Parasitology Research

, Volume 105, Issue 6, pp 1653–1659 | Cite as

Evaluation of larvicidal nature of fleshy fruit wall of Momordica charantia Linn. (family: cucurbitaceae) in the management of mosquitoes

  • Prejwltta Maurya
  • Preeti Sharma
  • Lalit Mohan
  • Lata Batabyal
  • C. N. Srivastava
Original Paper


In view of the recently increased interest in developing plant-based insecticides as an alternative to chemical insecticides, this study was undertaken to assess the larvicidal potential of the various fruit wall extracts of Momordica charantia (cucurbitaceae) against two species of mosquito vectors, Anophels stephensi and Culex quinquefasciatus. Among the extracts tested, petroleum ether (LC50 = 27.60; 17.22 ppm and 41.36; 15.62 ppm) extract was found more effective than carbon tetrachloride (LC50 = 49.58; 16.15 ppm and 80.61; 27.64 ppm) and methanol (LC50 = 142.82; 95.98 ppm and 1,057.49; 579.93 ppm) extracts towards anopheline and culicine larvae after 24 and 48 h of exposure respectively. Thus, all fruit wall extracts of M. charantia are toxic to both the larval species. M. charantia may, therefore, act as an effective biolarvicide against mosquitoes in the future.



Authors are thankful to University Grant Commission, New Delhi for the financial assistance.


  1. Al-Sharook Z, Balan K, Jiang Y, Rembold H (1991) Insect growth inhibitors from two tropical meliaceae: effect of crude seed extracts on mosquito larvae. J Appl Entomol 111:425–430CrossRefGoogle Scholar
  2. Batabyal L, Sharma P, Mohan L, Maurya P, Srivastava CN (2007) Larvicidal efficiency of certain seed extracts against A. Stephensi, with reference to Azadirachta indica. J Asia Pacific Entomol 10:1–5CrossRefGoogle Scholar
  3. Bernhard L, Bernhard P, Magnussen P (2003) Management of patients with lymphoedema caused by filariasis in northeastern Tanzania: alternative approaches. Physiotherapy 89:743–749CrossRefGoogle Scholar
  4. Choochote W, Kanjanapothi D, Panthong A, Taesotikul T, Jitpakdi A, Chaithong U, Pitasawat B (1999) Larvicidal, adulticidal, and repellent effects of Kaempferia galanga Southeast Asian. J Trop Med Public Health 30:470–476Google Scholar
  5. Chopra RN (1933) Indigenous drugs of India. The Art Press 310, CalcuttaGoogle Scholar
  6. Chopra RN, Roy DN, Ghose SM (1941) The insecticidal and larvicidal action of the essential oils of the Ocimum basilicum (Linn.) and Ocimum sanctum (Linn.). J Malaria Inst India 4:109–112Google Scholar
  7. Chopra RN, Nayar SL, Chopra IC (1956) Glossary of Indian medicinal plants. CSIR 229, New DelhiGoogle Scholar
  8. Ciccia G, Coussio J, Mongelli E (2000) Insecticidal activity against Aedes aegypti larvae of some medicinal South American plants. J Ethnopharmacol 72:185–189CrossRefPubMedGoogle Scholar
  9. David JP, Rey D, Pautou MP, Meyran JC (2000) Differential toxicity of leaf litter to dipteran larvae of mosquito developmental sites. J Invertebr Pathol 75:9–18CrossRefPubMedGoogle Scholar
  10. Deshmukh PB, Chavan SR, Renapurkar DM (1982) Study of insecticidal activity of 20 indigenous plants. Pesticide 16:7–10Google Scholar
  11. Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, CambridgeGoogle Scholar
  12. Fradin MS, Day JF (2002) Comparative efficacy of insect repellents against mosquitoes bites. N Engl J Med 347:13–18CrossRefPubMedGoogle Scholar
  13. George S, Vincent S (2005) Comparative efficacy of Annona squamosa Linn. and Pongamia glabra Vent. to Azadirachta indica A. Juss against mosquitoes. J Vect Borne Dis 42:59–163Google Scholar
  14. Jang YS, Baek BR, Yang YC, Kim MK, Lee HS (2002) Larvicidal activity of leguminous seeds and grains against Aedes aegypti and C. pipiens pallens. J Am Mosq Control Assoc 18:210–213PubMedGoogle Scholar
  15. Kumar A, Tewari GD, Pandey ND (1979) Studies on antifeeding and insecticidal properties of bitter gourd (Momordica charantia Linn) against mustard saw fly, Athalia proxima Klug. Pesticides 13:9Google Scholar
  16. Macedo J, Consoli RAGB, Grandi TSM, Dos Anjos AMG, de Olivira AB, Mendes Nm, Queiroz RO, Zani CL (1997) Screening of Asteraceae (Compostae) plant extracts for larvicidal activity aginst Aedes fluviatilis (Diptera: Culicidae). Mem Inst Oswaldo Cruz 92:565–570CrossRefPubMedGoogle Scholar
  17. Mansour SA, Messeha SS, EL-Gengaihi SE (2000) Botanical biocides 4 Mosquitocidal activity of certain Thymus capitatus constituents. J Nat Toxins 9:49–62PubMedGoogle Scholar
  18. Markouk M, Bekkouche K, Larhsini M, Bousaid M, Lazrek HB, Jana M (2000) Evaluation of some Moroccan medicinal plant extracts for larvicidal activity. J Ethnopharmacol 73:293–297CrossRefPubMedGoogle Scholar
  19. Mishra D, Shukla AK, Debey AK, Dixit AK, Singh K (2006) Insecticidal activity of vegetable oil against mustard aphid, Lipaphis erysimi Kalt. under field condition. J Oleo Sci 55:227–231Google Scholar
  20. Mohan L, Sharma P, Srivastava CN (2005) Evaluation of Solanum xanthocarpum extracts as mosquito larvicides. J Environ Biol 26:399–401PubMedGoogle Scholar
  21. Mwangi RW, Rembold H (1988) Growth-inhibiting and larvicidal effects of Melia volkensii extracts on Aedes aegypti larvae. Entomol Exp Appl 46:103–108CrossRefGoogle Scholar
  22. Nivsarkar M, Cherian B, Padh H (2001) Alpha-terthienyl: a plant-derived new generation insecticide. Current Science 81:667–672Google Scholar
  23. Njan Nloga AM (1994) Bioecology and role of malaria vector of A. moucheti at Ebogo-Cameroon. Ph.D. Thesis, University of Montpellier II, Parasitology 221Google Scholar
  24. Perich MJ, Wells C, Bertsch W, Tredway KE (1994) Isolation of the insecticidal components of Tagetes minuta (Compositae) against mosquito larvae and adults. J Am Mosq Control Assoc 11:307–310Google Scholar
  25. Prabakar K, Jebanesan A (2004) Larvicidal efficacy of some Cucurbitaceous plant leaf extracts against C. quinquefasciatus (Say). Bioresour Technol 95:113–114CrossRefPubMedGoogle Scholar
  26. Pushpalatha E, Muthukrishnan J (1999) Efficacy of two tropical plant extracts for the control of mosquitoes. J Appl Entomol 123:369–373CrossRefGoogle Scholar
  27. Rahuman AA, Gopalakrishnan G, Venkatesan P, Kannappan G (2008a) Isolation and identification of mosquito larvicidal compound from abutilon indicum (linn.) Sweet. Parasitol Res 102:981–988CrossRefPubMedGoogle Scholar
  28. Rahuman AA, Gopalakrishnan G, Venkatesan P, Kannappan G (2008b) Larvicidal activity of some euphorbiaceae plant extracts against Aedes aegypti and C. quinquefasciatus (Diptera: Culicidae). Parasitol Res 102:867–873CrossRefPubMedGoogle Scholar
  29. Ranaweera SS (1996) Mosquito–larvicidal activity of some Sri Lankan plants. J Natl Sci Counc Sri Lanka 24:63–69Google Scholar
  30. Rey D, Pautou M-P, Meyran J-C (1999) Histopathological effects of tannic acid on the midgut epithelium of some aquatic Diptera larvae. J Invertebr Pathol 73:173–181CrossRefPubMedGoogle Scholar
  31. Rey D, David JP, Besnard G, Jullien JL, Lagneau C, Meyran JC (2001) Comparative sensitivity of larval mosquito to vegetable polyphenols versus conventional insecticides. Entomol Exp Appl 98:361–367CrossRefGoogle Scholar
  32. Shaalan E, Canyon DV, Faried MW, Abdel-Wahab H, Mansour A (2003) Efficacy of a highly active phytochemical (Callitris glaucophylla) against mosquito vectors of dengue and Japanese encephalitis. The Annual Queensland Health and Medical Scientific Meeting, “Making It Better: Encouraging health research and innovation.” 25–26 November, BrisbaneGoogle Scholar
  33. Shaleen EA, Canyon DV, Younes MW, Abdel-Wahab H, Mansour AH (2006) Efficacy of eight larvicidal botanical extracts from Khaya Senegalensis and Daucus carota against C. annulirostris. J Am Mosq Control Assoc 22:433–436CrossRefGoogle Scholar
  34. Sharma RN, Deshpande SG, Tungikar VB, Joseph M (1994) Toxicity of natural essential oils to mosquitoes, Aedes aegypti and C. fatigans. Geobios 21:162–165Google Scholar
  35. Sharma P, Mohan L, Srivastava CN (2004) Larval susceptibility of Ajuga remota against anopheline and culicine mosquitoes. Southeast Asian J Trop Med Public Health 35:608–610PubMedGoogle Scholar
  36. Sharma P, Mohan L, Srivastava CN (2006) Impact analysis of neem kernel extracts on the development profile of A. stephensi. J Asia Pacific Entomol 9:11–17CrossRefGoogle Scholar
  37. Singh RK, Dhiman RC, Mittal PK (2006) Mosquito larvicidal properties of Momordica charantia Linn (Family: Cucurbitaceae). J Vect Borne Dis 43:88–91Google Scholar
  38. Thangam TS, Kathiresan K (1991) Mosquito larvicidal activity of marine plant extracts with insecticides. Bot Mar 34:537–539CrossRefGoogle Scholar
  39. Thomas CJ, Callaghan A (1999) The use of garlic (Allium sativa) and lemon peel (Citrus limon) extracts as C. pipiens larvicides: persistence and interaction with an organophosphate resistance mechanism. Chemosphere 39:2489–2496CrossRefGoogle Scholar
  40. Traboulsi AF, Taoubi K, el-Haj S, Bessiere JM, Rammal S (2002) Insecticidal properties of essential plant oils against the mosquito C. pipiens molestus (Diptera: Culicidae). Pest Manag Sci 58:491–495CrossRefPubMedGoogle Scholar
  41. Traboulsi AF, El-Hay S, Tueni M, Taoubi K, Nader NA, Mrad A (2005) Repellency and toxicity of aromatic plant extracts against the mosquito Cx. Pipiens Molestus (Diptera-Culicidae). Pest Manag Sci 61:597–604CrossRefPubMedGoogle Scholar
  42. Weisman Z, Chapagam PB (2006) Larvicidal activity of saponin containing extracts and fractions of fruit mesocarp of Balanites aegyptiaca. Fitoterapia 77:420–424CrossRefGoogle Scholar
  43. WHO (1981) Instructions for determinig the susceptibility or resistance of mosquito larvae to insecticides. Mimeographed document WHO/VBC/81.807Google Scholar
  44. WHO (2003) World Health Organization Fact Sheet No. 94. WHO Information,∼fs/en/fact094.html
  45. Yang Y, Lee S, Lee H, Kim M, Lee S, Lee H (2002) A piperidine amide extracted from Piper longum L fruit shows activity against Aedes aegypti mosquito larvae. J Agric Food Chem 50:3765–3767CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Prejwltta Maurya
    • 1
  • Preeti Sharma
    • 1
  • Lalit Mohan
    • 1
  • Lata Batabyal
    • 1
  • C. N. Srivastava
    • 1
  1. 1.Applied Entomology and Vector Control Laboratory, Department of Zoology, Faculty of ScienceDayalbagh Educational Institute (Deemed University)AgraIndia

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