Parasitology Research

, Volume 110, Issue 1, pp 479–481 | Cite as

Molecular characterization of Cryptosporidium in children in Oyo State, Nigeria: implications for infection sources

  • Adekunle Bamidele Ayinmode
  • Benjamin Olakunle Fagbemi
  • Lihua Xiao
Short Communication

Abstract

A study was conducted to detect and identify Cryptosporidium spp. in 43 children from Oyo State, Nigeria. Using nested polymerase chain reaction, 11.6% of the children were identified as positive for Cryptosporidium spp. Restriction fragment length polymorphism analysis and DNA sequencing of the PCR products showed the presence of three subtype families of Cryptosporidium hominis (two isolates of Ia and one isolate of Ib) and Cryptosporidium parvum (two isolates of IIc), all anthroponotic in nature. This study identified a high diversity of Cryptosporidium subtypes and clearly suggested that anthroponotic rather than zoonotic transmission played a more important role in the epidemiology of Cryptosporidium in the studied area.

Notes

Acknowledgements

This work was supported by the John D. and Catherine T. MacArthur Foundation Overseas Training Grant from the University of Ibadan (Nigeria) and the Centers for Disease Control and Prevention, Atlanta, GA, USA. We thank Theresa Dearen for technical assistance. This study received approval from the University of Ibadan/University College Hospital Ethics Committee with the assigned number UI/EC/10/0129. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

References

  1. Alves M, Xiao L, Sulaiman IM, Lal AA, Matos O, Antunes F (2003) Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J Clin Microbiol 41:2744–2747PubMedCrossRefGoogle Scholar
  2. Cama VA, Ross JM, Crawford S, Kawai V, Chavez-Valdez R, Vargas D, Vivar A, Ticona E, Navincopa M, Williamson J, Ortega Y, Gilman RH, Bern C, Xiao L (2003) Differences in clinical manifestations among Cryptosporidium species and subtypes in HIV-infected persons. J Infect Dis 169(5):684–691Google Scholar
  3. Cama VA, Bern C, Roberts J, Cabrera L, Sterling CR, Ortega Y, Gilman RH, Xiao L (2008) Cryptosporidium species and subtypes and clinical manifestations in children, Peru. Emerg Infect Dis 14:1567–1574PubMedCrossRefGoogle Scholar
  4. Hunter PR, Thompson RC (2005) The zoonotic transmission of Giardia and Cryptosporidium. Int J Parasitol 35:1181–1190PubMedCrossRefGoogle Scholar
  5. Mallon ME, MacLeod A, Wastling JM, Smith H, Tait A (2003) Multilocus genotyping of Cryptosporidium parvum type 2: population genetics and sub-structuring. Infect Genet Evol 3:207–218PubMedCrossRefGoogle Scholar
  6. Molloy SF, Smith HV, Kirwan P, Nichols RAB, Asaolu SO, Connelly L, Holland CV (2010) Identification of a high diversity of Cryptosporidium species genotypes and subtypes in a pediatric population in Nigeria. Am J Trop Med Hyg 82:608–613PubMedCrossRefGoogle Scholar
  7. Sulaiman IM, Hira PR, Zhou L, Al-Ali FM, Al-Shelahi FA, Shweiki HM (2005) Unique endemicity of cryptosporidiosis in children in Kuwait. J Clin Microbiol 43:2805–2809PubMedCrossRefGoogle Scholar
  8. Xiao L (2010) Molecular epidemiology of cryptosporidiosis: an update. Experimental Parasitol 124:80–89CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Adekunle Bamidele Ayinmode
    • 1
    • 2
  • Benjamin Olakunle Fagbemi
    • 1
  • Lihua Xiao
    • 2
  1. 1.Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary MedicineUniversity of IbadanIbadanNigeria
  2. 2.Division of Foodborne, Waterborne, and Environmental DiseasesCenters for Disease Control and PreventionAtlantaUSA

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