESs and the 17 Acanthamoeba sp. strains were genotyped as T4/1, T4/8, and T4/9. The eight strains isolated from the DWTP samples were inoculated in nude mouse to ascertain their potential pathogenicity in this model. Animals that were inoculated died or showed central nervous system symptoms 9 days post-inoculation. Examination of immunofluorescence-stained brain and lung tissue sections showed multiple organisms invading both tissues, and re-isolation of throphozoites was successful in these tissues of all infected animals. For the first time, potentially pathogenic Acanthamoeba T4 has been detected in 100% of different types of water samples including tap water and sewage effluents in the central area of Spain suggesting a potential health threat for humans especially for the contact lens wearers.


Central Nervous System Infection Finished Water Acanthamoeba Keratitis Drinking Water Treatment Plant Acanthamoeba Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are indebted to Sergio Llorens for his valuable technical assistance, to Brian Crilly for helpful revision of the manuscript, to Santiago Angulo for their participation on the discussion of the results, and to Francisco J. Carrillo for help in artwork. We would like to thank Canal Isabel II, especially Carmen Gordo and Joseba Iñaki Urrutia for their help in designing and carrying out the sampling collection and also for their useful discussion of the results. This work was funded by grant PI061593 from Instituto de Salud Carlos III (FISS) and by grant PC 04/07 of the Fundación Universitaria San Pablo CEU. Ana Luz Galvan was supported in Spain by an overseas fellowship from Colciencias [Antioquía University (Colombia)]. Angela Magnet was supported by an undergraduate and a predoctoral fellowship from Fundación Universitaria San Pablo CEU.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • A. Magnet
    • 1
  • A. L. Galván
    • 1
    • 2
  • S. Fenoy
    • 1
  • F. Izquierdo
    • 1
  • C. Rueda
    • 1
  • C. Fernandez Vadillo
    • 1
  • J. Pérez-Irezábal
    • 3
  • K. Bandyopadhyay
    • 4
  • G. S. Visvesvara
    • 5
  • A. J. da Silva
    • 4
  • C. del Aguila
    • 1
    • 6
    Email author
  1. 1.Parasitology LaboratorySan Pablo CEU University, Urbanización MontepríncipeMadridSpain
  2. 2.Grupo de Parasitología. Escuela de MicrobiologíaUniversidad de AntioquiaMedellínColombia
  3. 3.Hospital CrucesVizcayaSpain
  4. 4.Center for Global Health, Division of Parasitic Diseases and MalariaCenters for Disease Control and PreventionAtlantaUSA
  5. 5.National Center for Emerging and Zoonotic Infectious Diseases, Division of Foodborne, Waterborne and Environmental DiseasesCenters for Disease Control and PreventionGAUSA
  6. 6.Facultad de FarmaciaUrbanización MontepríncipeMadridSpain

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