Advertisement

Parasitology Research

, Volume 107, Issue 4, pp 783–785 | Cite as

Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic

  • Eva BártováEmail author
  • Kamil Sedlák
  • Michaela Syrová
  • Ivan Literák
Original Article

Abstract

During January 2007, blood samples were collected from 552 healthy horses from nine different regions of the Czech Republic. Sera were tested for serum antibodies to Neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay and confirmed by an indirect fluorescent antibody test. The same samples were tested for serum antibodies against Toxoplasma gondii by a latex agglutination test. In total, 131 of 552 (24%) horses reacted positively for Neospora antibodies in competitive-inhibition enzyme-linked immunosorbent assay; seven of them had ≥50% of inhibition. Samples were confirmed in indirect fluorescence test, and only two samples were positive with final titres 50 and 100, while others were negative. Antibodies against T. gondii were found in 125 (23%) horses. This is the first serologic survey for Neospora spp. antibodies performed on horses in the Czech Republic.

Keywords

Czech Republic Toxoplasmosis Definitive Host Toxoplasma Gondii Indirect Fluorescent Antibody Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was supported by the Ministry of Education, Youth and Sports of the Czech Republic (Grant No. MSM6215712402).

References

  1. Akca A, Babür C, Arslan MO, Gicik Y, Kara M, Kilic S (2004) Prevalence of antibodies to Toxoplasma gondii in horses in the province of Kars, Turkey. Vet Med – Czech 49:9–13Google Scholar
  2. Babür C, Cakmak A, Biyikoglu G, Piskin C (1998) The detection of anti-Toxoplasma gondii by Sabin-Feldman dye test in horses which were slaughtered to feed the wild animals in the Zoo of the Atatürk forest farm. Türkiye Parazitol Derg 22:174–176Google Scholar
  3. Cheadle MA, Lindsay DS, Rowe S, Dykstra CC, Williams MA, Spencer JA, Toivio-Kinnucan MA, Lenz SD, Newton JC, Rolsma MD, Blagburn BL (1999) Prevalence of antibodies to Neospora caninum in horses from Alabama and characterisation of an isolate recovered from a naturally infected horse. Int J Parasitol 29:1537–1543CrossRefPubMedGoogle Scholar
  4. Ciaramella P, Corona M, Cortese L, Piantedosi D, Santoro D, Loria A, Rigato R (2004) Seroprevalence of Neospora spp. in asymptomatic horses in Italy. Vet Parasitol 123:11–15CrossRefPubMedGoogle Scholar
  5. Dubey JP (2003) Review of Neospora caninum and neosporosis in animals. Korean J Parasitol 41:1–16CrossRefPubMedGoogle Scholar
  6. Dubey JP, Beattie CP (1988) Toxoplasmosis of animals and man. CRC Press, Boca Raton, FL, p 220Google Scholar
  7. Dubey JP, Romand S, Thulliez P, Kwok OCH, Shen SK, Gamble HR (1999a) Prevalence of antibodies to Neospora caninum in horses in North America. J Parasitol 85:968–969CrossRefGoogle Scholar
  8. Dubey JP, Kerber CE, Granstrom DE (1999b) Serologic prevalence of Sarcocystis neurona, Toxoplasma gondii, and Neospora caninum in horses in Brazil. JAVMA 215:970–972Google Scholar
  9. Dubey JP, Venturini MC, Venturini L, McKinney J, Pecoraro M (1999c) Prevalence of antibodies to Sarcocystis neurona, Toxoplasma gondii and Neospora caninum in horses from Argentina. Vet Parasitol 86:59–62CrossRefGoogle Scholar
  10. Dubey JP, Mitchell SM, Morrow JK, Rhyan JC, Stewart LM, Granstrom DE, Romand S, Thulliez P, Savile WJ, Lindsay DS (2003) Prevalence of antibodies to Neospora caninum, Sarcocystis neurona, and Toxoplasma gondii in wild horses from central Wyoming. J Parasitol 89:716–720CrossRefPubMedGoogle Scholar
  11. Gondim LFP, Lindsay DS, McAllister MM (2009) Canine and bovine Neospora caninum control sera examined using N. caninum and N. hughesi indirect fluorescent antibody tests. J Parasitol 95:86–88CrossRefPubMedGoogle Scholar
  12. Gupta GD, Lakritz J, Kim JH, Kim DY, Kim JK, Marsh AE (2002) Seroprevalence of Neospora, Toxoplasma gondii and Sarcocystis neurona antibodies in horses from Jeju island, South Korea. Vet Parasitol 106:193–201CrossRefPubMedGoogle Scholar
  13. Hejlíček K, Literák I (1994) Prevalence of antibodies to Toxoplasma gondii in horses in the Czech Republic. Acta Parasitol 39:217–219Google Scholar
  14. Jakubek EB, Lundén A, Uggla A (2006) Seroprevalence of Toxoplasma gondii and Neospora sp. infections in Swedish horses. Vet Parasitol 138:194–199CrossRefPubMedGoogle Scholar
  15. Kilbas ZG, Adanir R, Avcioglu H (2008) Seroprevalence of Neospora caninum in racehorshes in Ankara, Turkey. Acta Parasitol 53:315–316CrossRefGoogle Scholar
  16. Kligler EB, Shkap V, Baneth G, Mildenberg Z, Steinman A (2007) Seroprevalence of Neospora spp. among asymptomatic horses, aborted mares and horses demonstrating neurological signs in Israel. Vet Parasitol 148:109–113CrossRefPubMedGoogle Scholar
  17. Lindsay DS (2001) Neosporosis: an emerging protozoal disease of horse. Equine Vet J 33:116–118CrossRefPubMedGoogle Scholar
  18. Locatelli-Ditrich R, Dittrich JR, Richartz RRTB, Gasino Joineau MEG, Antunes J, Pinckney RD, Deconto I, Hoffmann DCS, Thomaz-Soccol V (2006) Investigation of Neospora sp. and Toxoplasma gondii antibodies in mares and in precolostral foals from Parana State, Southern Brazil. Vet Parasitol 135:215–221CrossRefGoogle Scholar
  19. McDole MG, Gay JM (2002) Seroprevalence of antibodies against Neospora caninum in diagnostic equine serum samples and their possible association with fetal loss. Vet Parasitol 105:257–260CrossRefPubMedGoogle Scholar
  20. Pitel PH, Romand S, Pronost S, Foucher N, Gargala G, Maillard K, Thulliez P, Collobert-Laugier C, Tainturier D, Fortier G, Ballet JJ (2003) Investigation of Neospora sp. antibodies in aborted mares from Normandy, France. Vet Parasitol 118:1–6CrossRefPubMedGoogle Scholar
  21. Uggla A, Mattson S, Juntti N (1990) Prevalence of antibodies to Toxoplasma gondii in cats, dogs and horses in Sweden. Acta Vet Scand 31:219–222PubMedGoogle Scholar
  22. Wyss R, Sager H, Müller N (2000) Distribution of Toxoplasma gondii and Neospora caninum under aspects of meat hygiene. Schweiz Arch Tierheilkd 142:95–108PubMedGoogle Scholar
  23. Zástěra M, Hübner J, Pokorný J, Seeman J (1966) Králíci jako možný zdroj toxoplazmické infekce člověka. Čs Epidem 15:172–177Google Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Eva Bártová
    • 1
    Email author
  • Kamil Sedlák
    • 2
  • Michaela Syrová
    • 1
  • Ivan Literák
    • 1
  1. 1.Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and EcologyUniversity of Veterinary and Pharmaceutical SciencesBrnoCzech Republic
  2. 2.Department of Virology and SerologyState Veterinary Institute PraguePrague 6Czech Republic

Personalised recommendations