Parasitology Research

, Volume 110, Issue 5, pp 1779–1783 | Cite as

In vitro antileishmanial and antitrypanosomal activities of five medicinal plants from Burkina Faso

  • W. R. SawadogoEmail author
  • G. Le Douaron
  • A. Maciuk
  • C. Bories
  • P. M. Loiseau
  • B. Figadère
  • I. P. Guissou
  • O. G. Nacoulma
Original Paper


After ethnobotanical surveys in central and western regions of Burkina Faso, five plants namely Lantana ukambensis (Verbenaceae), Xeoderris sthulmannii (Fabaceae), Parinari curatellifollia (Chrysobalanaceae), Ozoroa insignis (Anacardiaceae), and Ficus platyphylla (Moraceae) were selected for their traditional use in the treatment of parasitic diseases and cancer. Our previous studies have focused on the phytochemical, genotoxicity, antioxidant, and antiproliferative activities of these plants. In this study, the methanol extract of each plant was tested to reveal probable antileishmanial and antitrypanosomal activities. Colorimetric and spectrophotometric methods were used for the detection of antileishmanial and antitrypanosomal activities. Leishmania donovani (LV9 WT) and Trypanosoma brucei brucei GVR 35 were used to test the antileishmanial and antitrypanosomal activities, respectively. All extracts of tested plants showed a significant antitrypanosomal activity with minimum lethal concentrations between 1.5 and 25 μg/ml, the L. ukambensis extract being the most active. In the antileishmanial test, only the extract from L. ukambensis showed significant activity with an inhibitory concentration (IC50) of 6.9 μg/ml. The results of this study contribute to the promotion of traditional medicine products and are preliminary for the isolation of new natural molecules for the treatment of leishmaniasis and trypanosomiasis.


Visceral Leishmaniasis Leishmaniasis Pentamidine Cutaneous Leishmaniasis African Human Trypanosomiasis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are grateful to the Agence Universitaire de la Francophonie (AUF) for providing financial support (postdoctoral grant).


  1. Aderbauer B, Clausen PH, Kershaw O, Melzig MF (2008) In vitro and in vivo trypanocidal effect of lipophilic extracts of medicinal plants from Mali and Burkina Faso. J Ethnopharmacol 119(2):225–231. doi: 10.1016/j.jep.2008.06.024 PubMedCrossRefGoogle Scholar
  2. Bern C, Maguire J, Alvar J (2008) Complexities for assessing the disease burden attributable to leishmaniasis. PLoS Negl Trop Dis 2(10):313CrossRefGoogle Scholar
  3. Chollet C, Crousse B, Bories C, Bonnet-Delpon D, Loiseau PM (2008) In vitro antileishmanial activity of fluoro-artemisinin derivatives against Leishmania donovani. Biomed Pharmacother 62(7):462–465PubMedCrossRefGoogle Scholar
  4. Croft SL, Barret MP, Urbina JA (2005) Chemoterapy of trypanosomiases and leishmaniasis. Trends Parasitol 21:508–509PubMedCrossRefGoogle Scholar
  5. Farnsworth NR, Akelere O, Bingel A, Soejarto DD, Guo Z (1985) Medicinal plants in therapy. In: WHO (ed) WHO Bull 63:965–981Google Scholar
  6. Fernanda GB et al (2007) Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil. J Ethnopharmacol 111:396–402CrossRefGoogle Scholar
  7. Filho AADS et al (2009) In vitro antileishmanial, antiplasmodial and cytotoxic activities of phenolics and triterpenoids from Baccharis dracunculifolia D. C. (Asteraceae). Fitoterapia 80(8):478–482CrossRefGoogle Scholar
  8. Ghosh S et al (2011) Valeriana wallichii root extracts and fractions with activity against Leishmania spp. Parasitol Res 108(4):861–871PubMedCrossRefGoogle Scholar
  9. González-Coloma A, et al (2011) Antileishmanial, antitrypanosomal, and cytotoxic screening of ethnopharmacologically selected Peruvian plants. Parasitol Res in press doi: 10.1007/s00436-011-2638-3
  10. Grecco SS et al (2010) Isolation of an antileishmanial and antitrypanosomal flavanone from the leaves of Baccharis retusa DC. (Asteraceae). Parasitol Res 106(5):1245–1248PubMedCrossRefGoogle Scholar
  11. Kam T-S, Sim K-M, Koyano T, Komiyama K (1999) Leishmanicidal alkaloids from Kopsia griffithii. Phytochemistry 50(1):75–79CrossRefGoogle Scholar
  12. Kamanzi AK, Schmid C, Brun R, Kone MW, Traore D (2004) Antitrypanosomal and antiplasmodial activity of medicinal plants from Cote d’Ivoire. J Ethnopharmacol 90(2–3):221–227. doi: 10.1016/j.jep.2003.09.032 CrossRefGoogle Scholar
  13. Kayser O, Kiderlen AF (2001) In vitro leishmanicidal activity of naturally occurring chalcones. Phytother Res 15:148–152PubMedCrossRefGoogle Scholar
  14. Kolodziej H et al (2001) Proanthocyanidins and related compounds: antileishamanial activity and modulatory effects on nitric oxide and tumor necrosis factor-a-release in the murine macrophage like cell line RAE 264.7. Biol Pharm Bull 24:1016–1021PubMedCrossRefGoogle Scholar
  15. Kouassi MA, Ioset J-R, Ioset KN, Diallo D, Mauël J, Hostettmann K (2007) Antileishmanial activities associated with plants used in the Malian traditional medicine. J Ethnopharmacol 110:99–104CrossRefGoogle Scholar
  16. Lira R et al (1999) Evidence that the high incidence of treatment failures in Indian kala-azar is due to the emergence of Antimony-resistant strains of Leishmania donovani. J Infect Dis 180:564–567PubMedCrossRefGoogle Scholar
  17. Liu M, Wilairat P, Croft SL, Tan AL, Go M (2003) Structure-activity relationships of antileishmanial and antimalarial chalcones. Bioorg Med Chem 11:2729–2738PubMedCrossRefGoogle Scholar
  18. Mohammad I et al (2003) Antiparasitic alkaloids from Psychotria klugii. I Natl Products 66:962–967CrossRefGoogle Scholar
  19. Murray H, Berman J, Davies C, Saravia N (2005) Advances in leishmaniasis. Lancet 366:1561–1577PubMedCrossRefGoogle Scholar
  20. Ngurea RM et al (1997) Major acute phase response of haptoglobin and serum amyloid-P following experimental infection of mice with Trypanosoma brucei brucei. Parasitol Int 46:247–254CrossRefGoogle Scholar
  21. Nok AJ, Williams S, Onyenekwe PC (1996) Allium sativum-induced death of African trypanosomes. Parasitol Res 82(7):634–637PubMedCrossRefGoogle Scholar
  22. Prytzyk E et al (2003) Flavonoids and trypanocidal activity of Bulgarian propolis. J Ethnopharmacol 88(2–3):189–193PubMedCrossRefGoogle Scholar
  23. Ridoux O et al (2001) In vitro antileishmanial activity of three saponins isolate from Ivy, a-hederin, FI-hederin and hederacolchiside A in association with pentamidine and amphotricin B. Phytother Res 15:298–301PubMedCrossRefGoogle Scholar
  24. Saklani A, Kutty S (2008) Plant-derived compounds in clinical trials. Drug Discov Today 13:161–171PubMedCrossRefGoogle Scholar
  25. Santin MR, dos Santos AO, Nakamura CV, Dias Filho BP, Ferreira IC, Ueda-Nakamura T (2009) In vitro activity of the essential oil of Cymbopogon citratus and its major component (citral) on Leishmania amazonensis. Parasitol Res 105(6):1489–1496PubMedCrossRefGoogle Scholar
  26. Santos DO et al (2008) Leishmaniasis treatment—a challenge that remains: a review. Parasitol Res 103(1):1–10PubMedCrossRefGoogle Scholar
  27. Sawadogo WR et al (2011) Mutagenic effect, antioxidant and anticancer activities of six medicinal plants from Burkina Faso. Nat Prod Res. doi: 10.1080/14786419.2010.534737
  28. Shuaibu MN et al (2008) Trypanocidal activity of extracts and compounds from the stem bark of Anogeissus leiocarpus and Terminalia avicennoides. Parasitol Res 102(4):697–703. doi: 10.1007/s00436-007-0815-1 PubMedCrossRefGoogle Scholar
  29. Tan N et al (2002) Abietane diterpenoids and triterpenoic acids from Salvia cilicica and their antileishmanial activities. Phytochem Rev 61:881–884CrossRefGoogle Scholar
  30. WHO (1998) Control and surveillance of African trypanosomiasis. WHO Tech Rep Ser. vol 881. World Health Organisation, GenevaGoogle Scholar
  31. WHO (2003) Traditional medicine fact sheet, vol 134. WHO, GenevaGoogle Scholar
  32. Zagana P, Klepetsanis P, Ioannou PV, Loiseau PM, Antimisiaris SG (2007) Trypanocidal activity of arsonoliposomes: effect of vesicle lipid composition. Biomed Pharmacother 61(8):499–504PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • W. R. Sawadogo
    • 1
    • 2
    Email author
  • G. Le Douaron
    • 2
  • A. Maciuk
    • 2
  • C. Bories
    • 3
  • P. M. Loiseau
    • 3
  • B. Figadère
    • 2
  • I. P. Guissou
    • 1
    • 4
  • O. G. Nacoulma
    • 5
  1. 1.Institut de Recherche en Sciences de la Santé (IRSS/CNRST)Ouagadougou 03Burkina Faso
  2. 2.Laboratoire de chimie des substances naturelles, UMR-8076 BioCis, Labex LERMIT, Faculté de Pharmacie de Châtenay-MalabryUniv Paris-SudChâtenay-MalabryFrance
  3. 3.Chimiothérapie antiparasitaire/UMR-8076 BioCis, Labex LERMIT, Faculté de Pharmacie de Châtenay-MalabryUniv Paris-SudChâtenay-MalabryFrance
  4. 4.Département de Sciences Pharmaceutiques appliquées/Pharmacologie–toxicologieUFR/SDS/Université de OuagadougouOuagadougouBurkina Faso
  5. 5.Laboratoire de Biochimie et Chimie appliquéesUFR/SVT Université de OuagadougouOuagadougouBurkina Faso

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