Bioactivity of propolis from different geographical origins on Varroa destructor (Acari: Varroidae)
- 410 Downloads
Varroa destructor is an ectoparasitic mite that affects colonies of honey bee Apis mellifera worldwide. In the last years, substances of botanical origin have emerged as natural alternative acaricides to diminish the population levels of the mite. In the present work, the bioactivity of propolis from different geographical locations of Pampean region from Argentina on V. destructor was evaluated. Fourteen propolis samples were organoleptic and physicochemically characterized and, by means topical applications, their activity was tested on mites. All propolis had a homogeneous composition and the bioactivity levels against mites were comparable among the different propolis samples. The percentage of mites killed by the treatments ranged between 60.5% and 90% after 30 s of exposure. Thus, V. destructor was highly susceptible to propolis. Moreover, the mites remained anesthetized during the first hours after topical treatment. The results suggest that propolis from Argentinean pampas could be incorporated in honey bee colonies as acaricidal treatment by spraying.
KeywordsPropolis Botanical Origin Varroa Destructor Propolis Extract Pampean Region
We would to thank to all beekeepers that contributed to the propolis samples collections and the media involved in the called diffusion, Javier Folgar, Federico Petrera and their team. To Tec. Carlos Torne for the help in the physicochemical analyses of propolis samples in INTA Famailla, Tucumán. This study was support by PICT REDES Project Nº 00890 to ME (ANPCYT) and Exa Project Nº 376/07 to JM (UNMDP), Argentina.
- Bankova V, Marcucci MC, Simova S, Nikolova N, Kujumgiev A, Popov S (1996) Antibacterial diterpenic acids from Brazilian propolis. Z Naturforsch C 51(5–6):277–280Google Scholar
- Bedascarrasbure E, Maldonado L, Fierro Morales W, Álvarez A (2006) Propóleos. Caracterización y normalización de propóleos argentinos. Revisión y actualización de composición y propiedades. Ediciones Magna Publicaciones, TucumánGoogle Scholar
- Bogdanov S, Kolchenmann V, Imdorf A (1998) Acaricide residues in some bee products. J Apic Res 37:57–67Google Scholar
- Bonvehi JS, Coll FV (1994) Phenolic composition of propolis from China and from South America. Z Naturforsch C 49:712–718Google Scholar
- Dellacasa AD, Bailac PN, Ponzi MI, Ruffinengo SR, Eguaras MJ (2003) In vitro activity of essential oils against Ascosphaera apis. J Essent Oil Res 15(3):282–285Google Scholar
- Gende LB, Palazzetti M, Pannone O, Fritz R, Eguaras MJ, Saccares S, Dell’Aira E, Formato G (2007) Comparative field trials of Paenibacillus larvae control with antibiotics, essential oil of cinnamon and propolis. Proceedings of 40th Apimondia CongressGoogle Scholar
- Gende LB, Maggi M, Damiani N, Fritz R, Eguaras MJ, Floris I (2009) Advances in the apiary control of the honeybee American foulbrood with Cinnamon (Cinnamomun zeylanicum) essential oil. B Insectol 62(1):93–97Google Scholar
- IRAM-INTA Norms 15935-1 (2008) NOA (Argentinean northwest) products. Propolis. Part 1: Propolis in natura. pp 25Google Scholar
- Pohorecka K (2004) Laboratory studies on the effect of standardized Artemisia absinthium L. extract on Nosema apis infection in the worker Apis mellifera. J Apicult Sci 48(2):131–136Google Scholar