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Parasitology Research

, Volume 108, Issue 3, pp 693–702 | Cite as

Synthesis of silver nanoparticles using Nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors

  • Thirunavukkarasu Santhoshkumar
  • Abdul Abdul Rahuman
  • Govindasamy Rajakumar
  • Sampath Marimuthu
  • Asokan Bagavan
  • Chidambaram Jayaseelan
  • Abdul Abduz Zahir
  • Gandhi Elango
  • Chinnaperumal Kamaraj
Original Paper

Abstract

The aim of this study was to investigate the larvicidal potential of the hexane, chloroform, ethyl acetate, acetone, methanol, and aqueous leaf extracts of Nelumbo nucifera Gaertn. (Nymphaeaceae) and synthesized silver nanoparticles using aqueous leaf extract against fourth instar larvae of Anopheles subpictus Grassi and Culex quinquefasciatus Say (Diptera: Culicidae). Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by aqueous extract of plant parts to generate extremely stable silver nanoparticles in water. The results recorded from UV–vis spectrum, scanning electron microscopy, X-ray diffraction, and Fourier transform infrared support the biosynthesis and characterization of silver nanoparticles. Larvae were exposed to varying concentrations of plant extracts and synthesized silver nanoparticles for 24 h. All extracts showed moderate larvicidal effects; however, the maximum efficacy was observed in crude methanol, aqueous, and synthesized silver nanoparticles against the larvae of A. subpictus (LC50 = 8.89, 11.82, and 0.69 ppm; LC90 = 28.65, 36.06, and 2.15 ppm) and against the larvae of C. quinquefasciatus (LC50 = 9.51, 13.65, and 1.10 ppm; LC90 = 28.13, 35.83, and 3.59 ppm), respectively. These results suggest that the leaf methanol, aqueous extracts of N. nucifera, and green synthesis of silver nanoparticles have the potential to be used as an ideal eco-friendly approach for the control of the A. subpictus and C. quinquefasciatus. This is the first report on the mosquito larvicidal activity of the plant extracts and synthesized nanoparticles.

Keywords

Silver Nanoparticles Leaf Extract Lymphatic Filariasis Mosquito Larva Betulinic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors are grateful to C. Abdul Hakeem College Management, Dr. S. Mohammed Yousuff, Principal, and Dr. K. Abdul Subhan, HOD of Zoology Department, for providing the facilities to carry out this work. We acknowledge the support extended by Tamil Nadu Veterinary and Animal Sciences University, Veppery, Chennai and Cochin University of Science and Technology, Cochin in analyzing the samples by TEM and SEM.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Thirunavukkarasu Santhoshkumar
    • 1
  • Abdul Abdul Rahuman
    • 1
  • Govindasamy Rajakumar
    • 1
  • Sampath Marimuthu
    • 1
  • Asokan Bagavan
    • 1
  • Chidambaram Jayaseelan
    • 1
  • Abdul Abduz Zahir
    • 1
  • Gandhi Elango
    • 1
  • Chinnaperumal Kamaraj
    • 1
  1. 1.Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of ZoologyC. Abdul Hakeem CollegeMelvisharam, Vellore DistrictIndia

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