Parasitology Research

, Volume 106, Issue 4, pp 985–989 | Cite as

Trypanocidal activity and acute toxicity assessment of triterpene acids

  • Daniele da Silva Ferreira
  • Viviane Rodrigues Esperandim
  • Miriam Paula Alonso Toldo
  • Juliana Saraiva
  • Wilson Roberto Cunha
  • Sérgio de Albuquerque
Short Communication


The present study evaluates the in vitro and in vivo trypanocidal activity of ursolic acid and oleanolic acid against the Bolivia strain of Trypanosoma cruzi. Their acute toxicity is also assessed on the basis of median lethal dose (DL50) determination and quantification of biochemical parameters. Ursolic acid is the most active compound in vitro, furnishing IC50 of 25.5 μM and displaying 77% of trypomastigote lysis at a concentration of 128 µM. In agreement with in vitro assays, the results obtained for the in vivo assay reveals that ursolic acid (at a dose of 20 mg/Kg/day) provides the most significant reduction in the number of parasites at the parasitemic peak. Results concerning the LD50 assay and the biochemical parameters evaluated in the present study demonstrate that these substances can be safely used on an experimental basis.


Ursolic Acid Oleanolic Acid Trypanosoma Cruzi Median Lethal Dose Triterpene Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We would like to thank CAPES, CNPq, and FAPESP for financial support.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Daniele da Silva Ferreira
    • 1
  • Viviane Rodrigues Esperandim
    • 1
  • Miriam Paula Alonso Toldo
    • 1
  • Juliana Saraiva
    • 1
  • Wilson Roberto Cunha
    • 2
  • Sérgio de Albuquerque
    • 1
  1. 1.Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São PauloRibeirão PretoBrazil
  2. 2.Núcleo de Pesquisa em Ciências Exatas e Tecnológicas da Universidade de FrancaFrancaBrazil

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