Parasitology Research

, Volume 109, Issue 4, pp 1075–1084 | Cite as

An evolutionary analysis of trypanosomatid GP63 proteases

  • Lina Ma
  • Kaifu Chen
  • Qingshu Meng
  • Qingyou Liu
  • Petrus Tang
  • Songnian HuEmail author
  • Jun YuEmail author
Original Paper


The trypanosomatid GP63 proteases are known to be involved in parasite–host interaction and exhibit strong sequence and structural similarities to those of their hosts and insect vectors. Based on genome sequences of the three trypanosomatids, Trypanosoma brucei, Trypanosoma cruzi, and Leishmania spp., we annotated all their GP63 proteases and divided highly duplicated T. cruzi GP63 proteases into four novel groups according to sequence features. In Leishmania spp., we studied the evolutionary dynamics of GP63 proteins and identified 57 amino acid sites that are under significant positive selections. These sites may contribute to the functional variations of the GP63 proteases and provide clues for vaccine development.


Visceral Leishmaniasis Leishmaniasis Cutaneous Leishmaniasis Site Model Leishmania Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are very grateful to Drs. Yingfeng Luo and Dan Qi for their useful suggestions and comments during the study and Mr. Joe Yu for editing the manuscript. The work is supported by a grant from National Science and Technology Key Project (2008ZX1004-013), the Ministry of Science and Technology of the People’s Republic of China.

Supplementary material

436_2011_2348_MOESM1_ESM.doc (76 kb)
Resource 1 Abbreviations for all the species used in our analysis (DOC 75 kb)
436_2011_2348_MOESM2_ESM.doc (247 kb)
Resource 2 The GI number and Genbank accession of the amino acid sequences that are used in our analysis (DOC 247 kb)
436_2011_2348_MOESM3_ESM.doc (418 kb)
Resource 3 Redefinition of leishmanolysin based on pfam GP63 model. Each fragment listed in the table is considered to be similar to pfam’s leishmanolysin model (DOC 418 kb)
436_2011_2348_MOESM4_ESM.doc (230 kb)
Resource 4 The relative positions of common segment to the zinc-binding motif. “0” means the common segment has been interrupted (DOC 230 kb)
436_2011_2348_MOESM5_ESM.doc (140 kb)
Resource 5 Alignment of the 290 sequences’ common segment. The colored sites are conserved in more than 90% sequences (DOC 140 kb)
436_2011_2348_MOESM6_ESM.doc (757 kb)
Resource 6 The complete maximun likelihood (ML) tree of the 290 GP63 protease (DOC 757 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of GenomicsChinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.Graduate UniversityChinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.Molecular Regulation and Bioinformatics LaboratoryChang Gung UniversityTaoyuanTaiwan
  4. 4.Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bioresource Conservation and UtilizationGuangxi UniversityNanningPeople’s Republic of China

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