Parasitology Research

, Volume 106, Issue 1, pp 289–291 | Cite as

Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia

  • Yvonne A. L. LimEmail author
  • Sharmila D. Ramasame
  • Mohammed A. K. Mahdy
  • Wan Yusoff W. Sulaiman
  • Huw V. Smith
Short Communication


Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation–immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8% of samples contained low numbers of Giardia cysts (range, 0.17–1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water.


Recreational Water Cryptosporidium Oocyst Immunomagnetisable Separation Giardia Cyst Faecal Coliform Count 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are very grateful to Mr. Wan Hafiz WI for technical assistance during sample collection.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Yvonne A. L. Lim
    • 1
    Email author
  • Sharmila D. Ramasame
    • 2
  • Mohammed A. K. Mahdy
    • 1
    • 3
  • Wan Yusoff W. Sulaiman
    • 1
  • Huw V. Smith
    • 4
  1. 1.Department of Parasitology, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia
  2. 2.Department of Microbiology, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia
  3. 3.Department of Parasitology, Faculty of MedicineSana’a UniversitySana’aYemen
  4. 4.Scottish Parasite Diagnostic LaboratoryStobhill HospitalGlasgowUK

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