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Parasitology Research

, Volume 110, Issue 4, pp 1473–1479 | Cite as

Morphological and molecular evidence for a new species of the genus Raphidascaris (Nematoda: Anisakidae) from marine fishes from the South China Sea

  • Liang Li
  • Yuan-Yuan Liu
  • Bao-Chen Liu
  • Lu-Ping ZhangEmail author
Original Paper

Abstract

A new anisakid nematode, Raphidascaris (Ichthyascaris) longispicula sp. nov., is described from the intestine and stomach of the marine fish Uroconger lepturus (Richardson) (Anguilliformes: Congridae) from the South China Sea (Guangdong and Hainan Province, respectively). The new species differs from all congeners in the subgenus Ichthyascaris by the short ventricular appendix (0.36–0.49 mm long), the number and arrangement of caudal papillae (25–28 pairs of preanal, 1–2 pairs of paranal, and 6–8 pairs of postanal), and the very long spicules (1.13–1.32 mm long, representing 9.34–10.3% of body length). The sequences of the internal transcribed spacer of ribosomal DNA of R. (I.) longispicula sp. nov. are analyzed and compared with the closely related nematode sequences. Molecular analyses seem to support the validity of this new nematode species based on morphological observation.

Keywords

Internal Transcribe Spacer Morphological Observation Nematode Species Formaldehyde Solution Hainan Province 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors wish to thank Professor Ruiyong An (College of Life Sciences, Hebei Normal University) for identifying the fishes. This study was supported by the National Natural Science Foundation of China (NSFC-30970318, 31101615) and a grant (No. L2010B13) from the Hebei Normal University to Dr. Liang Li.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Liang Li
    • 1
  • Yuan-Yuan Liu
    • 1
  • Bao-Chen Liu
    • 2
  • Lu-Ping Zhang
    • 1
    Email author
  1. 1.College of Life ScienceHebei Normal UniversityShijiazhuangPeople’s Republic of China
  2. 2.Botou Vocational CollegeBotouPeople’s Republic of China

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