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Parasitology Research

, Volume 108, Issue 2, pp 309–315 | Cite as

Characterisation of Sarcoptes scabiei antigens

  • Gloria Hejduk
  • Katja Hofstätter
  • Michael Löwenstein
  • Roman Peschke
  • Ingrid Miller
  • Anja JoachimEmail author
Original Paper

Abstract

In pig herds, the status of Sarcoptes scabiei infections is routinely monitored by serodiagnosis. Crude antigen for ELISA is usually prepared from S. scabiei var. canis or other variations and may lead to variations in the outcome of different tests, making assay standardisation difficult. This study was performed to investigate the antigen profiles of S. scabiei, including differences between hydrophilic and more hydrophobic protein fractions, by Western blotting with sera from pigs with defined infection status. Potential cross-reactivity among S. scabiei (var. canis, suis and bovis), Dermatophagoides farinae and Tyrophagus putrescentiae was also analysed. Hydrophobic S. scabiei antigens were detectable in the range of 40–50 kDa, whilst the hydrophilic fraction showed no specific antigenicity. In the hydrophobic fractions of D. farinae and T. putrescentiae, two major protein fractions in a similar size range could be identified, but no cross-reactivity with Sarcoptes-positive sera was detectable. However, examination of the hydrophilic fractions revealed cross-reactivity between Sarcoptes-positive sera and both the house dust mite and the storage mite in the range of 115 and 28/38 kDa. Specific bands in the same range (42 and 48 kDa) could be detected in blots from hydrophobic fractions of all three tested variations of S. scabiei (var. canis, bovis and suis). These results show that there are considerable differences in mange antibody reactivity, including reactions with proteins from free-living mites, which may interfere with tests based on hydrophilic antigens. Further refinement of antigen and the use of specific hydrophobic proteins could improve ELISA performance and standardisation.

Keywords

House Dust House Dust Mite Scabies Positive Seron Sarcoptic Mange 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The strains of the free-living mites D. farinae were a kind gift from Prof. Heinz Mehlhorn, Düsseldorf. The protein sequence determination was conducted at the Max F. Perutz Laboratories Mass Spectrometry Facility, Vienna, Austria.

Supplementary material

436_2010_2063_MOESM1_ESM.doc (33 kb)
ESM 1 (DOC 33 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Gloria Hejduk
    • 1
  • Katja Hofstätter
    • 1
  • Michael Löwenstein
    • 1
  • Roman Peschke
    • 1
  • Ingrid Miller
    • 2
  • Anja Joachim
    • 1
    Email author
  1. 1.Institute of Parasitology, Department of PathobiologyUniversity of Veterinary Medicine ViennaViennaAustria
  2. 2.Medical Chemistry, Department of Biomedical SciencesUniversity of Veterinary Medicine ViennaViennaAustria

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