Parasitology Research

, Volume 109, Issue 1, pp 71–79 | Cite as

Involvement of the GP63 protease in infection of Trichomonas vaginalis

  • Lina Ma
  • Qingshu Meng
  • Weihung Cheng
  • Yunju Sung
  • Petrus Tang
  • Songnian HuEmail author
  • Jun YuEmail author
Original Paper


There are 48 members of the GP63 protease family in Trichomonas vaginalis according to our annotations; 37 of them are predicted to be transmembrane proteins. Because the GP63 protease family is the largest surface protease family and the second largest surface protein family, they are most likely to be involved in the interactions between T. vaginalis and the host cell surfaces, or otherwise participate in infection. We performed a preliminary study on the functions of GP63 in T. vaginalis (TvGP63). We demonstrated the cell surface localization of one highly expressed member of TvGP63 using indirect immunofluorescence assays in both isolate T016 and isolate 30236. The specific inhibitor of TvGP63 protease, 1,10-phenanthroline, was found to significantly inhibit the destruction of HeLa cells, whereas another chelator, EDTA, could not. Further tests showed that 1,10-phenanthroline did not inhibit the adherence of T. vaginalis cells to HeLa cells. The results presented in here suggest that GP63 protease plays a vital role in T. vaginalis infection process, but may not be related to the adherence of parasitic cells to their hosts.


HeLa Cell Differential Expression Analysis Indirect Immunofluorescence Assay Parasite Cell Vaginal Epithelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We would like to acknowledge Prof. J. F. Alderete for supplying T016 isolate. We thank Prof. Changqing Zeng, Prof. Zhiyang Dong, Dr. Jie Feng, Mr. Xumin Wang, and Mr. Yanqiang Wang for their help in carrying out certain experiments. We are also grateful to Mr. Joe Yu for his revision of the manuscript. This work is supported by grants from National Science and Technology Key Project (2008ZX1004-013) and 863 Program (2009AA01A130) from the Ministry of Science and Technology of the People’s Republic of China.

Supplementary material

436_2010_2222_MOESM1_ESM.doc (32 kb)
Online Resource 1 HmmPfam annotation results. The segment that is annotated as the part of GP63 protease domain is listed as following (TVAG_271800 has reached the cutoff score, but has no annotation of the protease domain because of less similarity to the GP63 protease domain in pfam) (DOC 32 kb)
436_2010_2222_MOESM2_ESM.doc (104 kb)
Online Resource 2 Basic information of TvGP63 (DOC 104 kb)
436_2010_2222_MOESM3_ESM.doc (70 kb)
Online Resource 3 Antiserum test (DOC 69 kb)
436_2010_2222_MOESM4_ESM.doc (34 kb)
Online Resource 4 Adhered T. vaginalis number in the adherence experiment. The initial number of parasite cell adding to each well is 1.2 × 106 (DOC 34 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.CAS Key Laboratory of Genome Sciences and InformationBeijing Institute of Genomics, Chinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.Graduate University of Chinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.Molecular Regulation and Bioinformatics LaboratoryChang Gung UniversityTaoyuanTaiwan
  4. 4.Institute of Microbiology and ImmunologyMedical CollegeNational Cheng KungTaiwan

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