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Parasitology Research

, 109:899 | Cite as

Identification of the cross-reactive and species-specific antigens between Neospora caninum and Toxoplasma gondii tachyzoites by a proteomics approach

  • Houshuang Zhang
  • Eung-goo Lee
  • Longzheng Yu
  • Suguru Kawano
  • Penglong Huang
  • Min Liao
  • Osamu Kawase
  • Guohong Zhang
  • Jinlin Zhou
  • Kozo Fujisaki
  • Yoshifumi Nishikawa
  • Xuenan XuanEmail author
Original Paper

Abstract

The characterization of the cross-reactive and species-specific antigens of Neospora caninum and Toxoplasma gondii is important in the exploration to determine the common mechanisms of parasite–host interaction and to improve the serological diagnosis; it is also useful for the selection of the cross-reactive antigens that could be used in the development of vaccines or drugs for controlling the diseases caused by these two parasites. In this study, cross-reactive and species-specific antigens between N. caninum and T. gondii tachyzoites were comprehensively investigated using a proteomics approach with the application of two-dimensional gel electrophoresis, immunoblot analysis, matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF-MS), and MALDI-TOF/TOF-MS analysis. Immunoblotting and mass spectrometry analysis revealed that at least 42 individual protein spots of N. caninum were reacted with the anti-N. caninum serum, among which at least 18 protein spots were cross-reacted with the anti-T. gondii serum. Moreover, at least 31 protein spots of T. gondii were reacted with the anti-T. gondii serum, among which at least 19 protein spots were cross-reacted with the anti-N. caninum serum. Furthermore, some new specific proteins were also identified in the N. caninum protein profile by searching Toxoplasma sequences or sequences from other organisms. This study substantiates the usefulness of proteomics in the immunoscreening of the cross-reactive or species-specific antigens of both parasites. In addition, the present study showed that there was significant homology in the antigenic proteome profiles between the two parasites. These observations have implications for the design of multicomponent common vaccines against both parasite infections.

Keywords

Protein Spot Visceral Leishmaniasis Toxoplasmosis Protein Disulfide Isomerase Potential Vaccine Candidate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by a grant from The 21st Century COE Program (A-1) and a Grant-in-Aid for Scientific Research, both from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Houshuang Zhang
    • 1
    • 2
  • Eung-goo Lee
    • 1
  • Longzheng Yu
    • 1
  • Suguru Kawano
    • 3
  • Penglong Huang
    • 1
  • Min Liao
    • 1
  • Osamu Kawase
    • 1
  • Guohong Zhang
    • 1
  • Jinlin Zhou
    • 1
    • 2
  • Kozo Fujisaki
    • 3
  • Yoshifumi Nishikawa
    • 1
  • Xuenan Xuan
    • 1
    Email author
  1. 1.National Research Center for Protozoan DiseasesObihiro University of Agriculture and Veterinary MedicineObihiroJapan
  2. 2.Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghaiChina
  3. 3.Department of Frontier Veterinary Science, Faculty of AgricultureKagoshima UniversityKagoshimaJapan

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