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Parasitology Research

, Volume 110, Issue 4, pp 1347–1355 | Cite as

Necrotic enteritis due to simultaneous infection with Isospora suis and clostridia in newborn piglets and its prevention by early treatment with toltrazuril

  • Heidrun Mengel
  • Monika Kruger
  • Maxie U. Kruger
  • Bernhard Westphal
  • Alexander Swidsinski
  • Sandra Schwarz
  • Hans-Christian Mundt
  • Katja Dittmar
  • Arwid DaugschiesEmail author
Original Paper

Abstract

In this study, 51 piglets originating from five different sows were included in the investigations. The animal source of all sows had a history of Clostridium perfringens type A (β2) infection. The piglets of three sows (n = 31) were experimentally infected with Isospora suis within the first 4 h after birth and were randomly assigned to the treatment group or the sham-dosing group. The piglets of the two remaining sows (n = 20) served as I. suis-uninfected controls. Twelve hours post-infection, the animals in the treatment group (n = 15) were treated with toltrazuril (20 mg/kg BW, Baycox® 5% suspension). During an observation period of 14 days faecal consistency, faecal oocyst counts, faecal germ counts, mortality, body weight development and clinical status were recorded. One piglet per study group and litter was necropsied, and intestinal tissue samples were taken for histopathological investigations and in situ hybridisation on study days (SDs) 3 and 14. I. suis-infected but untreated piglets showed clinical disease resulting in liquefaction of faeces and decreased body weight development. In 59.2% of the observations, I. suis-infected but untreated piglets showed abnormal faecal consistencies whereas only 12.0% or respectively 4.4% of the faecal samples had a pasty consistency in the I. suis-infected–treated or in the control animals. The mean body weight at the end of the study was 3.37 kg in the I. suis-infected but untreated piglets while the average body weight in the I. suis-infected–treated animals was calculated as 4.42 kg and the control animal’s mean body weight was 4.45 kg. Moreover, mortality, occurring between SDs 8 and 14, in this study group was 38.5% (n = 5), with 30.8% (n = 4) died from necrotic enteritis. In contrast, no piglets died in the I. suis-uninfected control group or in the toltrazuril-treated study group. The results of this study corroborate the hypothesis that simultaneous infection with I. suis and C. perfringens type A soon after birth leads to distinct interactions between the two pathogens and result in an increase in clinical disease, mortality and metabolically active C. perfringens type A.

Keywords

Coccidiosis Newborn Piglet Toltrazuril Necrotic Enteritis Oocyst Excretion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

Special thanks to Yvonne Kuhnert for the detection of excreted I. suis oocysts via epifluorescence microscopy.

References

  1. Amann RI, Binder BJ, Olson RJ, Chisholm SW, Devereux RStahl DA (1990) Combination of 16s rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56:1919-1925Google Scholar
  2. Amann RI, Ludwig W, Schleifer K-H (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143–169PubMedGoogle Scholar
  3. Baba E, Ikemoto T, Fukata T, Sasai K, Arakawa A, McDougald LR (1997) Clostridial population and the intestinal lesions in chickens infected with Clostridium perfringens and Eimeria necatrix. Vet Microbiol 54:301–308PubMedCrossRefGoogle Scholar
  4. Bach U, Kalthoff V, Mundt HC, Popp A, Rinke M, Daugschies A, Luttge B (2003) Parasitological and morphological findings in porcine isosporosis after treatment with symmetrical triazintriones. Parasitol Res 91:27–33PubMedCrossRefGoogle Scholar
  5. Collier CT, Hofacre CL, Payne AM, Anderson DB, Kaiser P, Mackie RI, Gaskins HR (2008) Coccidia-induced mucogenesis promotes the onset of necrotic enteritis by supporting Clostridium perfringens growth. Vet Immunol Immunopath 122:104–115CrossRefGoogle Scholar
  6. Daugschies A, Bialek R, Joachim A, Mundt HC (2001) Autofluorescence microscopy for detection of nematode eggs and protozoa, in particular Isospora suis, in swine feces. Parasitol Res 87:409–412PubMedCrossRefGoogle Scholar
  7. Franks AH, Harmsen HJ, Raangs GC, Jansen GJ, Schut F, Welling GW (1998) Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group specific 16s rRNA-targeted oligonucleotide probes. Appl Environ Microbiol 64:3336-3345Google Scholar
  8. Harleman JH, Meyer RC (1984) Life cycle of Isospora suis in gnotobiotic and conventionalized piglets. Vet Parasitol 17(1):27–39PubMedCrossRefGoogle Scholar
  9. Harleman JH, Meyer RC (1985) Pathogenicity of Isospora suis in gnotobiotic and conventionalised piglets. Vet Rec 116(21):561–565PubMedCrossRefGoogle Scholar
  10. Krüger M, Schroedl W, Krüger M, Schwarz S, Mengel H, Daugschies A, Swidsinski A, Mundt HC, Westphal B (2010) Prakt Tierarzt 91:774–784Google Scholar
  11. Kuhnert Y, Schmäschke R, Daugschies A (2006) Comparison of different methods for examining the feces of suckling piglets for Isospora suis. Berl Munch Tierarztl Wochenschr 119(7–8):282–286PubMedGoogle Scholar
  12. Maes D, Vyt P, Rabaeys P, Gevaert D (2007) Effects of toltrazuril on the growth of piglets in herds without clinical isosporosis. Vet J 173:197–199PubMedCrossRefGoogle Scholar
  13. Mavromatis I, Kyriakis CS, Alexopoulos C, Kritas SK, Tzika ED, Kyriakis SC. Efficacy and cost benefit study on the use of toltrazuril for the control of neonatal coccidiosis in pigs due to Isospora suis under field conditions. In: Proceedings of the 18th Congress of the International Pig Veterinary Society, Hamburg, Germany, 2004, 323Google Scholar
  14. McDonel JL (1986) Toxins of Clostridium perfringens type A, B, C, D and E. In: Dorner F, Drews H (eds) Pharmacology of bacterial toxins. Pergamon, Oxford, pp 477–517Google Scholar
  15. Mundt HC, Joachim A, Daugschies A, Zimmermann M (2003) Population biology studies on Isospora suis in piglets. Parasitol Res 90:158–159CrossRefGoogle Scholar
  16. Mundt HC, Joachim A, Becka M, Daugschies A (2006) Isospora suis: an experimental model for mammalian intestinal coccidiosis. Parasitol Res 98:167–175PubMedCrossRefGoogle Scholar
  17. Mundt HC, Mundt-Wüstenberg S, Daugschies A, Joachim A (2007) Efficacy of various anticoccidials against experimental porcine neonatal isosporosis. Parasitol Res 100:401–411PubMedCrossRefGoogle Scholar
  18. Niestrath M, Takla M, Joachim A, Daugschies A (2002) The role of Isospora suis as a pathogen in conventional piglet production in Germany. J Vet Med B Infect Dis Vet Public Health 49(4):176–180PubMedCrossRefGoogle Scholar
  19. Poulsen LK, Ballard G, Stahl DA (1993) Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl Environ Microbiol 59:1354–1360PubMedGoogle Scholar
  20. Robinson Y, Morin M, Girard C, Higgins R (1983) Experimental transmission of intestinal coccidiosis to piglets: clinical, parasitological and pathological findings. Can J Comp Med 47:401–407PubMedGoogle Scholar
  21. Songer JG (1996) Clostridial enteric disease of domestic animals. Clin Microbiol Rev 9:216–234PubMedGoogle Scholar
  22. Songer JG, Anderson MA (2006) Clostridium difficile: an important pathogen of food animals. Anaerobe 12(1):1–4PubMedCrossRefGoogle Scholar
  23. Songer JG, Uzal FA (2005) Clostridial enteric infections in pigs. J Vet Diagn Invest 17(6):528–536PubMedCrossRefGoogle Scholar
  24. Stuart BP, Lindsay DS, Ernst JV, Gosser HS (1980) Isospora suis enteritis in piglets. Vet Pathol 17(1):84–93PubMedGoogle Scholar
  25. Swidsinski A, Loening-Baucke V, Lochs H, Hale LP (2005) Spatial organization of bacterial flora in normal and inflamed intestine: a fluorescence in situ hybridization study in mice. World J Gastroenterol 11:1131–1140PubMedGoogle Scholar
  26. Vítovec J, Koudela B (1990) Double alteration of the small intestine in conventional and gnotobiotic piglets experimentally infected with the coccidium Isospora suis (Apicomplexa, Eimeriidae). Folia Parasitol (Praha) 37(1):21–33Google Scholar
  27. Westphal B, Bernemann U, Kathmann L (2007) Isospora suis and Clostridium perfringens as mixed infection in suckling piglets just after post partum? Tierärztl Umschau 62:682Google Scholar
  28. Williams RB, Marshall RN, La Regione RM, Catchpole J (2003) A new method for the experimental production of necrotic enteritis and its use for studies on the relationship between necrotic enteritis, coccidiosis and anticoccidial vaccination of chickens. Parasitol Res 90:19–26PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Heidrun Mengel
    • 1
    • 2
  • Monika Kruger
    • 1
  • Maxie U. Kruger
    • 1
  • Bernhard Westphal
    • 3
  • Alexander Swidsinski
    • 4
  • Sandra Schwarz
    • 1
  • Hans-Christian Mundt
    • 5
  • Katja Dittmar
    • 1
  • Arwid Daugschies
    • 1
    • 2
    Email author
  1. 1.Centre for Infectious Diseases, Faculty of Veterinary MedicineUniversity of LeipzigLeipzigGermany
  2. 2.Coordination Staff for Veterinary Clinical Studies, Faculty of Veterinary MedicineUniversity of LeipzigLeipzigGermany
  3. 3.Bayer VitalLeverkusenGermany
  4. 4.Laboratory for Molecular Genetics, Polymicrobial Infections and Bacterial Biofilms, Charité HospitalHumboldt UniversityBerlinGermany
  5. 5.Bayer HealthCare GmbHLeverkusenGermany

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