Parasitology Research

, Volume 106, Issue 6, pp 1485–1489 | Cite as

Effect of fingerroot on reducing inflammatory cells in hamster infected with Opisthorchis viverrini and N-nitrosodimethylamine administration

  • Sirintip Boonjaraspinyo
  • Thidarut Boonmars
  • Chantana Aromdee
  • Butsara Kaewsamut
Short Communication


The active compound in fingerroot is effective in the treatment of many inflammatory diseases. The aim of our present study was to evaluate the efficacy of fingerroot on reducing histopathological changes in hamsters that were infected with the liver fluke Opisthorchis viverrini or were administered N-nitrosodimethylamine (NDMA), and then treated with fingerroot. Light microscopic observation and liver function tests for alanine transaminase (ALT), alkaline phosphatase (ALP), and direct bilirubin were investigated. The results of histopathological changes show that fingerroot has anti-inflammatory properties—in the case of N-nitrosodimethylamine administration on day 30—by reducing the aggregation of inflammatory cells surrounding the hepatic bile ducts, which correlates with a decreased serum ALT level. The decrease of direct bilirubin levels in hamsters treated with fingerroot suggests that fingerroot may enhance biliary contraction. The present study found that fingerroot clearly reduces the inflammatory cells in hamsters that were administered NDMA, but not in the case of O. viverrini infection. This finding suggests that fingerroot has anti-inflammatory property, but not in the case of hamster opisthorchiasis.



This work was granted by the Thailand research fund through the Royal Golden Jubilee-Ph.D. program (Grant No. PHD/0139/2551) to Miss Sirintip Boonjaraspinyo and Assistant Professor Thidarut Boonmars and partial support from National Center for Genetic Engineering and Biotechnology (BIOTEC) and National Science and Technology Development Agency (NSTDA). We thank the Department of Parasitology, Liver Fluke and Cholangiocarcinoma Research Center, the Animal Experimental Unit, Faculty of Medicine, Khon Kaen University for their support.


  1. Boonjaraspinyo S, Boonmars T, Aromdee C, Srisawangwong T, Kaewsamut B, Pinlaor S, Yongvanit P, Puapairoj A (2009) Turmeric reduces inflammatory cells in hamster opisthorchiasis. Parasitol Res 105:1459–1463CrossRefPubMedGoogle Scholar
  2. Boonmars T, Boonjaraspinyo S, Kaewsamut B (2009) Animal models for Opisthorchis viverrini infection. Parasitol Res 104:701–703CrossRefPubMedGoogle Scholar
  3. Boonmars T, Srirach P, Kaewsamut B, Srisawangwong T, Pinlaor S, Pinlaor P, Yongvanit P, Sithithaworn P (2008) Apoptosis-related gene expression in hamster opisthorchiasis post praziquantel treatment. Parasitol Res 102:447–455CrossRefPubMedGoogle Scholar
  4. Boonmars T, Srisawangwong T, Srirach P, Kaewsamut B, Pinlaor S, Sithithaworn P (2007) Apoptosis-related gene expressions in hamsters re-infected with Opisthorchis viverrini and re-treated with praziquantel. Parasitol Res 102:57–62CrossRefPubMedGoogle Scholar
  5. Chandra K, Graham PJ, Ian RR, Graeme HM (2007) Anticancer properties of panduratin A isolated from Boesenbergia pandurata (Zingiberaceae). J Nat Med 61:131–137CrossRefGoogle Scholar
  6. Cuendet M, Oteham CP, Moon RC, Pezzuto JM (2006) Quinone reductase induction as a biomarker for cancer chemoprevention. J Nat Prod 69:460–463CrossRefPubMedGoogle Scholar
  7. Fahey JW, Stephenson KK (2002) Pinostrobin from honey and Thai ginger (Boesenbergia pandurata): a potent flavonoid inducer of mammalian phase 2 chemoprotective and antioxidant enzymes. J Agric Food Chem 50:7472–7476CrossRefPubMedGoogle Scholar
  8. George J, Rao KR, Stern R, Chandrakasan G (2001) Dimethylnitrosamine-induced liver injury in rats: the early deposition of collagen. Toxicology 156:129–138CrossRefPubMedGoogle Scholar
  9. IARC (1994) Infection with liver flukes (Opisthorchis viverrini, Opisthorchis felineus and Clonorchis sinensis). IARC Monogr Eval Carcinog Risks Hum 61:121–175Google Scholar
  10. Mahidol C, Tuntiwachwuttikul P, Reutrakul V, Taylor WC (1984) Constituents of Boesenbergia pandurata (syn. Kaempferia pandurata) III Isolation and synthesis of (±)-boesenbergin B. Aust J Chem 37:1739–1745CrossRefGoogle Scholar
  11. Murakami A, Jiwajinda S, Koshimizu K, Ohigashi H (1995) Screening for in vitro anti-tumor promoting activities of edible plants from Thailand. Cancer Lett 95:139–146CrossRefPubMedGoogle Scholar
  12. Peto R, Gray R, Brantom P, Grasso P (1991) Dose and time relationships for tumor induction in the liver and esophagus of 4080 inbred rats by chronic ingestion of N-nitrosodiethylamine or N-nitrosodimethylamine. Cancer Res 51:6452–6469PubMedGoogle Scholar
  13. Pinlaor S, Hiraku Y, Ma N, Yongvanit P, Semba R, Oikawa S, Murata M, Sripa B, Sithithaworn P, Kawanishi S (2004) Mechanism of NO-mediated oxidative and nitrative DNA damage in hamsters infected with Opisthorchis viverrini: a model of inflammation-mediated carcinogenesis. Nitric Oxide 11:175–183CrossRefPubMedGoogle Scholar
  14. Shindo K, Kato M, Kinoshita A, Kobayashi A, Koike Y (2006) Analysis of antioxidant activities contained in the Boesenbergia pandurata Schult. Rhizome. Biosci Biotechnol Biochem 70:2281–2284CrossRefPubMedGoogle Scholar
  15. Sripa B (2003) Pathobiology of opisthorchiasis: an update. Acta Tropica 88:209–220CrossRefPubMedGoogle Scholar
  16. Tuchinda P, Reutrakul V, Claeson P, Pongprayoon U, Sematong T, Santisuk T, Taylor WC (2002) Anti-inflammatory cyclohexenyl chalcone derivatives in Boesenbergia pandurata. Phytochemistry 59:169–173CrossRefPubMedGoogle Scholar
  17. Yanti A, Gwon SH, Hwang JK (2009) Kaempferia pandurata Roxb. inhibits Porphyromonas gingivalis supernatant-induced matrix metalloproteinase-9 expression via signal transduction in human oral epidermoid cells. J Ethnopharmacol 123:315–324CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Sirintip Boonjaraspinyo
    • 1
    • 2
  • Thidarut Boonmars
    • 1
    • 2
  • Chantana Aromdee
    • 3
  • Butsara Kaewsamut
    • 4
  1. 1.Department of Parasitology, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand
  2. 2.Liver Fluke and Cholangiocarcinoma Research Center, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand
  3. 3.Faculty of PharmacologyKhon Kaen UniversityKhon KaenThailand
  4. 4.Northeast Laboratory Animal CenterKhon Kaen UniversityKhon KaenThailand

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