Parasitology Research

, Volume 110, Issue 5, pp 1795–1800 | Cite as

In vitro and in vivo antiparasitic activity of Azadirachtin against Argulus spp. in Carassius auratus (Linn. 1758)

  • Saurav KumarEmail author
  • R. P. Raman
  • Kundan Kumar
  • P. K. Pandey
  • Neeraj Kumar
  • Snatashree Mohanty
  • Abhay Kumar
Original Paper


Argulus is one of the most common and predominant ectoparasites which cause serious parasitic disease and is a potent carrier of viruses and bacteria in the ornamental fish industry. In recent years, organic (herbs)-based medicines are widely used to cure the disease, and neem (Sarbaroganibarini) medicine is very popular and effective throughout the world. The present study was conducted to find the effects of Azadirachtin against Argulus spp. on Carassius auratus under in vitro and in vivo conditions. The 96-h median lethal concentration (LC50) for Azadirachtin EC 25% against Carassius auratus was found to be 82.115 mg L−1. The antiparasitic activity test under in vitro and in vivo was evaluated at 1 (T1), 5 (T2), 10 (T3), 15 (T4) and 20 mg L−1 (T5) to treat Argulus for 3 h and 72 h, respectively. In vitro effect of Azadirachtin solution led to 100% mortality of Argulus at 20 and 15 mg L−1 for 2.5 and 3 h, respectively. Whereas, under in vivo test, the 100% antiparasitic efficacy of Azadirachtin solution was found at 15 and 20 mg L−1 for 72 and 48 h, respectively. The EC50 for 48 h was 20 mg L−1, and thus, therapeutic index is 4.10. The results provided evidence that Azadirachtin can be used as a potential agent for controlling Argulus.


Cypermethrin Azadirachtin Dichlorvos Head Louse Median Lethal Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are thankful to W. S. Lakra, the Director and Vice Chancellor of the Central Institute of Fisheries Education, Mumbai, India, for providing all the facilities required for the present work.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Saurav Kumar
    • 1
    Email author
  • R. P. Raman
    • 1
  • Kundan Kumar
    • 1
  • P. K. Pandey
    • 1
  • Neeraj Kumar
    • 1
  • Snatashree Mohanty
    • 1
  • Abhay Kumar
    • 1
  1. 1.Central Institute of Fisheries EducationMumbaiIndia

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