Parasitology Research

, Volume 110, Issue 3, pp 1097–1102 | Cite as

Anthelmintic effect of a methanol extract of Bombax malabaricum leaves on Paramphistomum explanatum

  • Emdad Hossain
  • Goutam Chandra
  • Anadi P. Nandy
  • Subhash C. Mandal
  • Jayanta Kumar Gupta
Original Paper


Bombax malabaricum (family Bombacaceae) is used as anthelmintic in traditional system of medicine in Southern Punjab of Pakistan. The objective of this study was to evaluate the anthelmintic activity of the methanol extract of B. malabaricum leaves (MEBM). Live parasites (trematode: Paramphistomum explanatum) were collected from buffalo in 0.9% phosphate-buffered saline. It was incubated in Petri dishes at 37 ± 1°C in media containing either no extract (control) or MEBM, the test drug at 10, 25, 50, and 100 mg/ml dose level or albendazole, the standard drug at 10 mg/ml. The efficacy of the extract or albendazole was measured on the basis of the loss of spontaneous movement and/or death of the trematodes. Paralysis was considered when there is no movement unless shaken vigorously. Death was confirmed when the trematodes completely lost their motility, even when vigorously shaken or dipped in warm water (50°C), followed by fading away of their body color. The trematodes, both drug treated and others, were further processed for SEM study using the standard method. All trematodes died with all the above-mentioned doses of MEBM within a short period of time (less than 45 min) which was statistically highly significant (p < 0.001). MEBM at 100 mg/ml showed maximum efficacy. It paralyzed and killed trematodes in 18.50 ± 0.62 and 22.17 ± 0.48 min, respectively. SEM study showed that MEBM-treated trematodes were stretched. The study established the anthelmintic activity of MEBM.


Rectal Prolapse Albendazole Scanning Electron Microscope Study Lupeol Spontaneous Movement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are thankful to Mr. Debobrata Devbhuti, Jadavpur University, Kolkata; Mr. Amit Kumar, Pharmacy College, Azamgarh; and Dr. Srikanta Bhattacharya of USIC & CIF, Burdwan University, for their technical help.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Emdad Hossain
    • 1
  • Goutam Chandra
    • 2
  • Anadi P. Nandy
    • 2
  • Subhash C. Mandal
    • 3
  • Jayanta Kumar Gupta
    • 3
  1. 1.Pharmacy CollegeAzamgarhIndia
  2. 2.Parasitology Laboratory, Department of ZoologyThe University of BurdwanBurdwanIndia
  3. 3.Department of Pharmaceutical TechnologyJadavpur UniversityKolkataIndia

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