Molecular characterization of Cryptosporidium in children in Oyo State, Nigeria: implications for infection sources
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A study was conducted to detect and identify Cryptosporidium spp. in 43 children from Oyo State, Nigeria. Using nested polymerase chain reaction, 11.6% of the children were identified as positive for Cryptosporidium spp. Restriction fragment length polymorphism analysis and DNA sequencing of the PCR products showed the presence of three subtype families of Cryptosporidium hominis (two isolates of Ia and one isolate of Ib) and Cryptosporidium parvum (two isolates of IIc), all anthroponotic in nature. This study identified a high diversity of Cryptosporidium subtypes and clearly suggested that anthroponotic rather than zoonotic transmission played a more important role in the epidemiology of Cryptosporidium in the studied area.
This work was supported by the John D. and Catherine T. MacArthur Foundation Overseas Training Grant from the University of Ibadan (Nigeria) and the Centers for Disease Control and Prevention, Atlanta, GA, USA. We thank Theresa Dearen for technical assistance. This study received approval from the University of Ibadan/University College Hospital Ethics Committee with the assigned number UI/EC/10/0129. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.
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