Parasitology Research

, 105:1661 | Cite as

Cloning, characterization, and expression of a novel secretory lipase-like gene from Clonorchis sinensis

  • Fengyu Hu
  • Wanshan Chen
  • Linhua Li
  • Yecheng Lu
  • Weinan Song
  • Yanling Kuang
  • Fuchun Zhang
Original Paper
First Online:
Received:
Accepted:

Abstract

A secretory lipase-like gene was isolated from total cDNA of adult Clonorchis sinensis. The gene has an open reading frame of 1,218 bp long and encodes for a protein of 406 amino acids including a putative signal peptide of 20 amino acids. The deduced amino acid sequence including signal peptide has 42–45% identity with lipase of other species and two typical enzymic active sites that contain consensus sequence (Gly-X-Ser-X-Gly) of lipase. The cDNA encoding this protein was subcloned into pET-28a (+) expression vector and expressed in Escherichia coli. The expressed fusion protein has a molecular mass of about 45 kDa. Prediction of signal peptide and Western blot analysis indicated that the secretory lipase-like protein is an excretory–secretory product of C. sinensis. Immunostaining revealed that the secretory lipase-like protein was localized in the tegument of the adult worm and metacercaria. These results provide basis for further studies on the nutrition taking and invasion of C. sinensis mediated by the secretory lipase-like protein.

Keywords

Lipase Adult Worm Ventral Sucker Purify Polymerase Chain Reaction Product Clonorchiasis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

This work was financially supported by the key science and technique program of Guangdong province, China (no. 2004A30801004), and Guangdong province technology program (no. 2004B36001027).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Fengyu Hu
    • 1
  • Wanshan Chen
    • 1
  • Linhua Li
    • 1
  • Yecheng Lu
    • 1
  • Weinan Song
    • 1
  • Yanling Kuang
    • 1
  • Fuchun Zhang
    • 1
  1. 1.Institute of Infectious DiseasesGuangzhou No. 8 People’s HospitalGuangzhouPeople’s Republic of China

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