Parasitology Research

, Volume 107, Issue 2, pp 459–463 | Cite as

Effects of in vitro exposure of Echinococcus multilocularis metacestodes to cytostatic drugs on in vivo growth and proliferation of the parasite

  • Cora Hübner
  • Stefan Wiehr
  • Lars Kocherscheidt
  • Hans Wehrl
  • Bernd J. Pichler
  • Andreas Schmid
  • Peter Kern
  • Peter T. Soboslay
Short Communication

Abstract

The cytostatic drugs Vincristine (VCR), Navelbine (NAV), and Methotrexate (MTX) were evaluated for their growth inhibitory potential against metacestodes of Echinococcus multilocularis (Em) by in vitro and in vivo assays. In vitro cultures of E. multilocularis were exposed to IC 90, IC 80, and IC 5 concentrations of VCR, NAV, or MTX for 1 week, then parasite tissue cultures were kept for 1 week without drug exposure in vitro, and thereafter, metacestode tissues were injected intra-peritoneally into Meriones unguiculatus. Metacestode growth was monitored for several months post-infection (p.i.) by body weight control, magnetic resonance imaging (MRI), and autopsy at 5 months p.i. Weight monitoring of infected M. unguiculatus did not provide conclusive evidence for Em-metacestode growth, while MRI could detect growing Em-metacestode in the MTX-treated group at 8 weeks (p.i.), whereas metacestodes exposed to VCR and NAV were at 17 weeks (p.i.) detectable. MRI disclosed progressive and massive growth of Em-metacestode in the VCR- and MTX-exposed groups, while the NAV-pretreated Em-metacestodes’ volume did not exceed 4 cm3. At autopsy, Em-metacestodes of less than 4 cm3 were found in infected M. unguiculatus, which was not detected by MRI. In summary, the cytostatic drugs Methotrexate, Navelbine, and Vincristine—as applied in the present work—did not show parasitocidal or clear parasitostatic effects on metacestodes of E. multilocularis. While parasite growth in vivo was inhibited in NAV- and VCR-pretreated Em-metacestodes, MTX pretreatment seemed to enhance parasite proliferation. Magnetic resonance imaging appears suitable to monitor in vivo the effects of drugs on growth progression and regression only of larger Em-metacestode tissues.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Cora Hübner
    • 1
  • Stefan Wiehr
    • 2
  • Lars Kocherscheidt
    • 1
  • Hans Wehrl
    • 2
  • Bernd J. Pichler
    • 2
  • Andreas Schmid
    • 2
  • Peter Kern
    • 3
  • Peter T. Soboslay
    • 1
  1. 1.Institute for Tropical MedicineUniversity Clinics of TübingenTübingenGermany
  2. 2.Laboratory for Preclinical Imaging and Imaging Technology of the Werner Siemens-FoundationEberhard-Karls-University TübingenTübingenGermany
  3. 3.Section of Clinical Immunology and InfectiologyUniversity Clinics UlmUlmGermany

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