Parasitology Research

, Volume 107, Issue 4, pp 783–785 | Cite as

Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic

  • Eva BártováEmail author
  • Kamil Sedlák
  • Michaela Syrová
  • Ivan Literák
Original Article


During January 2007, blood samples were collected from 552 healthy horses from nine different regions of the Czech Republic. Sera were tested for serum antibodies to Neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay and confirmed by an indirect fluorescent antibody test. The same samples were tested for serum antibodies against Toxoplasma gondii by a latex agglutination test. In total, 131 of 552 (24%) horses reacted positively for Neospora antibodies in competitive-inhibition enzyme-linked immunosorbent assay; seven of them had ≥50% of inhibition. Samples were confirmed in indirect fluorescence test, and only two samples were positive with final titres 50 and 100, while others were negative. Antibodies against T. gondii were found in 125 (23%) horses. This is the first serologic survey for Neospora spp. antibodies performed on horses in the Czech Republic.


Czech Republic Toxoplasmosis Definitive Host Toxoplasma Gondii Indirect Fluorescent Antibody Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported by the Ministry of Education, Youth and Sports of the Czech Republic (Grant No. MSM6215712402).


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Eva Bártová
    • 1
    Email author
  • Kamil Sedlák
    • 2
  • Michaela Syrová
    • 1
  • Ivan Literák
    • 1
  1. 1.Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and EcologyUniversity of Veterinary and Pharmaceutical SciencesBrnoCzech Republic
  2. 2.Department of Virology and SerologyState Veterinary Institute PraguePrague 6Czech Republic

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