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First report on susceptibility of wild Aedes aegypti (Diptera: Culicidae) using Carapa guianensis (Meliaceae) and Copaifera sp. (Leguminosae)

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Abstract

Oils of Carapa guianensis and Copaifera spp. are well known in the Amazonian region as natural insect repellent, and studies have reported their efficiency as larvicide against some laboratory mosquito species. However, in wild populations of mosquitoes, these oils have not yet been evaluated. Thus, the objective of this study was to investigate their efficiency as larvicide in wild populations of Aedes aegypti with a history of exposure to organophosphate. The susceptibility of larvae was determined under three different temperatures, 15°C, 20°C, and 30°C. For each test, 1,000 larvae were used (late third instar and early fourth instar—four replicates of 25 larvae per concentration). Statistical tests were used to identify significant differences. The results demonstrated that as the laboratory A. aegypti, the wild populations of A. aegypti were also susceptible to C. guianensis and Copaifera sp. oils. The lethal concentrations for Copaifera sp. ranged from LC50 47 to LC90 91 (milligrams per liter), and for C. guianensis, they were LC50 136 to LC90 551 (milligrams per liter). In relation to different temperature, the effectiveness of the oils on larvae mortality was directly related to the increase of temperature, and better results were observed for temperature at 25°C. The results presented here indicate the potential larvicidal activity of C. guianensis and species of Copaifera, in populations of A. aegypti from the wild. Therefore, the results presented here are very important since such populations are primarily responsible for transmitting the dengue virus in the environment.

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Acknowledgments

The authors thank the Health Department of Parana State, in particular the coordinator of the Entomology, Allan Martins, for his cooperation in sending the material for bioassays and the National Council of Research and Development (CNPq) for scholarships and financial supports (410579/2006-8).

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Authors and Affiliations

  1. Setor de Ciências Biológicas, Departamento de Zoologia, Programa de Pós-graduação em Entomologia, Universidade Federal do Paraná, Caixa Postal 19020, CEP: 81531-980, Curitiba, PR, Brazil

    Josiane S. Prophiro, Mario Antonio Navarro da Silva & Jonny E. Duque-Luna

  2. Grupo de Pesquisa em Imunoparasitologia-IMPAR, Universidade do Sul de Santa Catarina, Av. José Acácio Moreira 787, Dehon, 88704-900, Tubarão, SC, Brazil

    Josiane S. Prophiro & Louyse Caroline B. P. da Rocha

  3. Grupo de Pesquisa em Tecnologia Farmacêutica-TECFARMA, Universidade do Sul de Santa Catarina, Av. José Acácio Moreira 787, Dehon, 88704-900, Tubarão, SC, Brazil

    Luiz A. Kanis

  4. Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, ICBS, Rua Sarmento Leite, 500, sala 206, CEP: 90050-170, Porto Alegre, RS, Brazil

    Onilda S. da Silva

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  1. Josiane S. Prophiro
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  2. Mario Antonio Navarro da Silva
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  3. Luiz A. Kanis
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  4. Louyse Caroline B. P. da Rocha
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  5. Jonny E. Duque-Luna
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  6. Onilda S. da Silva
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Correspondence to Onilda S. da Silva.

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Prophiro, J.S., da Silva, M.A.N., Kanis, L.A. et al. First report on susceptibility of wild Aedes aegypti (Diptera: Culicidae) using Carapa guianensis (Meliaceae) and Copaifera sp. (Leguminosae). Parasitol Res 110, 699–705 (2012). https://doi.org/10.1007/s00436-011-2545-7

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  • DOI: https://doi.org/10.1007/s00436-011-2545-7

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