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Parasitology Research

, Volume 110, Issue 1, pp 205–210 | Cite as

Ultrastructure, chromosomal karyotype, and molecular phylogeny of a new isolate of microsporidian Vairimorpha sp. BM (Microsporidia, Nosematidae) from Bombyx mori in China

  • Handeng Liu
  • Guoqing Pan
  • Tian Li
  • Wei Huang
  • Bo Luo
  • Zeyang Zhou
Original Paper

Abstract

The spore morphology, chromosomal karyotype, and molecular systematic of a new microsporidian which was isolated from the domesticated silkworm Bombyx mori (Lepidoptera: Bombycidae) in Shandong, China have been studied. The spores were long oval and measured 3.4 × 1.6 μm on fresh smears. Ultrastructure of the spores was characteristic for the genus Vairimorpha: 13–15 polar filament coils, posterior vacuole, and a diplokaryon. Six chromosome bands have been separated by pulsed field gel electrophoresis. The sequenced complete rRNA gene of this isolate is 4,231 bp long. Phylogenetic analysis based on SSU rRNA gene and LSU rRNA gene both revealed that this novel microsporidian which was isolated from B. mori had close relationship to the genus Vairimorpha, not to the genus Nosema. Moreover, the organization of the rRNA units of this microsporidian is not similar to that of Nosema bombycis, but same to that of other microsporidian, such as Vairimorpha necatrix. Although this microsporidian, designed as Vairimorpha sp. BM, was isolated from B. mori, all of these informations indicate that this isolate is closely related to the Vairimorpha group.

Keywords

Chromosomal Band Domesticate Silkworm Microsporidian Species Nosema Species Discontinuous Percoll Density Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors are grateful to senior agronomist Zhaoyi Guo for providing the initial extract of the spore sample. We are grateful to everyone who generously provided us with access to the software used in this work. This work is supported by the National Natural Science Foundation of China under Grant No. 30930067, the Program of Introducing Talents of Discipline to Universities (No. B07045), and the Research Fund of the Doctoral Program of Southwest University under Grant No. swu109039.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Handeng Liu
    • 1
  • Guoqing Pan
    • 1
  • Tian Li
    • 1
  • Wei Huang
    • 1
  • Bo Luo
    • 1
  • Zeyang Zhou
    • 1
    • 2
  1. 1.Institute of Sericulture and Systems BiologySouthwest UniversityChongqingPeople’s Republic of China
  2. 2.Laboratory of Animal BiologyChongqing Normal UniversityChongqingPeople’s Republic of China

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