Parasitology Research

, Volume 110, Issue 4, pp 1473–1479 | Cite as

Morphological and molecular evidence for a new species of the genus Raphidascaris (Nematoda: Anisakidae) from marine fishes from the South China Sea

  • Liang Li
  • Yuan-Yuan Liu
  • Bao-Chen Liu
  • Lu-Ping ZhangEmail author
Original Paper


A new anisakid nematode, Raphidascaris (Ichthyascaris) longispicula sp. nov., is described from the intestine and stomach of the marine fish Uroconger lepturus (Richardson) (Anguilliformes: Congridae) from the South China Sea (Guangdong and Hainan Province, respectively). The new species differs from all congeners in the subgenus Ichthyascaris by the short ventricular appendix (0.36–0.49 mm long), the number and arrangement of caudal papillae (25–28 pairs of preanal, 1–2 pairs of paranal, and 6–8 pairs of postanal), and the very long spicules (1.13–1.32 mm long, representing 9.34–10.3% of body length). The sequences of the internal transcribed spacer of ribosomal DNA of R. (I.) longispicula sp. nov. are analyzed and compared with the closely related nematode sequences. Molecular analyses seem to support the validity of this new nematode species based on morphological observation.


Internal Transcribe Spacer Morphological Observation Nematode Species Formaldehyde Solution Hainan Province 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors wish to thank Professor Ruiyong An (College of Life Sciences, Hebei Normal University) for identifying the fishes. This study was supported by the National Natural Science Foundation of China (NSFC-30970318, 31101615) and a grant (No. L2010B13) from the Hebei Normal University to Dr. Liang Li.


  1. Bruce NL (1990) Hysterothylacium Ward and Magath, 1917, and Ichthyascaris Wu, 1949, ascaridoid nematodes from Australian demersal fishes. Mem Quee Mus 28:389–426Google Scholar
  2. Bruce NL, Adlard RD, Cannon LRG (1994) Synoptic checklist of ascaridoid parasites (Nematoda) from fish hosts. Invertebr Taxon 8:583–674CrossRefGoogle Scholar
  3. D’Amelio S, Mathiopoulos KD, Santos CP, Pugachev ON, Webb SC, Picanco M, Paggi L (2000) Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. Int J Parasitol 30:223–226PubMedCrossRefGoogle Scholar
  4. Du CX, Zhang LP, Shi MQ, Ming Z, Hu M, Gasser RB (2010) Elucidating the identity of Anisakis larvae from a broad range of marine fishes from the Yellow Sea, China, using a combined electrophoretic-sequencing approach. Electrophoresis 31:654–658PubMedCrossRefGoogle Scholar
  5. Fang WZ, Xu SS, Zhang SL, Wang YN, Chen XB, Luo DM (2010) Multiple primer PCR for the identification of anisakid nematodes from Taiwan Strait. Exp Parasitol 124:197–201PubMedCrossRefGoogle Scholar
  6. Hooper JNA (1983) Parasites of estuarine and oceanic flathead fishes (Family Platycephalidae) from northern New South Wales. Aust J Zool Suppl Ser 90:1–69CrossRefGoogle Scholar
  7. Lèbre C, Petter AJ (1983) Deux nouvelle espèces d’Ascarides (Nematoda) parasites de poisons Téléostéens: Raphidascaris mediterraneus n. sp. et Goezia anguillae n. sp.; complément de description de Cucullanus micropapillatus Tornquist, 1931 (Nematoda, Cucullanidae). Bull Mus Natn d’Hist Nat Paris 4e sér 5(Sect A 2):491–505Google Scholar
  8. Luo D, Huang H (2001) Heliconema minnanensis n. sp. (Physalopteroidea: Physalopteridae) and Raphidascaris trichiuri (Yin and Zhang) n. comb. (Ascaridoidea: Anisakidae) in marine fishes. J Parasitol 87:1090–1094Google Scholar
  9. Moravec F, Justine JL (2005) Two anisakid nematodes from marine fishes off New Caledonia, including Raphidascaris (Ichthyascaris) nemipteri n. sp. from Nemipterus furcosus. Syst Parasitol 62:101–110PubMedCrossRefGoogle Scholar
  10. Moravec F, Nagasawa K (2002) Redescription of Raphidascaris gigi Fujita, 1928 (Nematoda: Anisakidae), a parasite of freshwater fishes in Japan. Syst Parasitol 52:193–198PubMedCrossRefGoogle Scholar
  11. Moravec F, Kohn A, Fernando BMM (1990) First record of Raphidascaris (Sprentascaris) hypostomi (Petter et Cassone, 1984) comb. n. and R. (S.) mahnerti (Petter et Cassone, 1984) comb. n. (Nemat0da:Anisakidae) from Brazil, with remarks on the taxonomic status of the genus Sprentascaris Petter et Cassone, 1984. Folia Parasitol 37:131–140PubMedGoogle Scholar
  12. Olsen LS (1952) Some nematodes parasitic in marine fishes. Publ Inst Mar Sci Univ Tex 2:173–215Google Scholar
  13. Petter AJ, Cassone J (1984) Nematodes de poissons du Paraguay; I. Ascaridoidea: Sprentascaris, n. gen. Rev Suis Zool 91:617–634Google Scholar
  14. Ramallo G (2009) A new species of Raphidascaris (Sprentascaris) (Nematoda: Anisakidae) in Hypostomus cordovae (Pisces: Loricariidae) from Argentina. Zootaxa 2045:60–64Google Scholar
  15. Santos E (1970) Nova espécie do gênero Raphidascaris, Railliet and Henry, 1915 (Nematoda, Ascaridoidea). Atas Soc Biol Rio de Janeiro 14:39–40Google Scholar
  16. Smith JD (1984) Taxonomy of Raphidascaris spp. (Nematoda, Anisakidae) of fishes, with a redescription of R. acus (Bloch, 1772). Can J Zool 62:685–694CrossRefGoogle Scholar
  17. Testini G, Papini R, Lia RP, Parisi A, Dantas-Torres F, Traversa D, Otranto D (2011) New insights into the morphology, molecular characterization and identification of Baylisascaris transfuga (Ascaridida, Ascarididae). Vet Parasitol 175:97–102PubMedCrossRefGoogle Scholar
  18. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680Google Scholar
  19. Wu HW (1949) A note on two parasitic nematodes of fishes. Sinensia 20:51–57Google Scholar
  20. Yamaguti S (1935) Studies on the helminth fauna of Japan. Part 9. Nematodes of fishes. 1. Jpn J Zool 6:337–386Google Scholar
  21. Yin WZ, Zhang NX (1983) On two new species of parasitic nematodes from marine fishes from Zhoushan Islands, China. Acta Zootaxonomic Sinica 8:7–10Google Scholar
  22. Zhang LP, Hu M, Shamsi S, Beveridge I, Li HM, Xu Z, Li L, Cantacessi C, Gasser RB (2007) The specific identification of anisakid larvae from fishes from the Yellow Sea, China, using mutation scanning-coupled sequence analysis of nuclear ribosomal DNA. Mol Cell Probe 21:386–390CrossRefGoogle Scholar
  23. Zhu XQ, Podolska M, Liu JS, Yu HQ, Chen HH, Lin ZX, Luo CB, Song HQ, Lin RQ (2007) Identification of anisakid nematodes with zoonotic potential from Europe and China by single-strand conformation polymorphism analysis of nuclear ribosomal DNA. Parasitol Res 101:1703–1707PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Liang Li
    • 1
  • Yuan-Yuan Liu
    • 1
  • Bao-Chen Liu
    • 2
  • Lu-Ping Zhang
    • 1
    Email author
  1. 1.College of Life ScienceHebei Normal UniversityShijiazhuangPeople’s Republic of China
  2. 2.Botou Vocational CollegeBotouPeople’s Republic of China

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