Parasitology Research

, Volume 110, Issue 5, pp 1747–1754

In vitro efficacy of the essential oil of Piper cubeba L. (Piperaceae) against Schistosoma mansoni

  • Lizandra G. Magalhães
  • Julia M. de Souza
  • Kamila A. L. Wakabayashi
  • Rosangela da S. Laurentiz
  • Adriana H. C. Vinhólis
  • Karen C. S. Rezende
  • Guilherme V. Simaro
  • Jairo K. Bastos
  • Vanderlei Rodrigues
  • Viviane R. Esperandim
  • Daniele S. Ferreira
  • Antônio E. M. Crotti
  • Wilson R. Cunha
  • Márcio L. A. e Silva
Original Paper
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Abstract

In this paper, cercariae, schistosomula, and adult Schistosoma mansoni worms were incubated in vitro with the essential oil of Piper cubeba (PC-EO) at concentrations from 12.5 to 200 μg/mL, and the viability was evaluated using an inverted microscopy. The effects of PC-EO at 100 and 200 μg/mL on the stages of S. mansoni were similar to those of the positive control (PZQ at 12.5 μg/mL), with total absence of mobility after 120 h. However, at concentrations from 12.5 to 50 μg/mL, PC-EO caused a reduction in the viability of cercariae and schistosomula when compared with the negative control groups (RPMI 1640 or dechlorinated water) or (RPMI 1640 + 0.1% DMSO or dechlorinated water + 0.1% DMSO). On the other hand, adult S. mansoni worms remained normally active when incubated with PC-EO at concentrations of 12.5 and 25 μg/mL, and their viabilities were similar to those of the negative control groups. In addition, at concentrations ranging from 50 to 200 μg/mL, separation of all the coupled adult worms was observed after 24 h of incubation, which is related to the fact of the reduction in egg production at this concentration. The main chemical constituents of PC-EO were identified by gas chromatography-mass spectrometry as being sabinene (19.99%), eucalyptol (11.87%), 4-terpineol (6.36%), β-pinene (5.81%), camphor (5.61%), and δ-3-carene (5.34%). The cytotoxicity of the PC-EO was determined, and a significant cytotoxicity was only obtained in the concentration of 200 μg/mL after 24 h treatment. The results suggest that PC-EO possesses an effect against cercariae, schistosomula, and adult worms of the S. mansoni.

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Lizandra G. Magalhães
    • 1
  • Julia M. de Souza
    • 1
  • Kamila A. L. Wakabayashi
    • 1
  • Rosangela da S. Laurentiz
    • 3
  • Adriana H. C. Vinhólis
    • 1
  • Karen C. S. Rezende
    • 1
  • Guilherme V. Simaro
    • 1
  • Jairo K. Bastos
    • 4
  • Vanderlei Rodrigues
    • 2
  • Viviane R. Esperandim
    • 1
  • Daniele S. Ferreira
    • 1
  • Antônio E. M. Crotti
    • 1
  • Wilson R. Cunha
    • 1
  • Márcio L. A. e Silva
    • 1
  1. 1.Núcleo de Pesquisas em Ciências Exatas e TecnológicasUniversidade de FrancaFrancaBrazil
  2. 2.Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil
  3. 3.Departamento de Física e Química, Faculdade de Engenharia de Ilha SolteiraUniversidade Estadual PaulistaIlha SolteiraBrazil
  4. 4.Departamento de Ciências Farmacêuticas da Faculdade de Farmácia de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil

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