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Parasitology Research

, Volume 106, Issue 5, pp 1225–1231 | Cite as

Immunoblot analysis of membrane antigens of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium against Schistosoma-infected patient sera

  • Italo M. Cesari
  • Diana E. Ballen
  • L. Mendoza
  • Alain Ferrer
  • Jean-Pierre Pointier
  • Maryvonne Kombila
  • Dominique Richard-Lenoble
  • Andre ThéronEmail author
Original Paper

Abstract

Antigens present in aqueous n-butanolic extracts (BE) of Schistosoma mansoni (Venezuelan JL strain), Schistosoma intercalatum (Cameroon EDEA strain), and Schistosoma haematobium (Yemen strain) adult worm membranes were compared in immunoblot against sera of patients infected with S. mansoni, S. intercalatum, S. haematobium, Schistosoma japonicum, or Schistosoma mekongi looking for similarities (common antigens) and differences (species-specific antigens). About 17 S. mansoni BE polypeptides (M r ∼8 to >80 kDa) were commonly recognized by S. mansoni-infected patient sera from Venezuela, Senegal, and Ethiopia. S. intercalatum-, S. haematobium-, or S. japonicum-infected sera were almost unreactive with S. mansoni BE. Nonetheless, S. mekongi-infected sera weakly cross-reacted with a ∼10–15-kDa subset of S. mansoni BE. About 72.7% of S. intercalatum-infected patient sera reacted with a ∼19–21-kDa complex in S. intercalatum BE and cross-reacted with a similar complex in S. haematobium BE. Conversely, all S. haematobium-infected patient sera reacted with a ∼19–21-kDa complex in S. haematobium BE and cross-reacted with the ∼19–21-kDa complex in S. intercalatum BE; S. mansoni- and S. japonicum-infected patient sera did not react with S. intercalatum or S. haematobium BE. Results showed the presence of a common membrane antigen between African schistosome species and species-specific antigens in S. mansoni BE that could be useful to discriminate between species and/or to detect Schistosoma infections.

Keywords

Schistosomiasis Adult Worm Schistosoma Patient Seron Schistosoma Haematobium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank the CNRS (France)-FONACIT (Venezuela) convention for financing the travels between France and Venezuela and ECOS-Nord V06A01. We thank Cesar Matos and Anne Rognon for their contribution to maintenance of life cycles of the schistosome species.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Italo M. Cesari
    • 1
  • Diana E. Ballen
    • 1
  • L. Mendoza
    • 1
  • Alain Ferrer
    • 2
  • Jean-Pierre Pointier
    • 4
  • Maryvonne Kombila
    • 3
  • Dominique Richard-Lenoble
    • 3
  • Andre Théron
    • 4
    Email author
  1. 1.Centro de Microbiología y Biología CelularInstituto Venezolano de Investigaciones Científicas (IVIC)CaracasVenezuela
  2. 2.Laboratoire de Parasitologie, Mycologie et Médecine TropicaleU.F.R. de MédecineTours cedexFrance
  3. 3.Département de Parasitologie–Mycologie–Médecine Tropicale Faculté de Médecine et des Sciences de la SantéUniversité des Sciences de la SantéLibrevilleGabon
  4. 4.UMR 5244 CNRS-EPHE-UPVD Biologie et Ecologie Tropicale et MéditerranéenneUniversité de Perpignan Via DomitiaPerpignan CedexFrance

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