Anthelmintic effect of a methanol extract of Bombax malabaricum leaves on Paramphistomum explanatum
- 259 Downloads
Bombax malabaricum (family Bombacaceae) is used as anthelmintic in traditional system of medicine in Southern Punjab of Pakistan. The objective of this study was to evaluate the anthelmintic activity of the methanol extract of B. malabaricum leaves (MEBM). Live parasites (trematode: Paramphistomum explanatum) were collected from buffalo in 0.9% phosphate-buffered saline. It was incubated in Petri dishes at 37 ± 1°C in media containing either no extract (control) or MEBM, the test drug at 10, 25, 50, and 100 mg/ml dose level or albendazole, the standard drug at 10 mg/ml. The efficacy of the extract or albendazole was measured on the basis of the loss of spontaneous movement and/or death of the trematodes. Paralysis was considered when there is no movement unless shaken vigorously. Death was confirmed when the trematodes completely lost their motility, even when vigorously shaken or dipped in warm water (50°C), followed by fading away of their body color. The trematodes, both drug treated and others, were further processed for SEM study using the standard method. All trematodes died with all the above-mentioned doses of MEBM within a short period of time (less than 45 min) which was statistically highly significant (p < 0.001). MEBM at 100 mg/ml showed maximum efficacy. It paralyzed and killed trematodes in 18.50 ± 0.62 and 22.17 ± 0.48 min, respectively. SEM study showed that MEBM-treated trematodes were stretched. The study established the anthelmintic activity of MEBM.
KeywordsRectal Prolapse Albendazole Scanning Electron Microscope Study Lupeol Spontaneous Movement
We are thankful to Mr. Debobrata Devbhuti, Jadavpur University, Kolkata; Mr. Amit Kumar, Pharmacy College, Azamgarh; and Dr. Srikanta Bhattacharya of USIC & CIF, Burdwan University, for their technical help.
- Agaie BM, Onyeyili PA (2007) Anthelmintic activity of the crude aqueous leaf extracts of Anogeissus leiocarpus in sheep. Afr J Biotech 6(13):1511–1515Google Scholar
- Anonymous (1988) Wealth of India, volume II, New Delhi. Publication and Information Directorate, CSIR, New Delhi, pp 177–185Google Scholar
- Anonymous (2000) Wealth of India, volume I, first supplement. Publication and Information Directorate, CSIR, New Delhi, p 145Google Scholar
- Balamurugan G, Selvarajan S (2009) Preliminary phytochemical screening and anthelmintic activity of Indigofera tinctoria Linn. Int J Drug Dev Res 1(1):157–160Google Scholar
- Hossain E, Mandal SC, Gupta JK (2011a) Phytochemical screening and in vivo antipyretic activity of the methanol leaf-extract of Bombax malabaricum DC (Bombacaceae). Trop J Pharm Res 10(1):55–60Google Scholar
- Hossain E, Chandra G, Nandy AP, Mandal SC, Gupta JK (2011b) Anthelmintic effect of a methanol extract of leaves of Dregea volubilis on Paramphistomum explanatum. Parasitol Res (in press)Google Scholar
- Lal SS (2006) A text book of practical zoology invertebrate, 9th edn. Rastogi Publications, Meerut, pp 271–272Google Scholar
- Roy B, Lalchhandama K, Dutta BK (2008) Scanning electron microscopic observations on the in vitro anthelmintic effects of Millettia pachycarpa on Raillietina echinobothrida. Phcog Mag 4:20–26Google Scholar
- Seshadri V, Batta AK, Rangaswami S (1971) Phenolic components of Bombax malabaricum. Curr Sci 23:630Google Scholar