Parasitology Research

, Volume 109, Issue 1, pp 205–212 | Cite as

Development of a new PCR protocol to detect and subtype Blastocystis spp. from humans and animals

  • Mónica SantínEmail author
  • María Teresa Gómez-Muñoz
  • Gloria Solano-Aguilar
  • Ronald Fayer
Original Paper


Blastocystis spp. is commonly found in the feces of humans worldwide. Infection has been reported as asymptomatic, acute symptomatic, and chronic symptomatic. This wide range of responses to infection could be related to the genetic diversity of morphologically indistinguishable specimens obtained from infected hosts. The former name Blastocystis hominis is now reported as Blastocystis spp. because of its genetic diversity. Blastocystis is recognized as a complex of subtypes that have not been fully characterized as independent species. The finding of Blastocystis spp. in feces from several animal species suggests a zoonotic potential. Based on conserved regions of published nucleotide SSU rDNA sequences from all Blastocystis subtypes found in GenBank, a PCR and sequencing protocol was developed. The ~500 bp SSU rDNA gene fragment amplified by this PCR is highly sensitive compared with published primers and contains highly variable regions that allow phylogenetic analysis of Blastocystis. These primers were used to detect and subtype Blastocystis spp. specimens from naturally infected humans, primates, cattle, pigs, and chickens. Based on these findings, application of this method can elucidate the complexity of this heterogeneous genus and its role in human and animal disease, as well as its zoonotic potential.


Human Specimen Zoonotic Potential Blastocystis Infection Primate Specimen Enterocytozoon Bieneusi 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank Meghan Heffron for her technical services in support of this study.


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Copyright information

© Springer-Verlag (outside the USA) 2011

Authors and Affiliations

  • Mónica Santín
    • 1
    Email author
  • María Teresa Gómez-Muñoz
    • 2
  • Gloria Solano-Aguilar
    • 3
  • Ronald Fayer
    • 1
  1. 1.Environmental Microbial and Food Safety LaboratoryAnimal and Natural Resources Institute, Agricultural Research Service, United States Department of AgricultureBeltsvilleUSA
  2. 2.Departamento de Sanidad Animal, Facultad de VeterinariaUniversidad Complutense de MadridMadridSpain
  3. 3.United States Department of Agriculture, Beltsville Nutrition Research CenterDiet Genomics and Immunology Laboratory, Agricultural Research ServiceBeltsvilleUSA

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