Parasitology Research

, Volume 110, Issue 2, pp 971–978 | Cite as

Morphological characterization of adult Fascioloides magna (Trematoda: Fasciolidae): first SEM report

  • Soraya Naem
  • Christine M. Budke
  • Thomas M. Craig
Original Paper

Abstract

Five adult Fascioloides magna specimens were recovered from the livers of naturally infected calves from Texas, USA. Scanning electron microscopy was used to study the morphological characteristics of the trematodes. These mature flukes measured 35–100 mm in length by 15–25 mm in width and had an oval dorsoventrally flattened body, with no anterior cone. The tegument was armed with sharp spines. Around the oral and ventral suckers, some of the spines were small, with a sharp point, while others had serrated edges with 15–22 sharp points. The surface of the oral sucker was covered by an interesting pattern of tegument, small dome-shaped and ciliated papillae. The ventral sucker showed a smooth surface and two unknown spine-like structures. There were fewer spines at the base of the genital pore than on other parts of the anterior end of the worm. At the anterior end of the ventral side, well-developed spines were observed, while at the posterior end of the ventral side, the spines were small, mostly with one or three points and blunted edges. At the posterior end of the dorsal side, the spines became progressively fewer, smaller, and shorter. Around the excretory pore, the tegument was folded, with no spines, and small groups of dome-shaped and ciliated papillae were present. The cirrus organ showed a smooth surface, with small pores on the dorsal side and small groups of tiny spines between the folds. The eggs measured 168 × 101 μm and had a protoplasmic appendage at the pole opposite the operculum. At the posterior end of the dorsal side, and toward the right, a pore with a very thin rim was present, which could be the terminus of Laurer’s canal.

Notes

Acknowledgments

We heartily thank members of the Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, for providing the specimens of F. magna. Also, we would like to express our gratitude to Professor Larry A. Arsenault, head of the electron microscope facility, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada, for his assistance in providing access to SEM facilities during the study. In addition, we would like to acknowledge the valuable help of Mr. Ernie Spitzer, Chief Technician, and all of the technicians in the electron microscope facility. Lastly, we appreciate our colleague Dr. Saeed Maslak for his great assistance in editing the SEM images.

References

  1. Bassi R (1875) Sulla cachessia ittero-verminosa, o marciaia, causata dal Distomum magnum. J Med Vet di Torino 4:497–515Google Scholar
  2. Bazsalovicsova E, Kralova-Hromadova I, Spakulova M, Reblanova M, Oberhauserova K (2010) Determination of ribosomal internal transcribed spacer 2 (ITS2) interspecific markers in Fasciola hepatica, Fascioloides magna, Dicrocoelium dendriticum and Paramphistomum cervi (Trematoda), parasites of wild and domestic ruminants. Helminthologia 47(2):76–82CrossRefGoogle Scholar
  3. Coil WH (1977) The penetration of Fascioloides magna miracidia into the snail host Fossaria bulimoides. A scanning electron microscope study. Parasitol Res 52(1):53–59Google Scholar
  4. Coil WH (1981) Miracidial penetration in Fascioloides magna (Trematoda). Parasitol Res 65(3):299–307Google Scholar
  5. Dangprasert T, Khawsuk W, Meepool A, Wanichanon C, Viyanant V, Upatham ES, Wongratanacheevin S, Sobhon P (2001) Fasciola gigantica: surface topography of the adult tegument. J Helminthol 75(1):43–50PubMedCrossRefGoogle Scholar
  6. Foreyt WJ (1990) Domestic sheep as a rare definitive host of the large American liver fluke Fascioloides magna. J Parasitol 76(5):736–739PubMedCrossRefGoogle Scholar
  7. Fried B, Vates TS, Wisnewski N, Stromberg BE (1986) Scanning electron microscopy and chemical excystation of Fascioloides magna (Trematoda) metacercariae. Parasitol Res 72(5):631–634Google Scholar
  8. Kralova-Hromadova I, Spakulova M, Horackova E, Turcekova L, Novobilsky A, Beck R, Koudela B, Marinculic A, Rajsky D, Pybus M (2008) Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. J Parasitol 94:58–67PubMedCrossRefGoogle Scholar
  9. Mahmoud LH, Hegazi MA (2007) The surface ultrastructure of the Egyptian liver fluke Fasciola gigantica. J Egypt Soc Parasitol 37(2):631–640PubMedGoogle Scholar
  10. Novobilsky A, Kasny M, Mikes L, Kovarcik K, Koudela B (2007) Humoral immune responses during experimental infection with Fascioloides magna and Fasciola hepatica in goats and comparison of their excretory/secretory products. Parasitol Res 101:357–364PubMedCrossRefGoogle Scholar
  11. Qureshi T, Craig TM, Drawe DL, Davis DS (1989) Efficacy of triclabendazole against fascioloidiasis (Fascioloides magna) in naturally infected white-tailed deer (Odocoileus virginianus). J Wildl Dis 25(3):378–383PubMedGoogle Scholar
  12. Radvansky JE, Bazsalovicsova I, Kralova-Hromadova I, Minarik G, Kadasi L (2011) Development of high-resolution melting (HRM) analysis for population studies of Fascioloides magna (Trematoda: Fasciolidae), the giant liver fluke of ruminants. Parasitol Res 108:201–209PubMedCrossRefGoogle Scholar
  13. Reblanova M, Spakulova M, Orosova M, Bazsalovicsova E, Rajsky D (2010) A description of karyotype of the giant liver fluke Fascioloides magna (Trematoda, Platyhelminthes) and a review of Fasciolidae cytogenetics. Helminthol 47(2):69–75CrossRefGoogle Scholar
  14. Soulsby EJL (1986) Helminths, arthropods and protozoa of domesticated animals. Bailliere Tindall, LondonGoogle Scholar
  15. Srimuzipo P, Komalamisra C, Choochote W, Jitpakdi A, Vanichthanakorn P, Keha P, Riyong D, Sukontasan K, Komalamisra N, Sukontasan K, Tippawangkosol P (2000) Comparative morphometry, morphology of egg and adult surface topography under light and scanning electron microscopies, and metaphase karyotype among three size-races of Fasciola gigantica in Thailand. Southeast Asian J Trop Med Public Health 31(2):366–373PubMedGoogle Scholar
  16. Ursprung J, Joachim A, Prosl H (2006) Epidemiology and control of the giant liver fluke, Fascioloides magna, in a population of wild ungulates in the Danubian wetlands east of Vienna. Berliner und Muncher Tiermedicine Woch 119:316–323Google Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Soraya Naem
    • 1
  • Christine M. Budke
    • 2
  • Thomas M. Craig
    • 3
  1. 1.Department of Pathobiology, Faculty of Veterinary MedicineUrmia UniversityUrmiaIran
  2. 2.Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical SciencesTexas A&M UniversityCollege StationUSA
  3. 3.Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical SciencesTexas A&M UniversityCollege StationUSA

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