Findings related to IL-8 and IL-10 gene polymorphisms in a Mexican patient population with irritable bowel syndrome infected with Blastocystis
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The intestinal protozoan parasite Blastocystis is one of the most common parasites worldwide in humans and, although its ability to cause human disease has been questioned, some reports have demonstrated that this microorganism is associated to the development of irritable bowel syndrome (IBS) and to a proinflammatory response, in which the expression of some cytokines is unregulated. Since inflammatory cytokine gene polymorphisms might have a role in the pathophysiology of IBS, we assessed the role of single nucleotide polymorphisms (SNPs) for interleukin (IL)-8 and IL-10, in previously collected DNA samples from IBS patients and controls, with or without Blastocystis infection. IL-8+396(G) and IL-10-1082 (A) alleles (p = 0.0437 and p = 0.0267, respectively), as well as their homozygous (p < 0.0001 and p = 0.0039, respectively) and IL-8+781(CT) (p = 0.0248) genotypes were significantly overrepresented in patients with IBS in comparison with controls. IL-8+396(GG) genotype was relevant because it was associated to IBS (p < 0.0001), to Blastocystis (p = 0.0025), and to IBS–Blastocystis (p = 0.0272). In the latter binomial association, this genotype presented a high contribution (etiological fraction = 0.452) and a risk >fourfold to develop IBS. IL-8+781 (T) and IL-10-592 (C) alleles were also associated to Blastocystis and to IBS–Blastocystis, respectively (p = 0.0448 and p = 0.0166). Our results suggest that some IL-8 and IL-10 SNPs could change individual susceptibility increasing the relative risk in the development of IBS in Blastocystis carriers.
KeywordsIrritable Bowel Syndrome Diverticulitis Irritable Bowel Syndrome Patient Proinflammatory Response Homozygous Variant
The authors gratefully acknowledge Maria Elena Rodriguez-Campa and Alberto Gonzalez-Angulo for their clinical assistance. Rocio Jimenez-Lucio and David Sierra performed DNA extractions. This work was partially supported by CONACYT grant 69589.
- Barkhordari E, Rezaei N, Ansaripour B, Larki P, Alighardashi M, Ahmadi-Ashtiani HR, Mahmoudi M, Keramati MR, Habibollahi P, Bashashati M, Ebrahimi-Daryani N, Amirzargar AA (2010a) Proinflamatory cytokine gene polymorphisms in irritable bowel syndrome. J Clin Immunol 30:74–79PubMedCrossRefGoogle Scholar
- Barkhordari E, Rezaei N, Mahmoudi M, Larki P, Ahmadi-Ashtiani HR, Ansaripour B, Alighardashi M, Bashashati M, Amirzargar AA, Ebrahimi-Daryani N (2010b) T-helper 1, T-helper 2, and T-regulatory cytokines gene polymorphisms in irritable bowel syndrome. Inflammation 33:281–286PubMedCrossRefGoogle Scholar
- Jimenez-Gonzalez DE, Martinez-Flores WA, Reyes-Gordillo J, Ramirez-Miranda ME, Arroyo-Escalante S, Romero-Valdovinos M, Stark D, Souza-Saldivar V, Martinez-Hernandez F, Flisser A, Olivo-Diaz A, Maravilla P (2011) Blastocystis infection is associated with irritable bowel syndrome in a Mexican patient population. Parasitol Res. doi: 10.1007/s00436-011-2626-7
- Sambrook J, Fitsch EF, Maniatis T (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor, New YorkGoogle Scholar
- Yakoob J, Jafri W, Jafri N, Khan R, Islam M, Beg MA, Zaman V (2004) Irritable bowel syndrome: in search of an etiology: role of Blastocystis hominis. AmJTrop Med Hyg 70:383–385Google Scholar