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Parasitology Research

, Volume 110, Issue 4, pp 1381–1392 | Cite as

Antileishmanial, antitrypanosomal, and cytotoxic screening of ethnopharmacologically selected Peruvian plants

  • Azucena González-Coloma
  • Matías Reina
  • Claudia Sáenz
  • Rodney Lacret
  • Lastenia Ruiz-Mesia
  • Vicente J. Arán
  • Jesús Sanz
  • Rafael A. Martínez-DíazEmail author
Original Paper

Abstract

Extracts (34) from eight plant species of the Peruvian Amazonia currently used in traditional Peruvian medicine, mostly as antileishmanial remedies and also as painkiller, antiseptic, antipyretic, anti-inflamatory, antiflu, astringent, diuretic, antipoison, anticancerous, antiparasitic, insecticidal, or healing agents, have been tested for their antileishmanial, antitrypanosomal, and cytotoxic activity. Plant species were selected based on interviews conducted with residents of rural areas. The different plant parts were dried, powdered, and extracted by maceration with different solvents (hexane, chloroform, and 70% ethanol–water). These extracts were tested on promastigote forms of Leishmania infantum strain PB75, epimastigote forms of Trypanosoma cruzi strain Y, and the mammalian CHO cell line. Parasite viability and nonspecific cytotoxicity were analyzed by a modified MTT colorimetric assay method. The isolation and identification of pure compounds from selected extracts were performed by column chromatography, gas chromatography mass spectrometry (GC-MS; mixtures), spectroscopic techniques [MS, infrared (IR), ultraviolet (UV)], and mono and two-dimensional 1H and 13C nuclear magnetic resonance (NMR; COSY, HSQC, NOESY) experiments. Chondodendron tomentosum bark and Cedrela odorata were the most active extracts against Leishmania, while C. odorata and Aristoloquia pilosa were the most active against Trypanosoma, followed by Tabebuia serratifolia, Tradescantia zebrina, and Zamia ulei. Six compounds and two mixtures were isolated from Z. ulei [cycasin (1)], T. serratifolia {mixtures 1–2, and naphthoquinones 2-acetyl-4H,9H-naphtho[2,3-b]furan-4,9-dione (2) and 2-(1-hydroxyethyl)-4H,9H-naphtho[2,3-b]furan-4,9-dione (3)}, and C. tomentosum [chondrocurine (4); (S,S′)-12-O-methyl(+)-curine (5); and cycleanine (6)]. Four compounds and the two mixtures exhibited significant activity.

Keywords

Visceral Leishmaniasis Leishmaniasis Trypanosoma Naphthoquinones Miltefosine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by grant CTQ2009-14629-C02-01 (Spain) and a collaborative research project CSIC-CONCYTEC (Perú). We also thank S. Carlin for language revision.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Azucena González-Coloma
    • 1
  • Matías Reina
    • 2
  • Claudia Sáenz
    • 1
  • Rodney Lacret
    • 3
  • Lastenia Ruiz-Mesia
    • 4
  • Vicente J. Arán
    • 5
  • Jesús Sanz
    • 6
  • Rafael A. Martínez-Díaz
    • 7
    Email author
  1. 1.Instituto de Ciencias AgrariasConsejo Superior de Investigaciones CientíficasMadridSpain
  2. 2.Instituto de Productos Naturales y AgrobiologíaConsejo Superior de Investigaciones CientíficasLa LagunaSpain
  3. 3.Grupo de Alelopatía, Departamento de Química Orgánica, Facultad de CienciasUniversidad de CádizCadizSpain
  4. 4.Laboratorio de Productos Naturales Antiparasitarios de la Amazonía PeruanaUniversidad Nacional de la Amazonía PeruanaSan Juan BautistaPeru
  5. 5.Instituto de Química MédicaConsejo Superior de Investigaciones CientíficasMadridSpain
  6. 6.Instituto de Química Orgánica GeneralConsejo Superior de Investigaciones CientíficasMadridSpain
  7. 7.Departamento de Medicina Preventiva, Salud Pública y Microbiología, Facultad de MedicinaUniversidad Autónoma de MadridMadridSpain

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