Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia
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Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation–immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8% of samples contained low numbers of Giardia cysts (range, 0.17–1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water.
KeywordsRecreational Water Cryptosporidium Oocyst Immunomagnetisable Separation Giardia Cyst Faecal Coliform Count
The authors are very grateful to Mr. Wan Hafiz WI for technical assistance during sample collection.
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