Parasitology Research

, 109:1267

Toxocara canis larvae reinfecting BALB/c mice exhibit accelerated speed of migration to the host CNS

  • Petra Kolbeková
  • David Větvička
  • Jan Svoboda
  • Karl Skírnisson
  • Markéta Leissová
  • Martin Syrůček
  • Helena Marečková
  • Libuše Kolářová
Original Paper

DOI: 10.1007/s00436-011-2371-y

Cite this article as:
Kolbeková, P., Větvička, D., Svoboda, J. et al. Parasitol Res (2011) 109: 1267. doi:10.1007/s00436-011-2371-y

Abstract

Using a small animal imaging system, migratory activity of Toxocara canis larvae stained by carboxyfluorescein succinimidyl ester (CFSE) was observed post primary infection (PPI) and post reinfection (PR) of BALB/c mice. Each infection was performed with 1,000 larvae per mouse. Primary infections were performed with labeled larvae, while for challenge infections the reinfecting larvae were stained by CFSE. The worm burden in mouse organs was determined during a period from 6 h to 21 days and 4 months PPI and PR. In comparison with primary infections that led to the first larvae appearance in the brain after 60 h, greatly accelerated migration of the parasites administered 3 weeks PPI to the CNS and eyes of challenged mice was noted—in both organs the larvae appeared 6 h PR. In all challenged mice, reinfecting larvae prevailed in the resident parasite population. Preliminary experiments with Toxocara cati larvae also revealed early brain involvement in primarily infected mice. Staining of T. canis larvae by CFSE had no effect on the development of a humoral antibody response against T. canis excretory–secretory antigens. In ELISA, elevated levels of specific IgG and IgG1 were noted on day 14 PPI and the levels of antibodies increased till the end of experiment. Reinfection induced an increase in the levels of both antibodies. In terms of optical density, IgG1 antibodies gave higher values in all sera examined. In ELISA for IgG antibodies, an increase in the avidity index of around 50% was detected 1 month PPI; higher-avidity antibodies were also detected in sera of reinfected animals.

Abbreviations

AI

Avidity index

BBB

Blood–brain barrier

CFSE

Carboxyfluorescein succinimidyl ester

CNS

Central nervous system

DT

Digestion technique

OD

Optical density

PBS

Phosphate-buffered saline

PPI

Post primary infection

PR

Post reinfection

SAIS

Small animal imaging system

TES

Toxocara canis excretory–secretory antigens

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Petra Kolbeková
    • 1
    • 3
  • David Větvička
    • 4
  • Jan Svoboda
    • 4
  • Karl Skírnisson
    • 5
  • Markéta Leissová
    • 1
  • Martin Syrůček
    • 6
  • Helena Marečková
    • 2
  • Libuše Kolářová
    • 1
    • 2
  1. 1.National Reference Laboratory for Tissue HelminthosesGeneral University Hospital in PraguePragueCzech Republic
  2. 2.Institute of Immunology and Microbiology, First Faculty of MedicineCharles University in Prague and General University Hospital in PraguePrague 2Czech Republic
  3. 3.Department of Medical Microbiology, Third Faculty of MedicineCharles University in PraguePragueCzech Republic
  4. 4.Department of Immunology and Gnotobiology, Institute of MicrobiologyAcademy of SciencePragueCzech Republic
  5. 5.Institute of Experimental PathologyUniversity of IcelandKeldurIceland
  6. 6.Department of PathologyNa Homolce HospitalPragueCzech Republic

Personalised recommendations